Bismark report for: /gscratch/scrubbed/samwhite/data/C_gigas/BSseq/cgig_bsseq_pe_all_R1.fastq.gz and /gscratch/scrubbed/samwhite/data/C_gigas/BSseq/cgig_bsseq_pe_all_R2.fastq.gz (version: v0.19.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sam/data/C_gigas/genomes/ with the specified options: -q --score-min L,0,-0.6 -p 28 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--directional' specified (default mode): alignments to complementary strands (CTOT, CTOB) were ignored (i.e. not performed) Final Alignment report ====================== Sequence pairs analysed in total: 100000 Number of paired-end alignments with a unique best hit: 20466 Mapping efficiency: 20.5% Sequence pairs with no alignments under any condition: 72323 Sequence pairs did not map uniquely: 7211 Sequence pairs which were discarded because genomic sequence could not be extracted: 2 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 10348 ((converted) top strand) GA/CT/CT: 0 (complementary to (converted) top strand) GA/CT/GA: 0 (complementary to (converted) bottom strand) CT/GA/GA: 10116 ((converted) bottom strand) Number of alignments to (merely theoretical) complementary strands being rejected in total: 0 Final Cytosine Methylation Report ================================= Total number of C's analysed: 216463 Total methylated C's in CpG context: 3093 Total methylated C's in CHG context: 41 Total methylated C's in CHH context: 156 Total methylated C's in Unknown context: 1 Total unmethylated C's in CpG context: 23065 Total unmethylated C's in CHG context: 35983 Total unmethylated C's in CHH context: 154125 Total unmethylated C's in Unknown context: 206 C methylated in CpG context: 11.8% C methylated in CHG context: 0.1% C methylated in CHH context: 0.1% C methylated in unknown context (CN or CHN): 0.5% Bismark completed in 0d 0h 0m 40s