Bismark report for: /gscratch/scrubbed/samwhite/data/C_gigas/BSseq/cgig_bsseq_all_R1.fastq.gz (version: v0.19.0) Option '--directional' specified (default mode): alignments to complementary strands (CTOT, CTOB) were ignored (i.e. not performed) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sam/data/C_gigas/genomes/ with the specified options: -q --score-min L,0,-0.6 -p 28 --reorder --ignore-quals Final Alignment report ====================== Sequences analysed in total: 1000000 Number of alignments with a unique best hit from the different alignments: 550855 Mapping efficiency: 55.1% Sequences with no alignments under any condition: 206739 Sequences did not map uniquely: 242406 Sequences which were discarded because genomic sequence could not be extracted: 13 Number of sequences with unique best (first) alignment came from the bowtie output: CT/CT: 275768 ((converted) top strand) CT/GA: 275074 ((converted) bottom strand) GA/CT: 0 (complementary to (converted) top strand) GA/GA: 0 (complementary to (converted) bottom strand) Number of alignments to (merely theoretical) complementary strands being rejected in total: 0 Final Cytosine Methylation Report ================================= Total number of C's analysed: 4651517 Total methylated C's in CpG context: 183107 Total methylated C's in CHG context: 29107 Total methylated C's in CHH context: 55066 Total methylated C's in Unknown context: 837 Total unmethylated C's in CpG context: 525499 Total unmethylated C's in CHG context: 956115 Total unmethylated C's in CHH context: 2902623 Total unmethylated C's in Unknown context: 7744 C methylated in CpG context: 25.8% C methylated in CHG context: 3.0% C methylated in CHH context: 1.9% C methylated in Unknown context (CN or CHN): 9.8% Bismark completed in 0d 0h 5m 19s