Bismark report for: /gscratch/scrubbed/samwhite/data/C_gigas/BSseq/cgig_bsseq_all_R1.fastq.gz (version: v0.19.0) Option '--directional' specified (default mode): alignments to complementary strands (CTOT, CTOB) were ignored (i.e. not performed) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sam/data/C_gigas/genomes/ with the specified options: -q --score-min L,0,-0.6 -p 28 --reorder --ignore-quals Final Alignment report ====================== Sequences analysed in total: 5000000 Number of alignments with a unique best hit from the different alignments: 2747063 Mapping efficiency: 54.9% Sequences with no alignments under any condition: 1043852 Sequences did not map uniquely: 1209085 Sequences which were discarded because genomic sequence could not be extracted: 92 Number of sequences with unique best (first) alignment came from the bowtie output: CT/CT: 1373378 ((converted) top strand) CT/GA: 1373593 ((converted) bottom strand) GA/CT: 0 (complementary to (converted) top strand) GA/GA: 0 (complementary to (converted) bottom strand) Number of alignments to (merely theoretical) complementary strands being rejected in total: 0 Final Cytosine Methylation Report ================================= Total number of C's analysed: 23218150 Total methylated C's in CpG context: 924940 Total methylated C's in CHG context: 145279 Total methylated C's in CHH context: 276054 Total methylated C's in Unknown context: 4421 Total unmethylated C's in CpG context: 2629317 Total unmethylated C's in CHG context: 4788146 Total unmethylated C's in CHH context: 14454414 Total unmethylated C's in Unknown context: 38351 C methylated in CpG context: 26.0% C methylated in CHG context: 2.9% C methylated in CHH context: 1.9% C methylated in Unknown context (CN or CHN): 10.3% Bismark completed in 0d 0h 25m 23s