Bismark report for: /gscratch/scrubbed/samwhite/data/C_gigas/BSseq/cgig_bsseq_all_R1.fastq.gz (version: v0.19.0) Option '--directional' specified (default mode): alignments to complementary strands (CTOT, CTOB) were ignored (i.e. not performed) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sam/data/C_gigas/genomes/ with the specified options: -q --score-min L,0,-0.6 -p 28 --reorder --ignore-quals Final Alignment report ====================== Sequences analysed in total: 500000 Number of alignments with a unique best hit from the different alignments: 275445 Mapping efficiency: 55.1% Sequences with no alignments under any condition: 102993 Sequences did not map uniquely: 121562 Sequences which were discarded because genomic sequence could not be extracted: 7 Number of sequences with unique best (first) alignment came from the bowtie output: CT/CT: 137634 ((converted) top strand) CT/GA: 137804 ((converted) bottom strand) GA/CT: 0 (complementary to (converted) top strand) GA/GA: 0 (complementary to (converted) bottom strand) Number of alignments to (merely theoretical) complementary strands being rejected in total: 0 Final Cytosine Methylation Report ================================= Total number of C's analysed: 2323933 Total methylated C's in CpG context: 90514 Total methylated C's in CHG context: 14428 Total methylated C's in CHH context: 27427 Total methylated C's in Unknown context: 399 Total unmethylated C's in CpG context: 262264 Total unmethylated C's in CHG context: 476749 Total unmethylated C's in CHH context: 1452551 Total unmethylated C's in Unknown context: 3906 C methylated in CpG context: 25.7% C methylated in CHG context: 2.9% C methylated in CHH context: 1.9% C methylated in Unknown context (CN or CHN): 9.3% Bismark completed in 0d 0h 2m 50s