Bismark report for: /gscratch/scrubbed/samwhite/data/C_gigas/BSseq/cgig_bsseq_pe_all_R1.fastq.gz and /gscratch/scrubbed/samwhite/data/C_gigas/BSseq/cgig_bsseq_pe_all_R2.fastq.gz (version: v0.19.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sam/data/C_gigas/genomes/ with the specified options: -q --score-min L,0,-0.6 -p 28 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--directional' specified (default mode): alignments to complementary strands (CTOT, CTOB) were ignored (i.e. not performed) Final Alignment report ====================== Sequence pairs analysed in total: 10000000 Number of paired-end alignments with a unique best hit: 5047522 Mapping efficiency: 50.5% Sequence pairs with no alignments under any condition: 4108088 Sequence pairs did not map uniquely: 844390 Sequence pairs which were discarded because genomic sequence could not be extracted: 456 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 2528514 ((converted) top strand) GA/CT/CT: 0 (complementary to (converted) top strand) GA/CT/GA: 0 (complementary to (converted) bottom strand) CT/GA/GA: 2518552 ((converted) bottom strand) Number of alignments to (merely theoretical) complementary strands being rejected in total: 0 Final Cytosine Methylation Report ================================= Total number of C's analysed: 105307378 Total methylated C's in CpG context: 1947531 Total methylated C's in CHG context: 44570 Total methylated C's in CHH context: 155977 Total methylated C's in Unknown context: 10465 Total unmethylated C's in CpG context: 10236643 Total unmethylated C's in CHG context: 17172924 Total unmethylated C's in CHH context: 75749733 Total unmethylated C's in Unknown context: 206032 C methylated in CpG context: 16.0% C methylated in CHG context: 0.3% C methylated in CHH context: 0.2% C methylated in unknown context (CN or CHN): 4.8% Bismark completed in 0d 1h 37m 9s