SEQUENCE_ID=AF177226.1_cds_AAF20053.1_12 SEQUENCE_TEMPLATE=ATGTCAACAAATCATTTAGACATTGGAAGGTTTTATATAGTATTTGGATTTTGAGCTGTTCTTGCGGGAACTAGGTTTAGGTCTCTTATTCGTTGGAGACTTTATAACCCTGGAGCTAAGTTTTTAGACCCCGTGACTTATAATGCAGTTGTAACTAGGCATGCGTTGGTTATGATTTTTTTCTTTGTTATACCTGTAATAATTGGGGGGTTTGGTAACTGGCTTATCCCTTTGATGCTTCTAGTAGCAGACATGCAATTTCCTCGATTAAATGCATTTAGATTTTGAGTTTTGCCAGGGTCTCTTTATCTTATGCTTATGTCTAACATTGTAGAAAACGGAGTTGGGGCAGGGTGAACAATTTACCCTCCTTTATCAACTTACTCTTATCATGGAGTTTGTATAGACCTTGCAATTCTAAGCCTTCACCTTGCTGGTATTAGCTCTATTTTCAGGTCAATTAATTTCATAGTAACGATTAGAAATATGCGATCTGTTGGGGGCCATTTACTAGCACTATTCCCTTGATCTATTAAGGTTACTTCATTCTTGCTTTTGACTACTCTCCCAGTGTTAGCTGGAGGTCTTACTATACTTTTGACTGATCGTCATTTTAATACCTCTTTTTTTGACCCTGTCGGAGGGGGGGACCCTGTCTTATTTCAGCATTTGTTTTGATTTTTTGGTCACCCTGAGGTGTATGTCCTTATTCTTCCAGGTTTTGGAATAATTTCTCATGTCTTATGTTTTTGGTCAAGTAAAAAGACTGCATATGGAAATATGGGAATGTTTTATGCAATACTTAATATTGGGTTCTTAGGGTTTATTGTCTGGGGGCATCACATGTTTGTGGCTGGAATGGATATTGATACGCGTGCTTATTTTAGTGCTGCCACCGTTATTATTGCAGTGCCAACTGGTATTAAGGTGTTTGCATGAATTAGCACAATGCTAGGCTCTAAAGTTTCAACTCAAGCACCTATGTTGTGGTCTACTGGTTTTATTATTCTTTTTACAACAGGGGGTCTTACAGGACTTATTCTATCAAGAGCTTCAGTAGATGTTACGCTTCACGACACTTATTTTGTAACTGGTCATTTTCACTACGTCTTATCAATGGGTGCGGTGTTTACAATTTTAGCTGGGTTTACTCACTGATTTCCTCTTGTTGCTAAGGTTATAATGCATCGGCAAAAAATGAAAAGTCATTTTTTAGCAATGTTTTTAGGTGTTAATGCAGCATTTTTGCCACATCATTTTTTGGGTTTGGCTGGTATACCACGTCGAGTAGTTGATTATCCAGATCATTTTTGATTTTGAAATAAAGTATCCACATTTGGCTCTCATTTGAGTACTGGCTCATTGTTATTTTTTGTGTTTTTGTTATGAGAGTCATTTATTGCTCAACGGCCAGTTATTTCAGTGCGAAACACTTCTAGGTCCCCCGAATGGGCTGTTGTGTCTAGCCTCCCTAAGCATGCAGGGGATGAATTAGCAAAAATGGCTAAGCTTTGTTAG PRIMER_TASK=generic PRIMER_PICK_LEFT_PRIMER=3 PRIMER_PICK_RIGHT_PRIMER=3 PRIMER_OPT_SIZE=18 PRIMER_MIN_SIZE=15 PRIMER_MAX_SIZE=21 PRIMER_MAX_NS_ACCEPTED=1 PRIMER_PRODUCT_SIZE_RANGE=75-150 P3_FILE_FLAG=1 PRIMER_EXPLAIN_FLAG=1 PRIMER_THERMODYNAMIC_PARAMETERS_PATH=/home/sam/software/primer3-2.4.0/src/primer3_config/ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=1.578641 PRIMER_LEFT_0_PENALTY=1.463375 PRIMER_RIGHT_0_PENALTY=0.115266 PRIMER_LEFT_0_SEQUENCE=GGGGGTTTGGTAACTGGCT PRIMER_RIGHT_0_SEQUENCE=CCTGCCCCAACTCCGTTT PRIMER_LEFT_0=205,19 PRIMER_RIGHT_0=352,18 PRIMER_LEFT_0_TM=59.537 PRIMER_RIGHT_0_TM=59.885 PRIMER_LEFT_0_GC_PERCENT=57.895 PRIMER_RIGHT_0_GC_PERCENT=61.111 PRIMER_LEFT_0_SELF_ANY_TH=0.00 PRIMER_RIGHT_0_SELF_ANY_TH=0.00 PRIMER_LEFT_0_SELF_END_TH=0.00 PRIMER_RIGHT_0_SELF_END_TH=0.00 PRIMER_LEFT_0_HAIRPIN_TH=34.59 PRIMER_RIGHT_0_HAIRPIN_TH=0.00 PRIMER_LEFT_0_END_STABILITY=4.7500 PRIMER_RIGHT_0_END_STABILITY=3.6000 PRIMER_PAIR_0_COMPL_ANY_TH=0.00 PRIMER_PAIR_0_COMPL_END_TH=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=148 PRIMER_PAIR_1_PENALTY=2.222295 PRIMER_LEFT_1_PENALTY=2.107029 PRIMER_RIGHT_1_PENALTY=0.115266 PRIMER_LEFT_1_SEQUENCE=GGGGGTTTGGTAACTGGC PRIMER_RIGHT_1_SEQUENCE=CCTGCCCCAACTCCGTTT PRIMER_LEFT_1=205,18 PRIMER_RIGHT_1=352,18 PRIMER_LEFT_1_TM=57.893 PRIMER_RIGHT_1_TM=59.885 PRIMER_LEFT_1_GC_PERCENT=61.111 PRIMER_RIGHT_1_GC_PERCENT=61.111 PRIMER_LEFT_1_SELF_ANY_TH=0.00 PRIMER_RIGHT_1_SELF_ANY_TH=0.00 PRIMER_LEFT_1_SELF_END_TH=0.00 PRIMER_RIGHT_1_SELF_END_TH=0.00 PRIMER_LEFT_1_HAIRPIN_TH=34.59 PRIMER_RIGHT_1_HAIRPIN_TH=0.00 PRIMER_LEFT_1_END_STABILITY=4.8500 PRIMER_RIGHT_1_END_STABILITY=3.6000 PRIMER_PAIR_1_COMPL_ANY_TH=0.00 PRIMER_PAIR_1_COMPL_END_TH=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=148 PRIMER_PAIR_2_PENALTY=2.294210 PRIMER_LEFT_2_PENALTY=2.178944 PRIMER_RIGHT_2_PENALTY=0.115266 PRIMER_LEFT_2_SEQUENCE=GGGGGTTTGGTAACTGGCTT PRIMER_RIGHT_2_SEQUENCE=CCTGCCCCAACTCCGTTT PRIMER_LEFT_2=205,20 PRIMER_RIGHT_2=352,18 PRIMER_LEFT_2_TM=60.179 PRIMER_RIGHT_2_TM=59.885 PRIMER_LEFT_2_GC_PERCENT=55.000 PRIMER_RIGHT_2_GC_PERCENT=61.111 PRIMER_LEFT_2_SELF_ANY_TH=0.00 PRIMER_RIGHT_2_SELF_ANY_TH=0.00 PRIMER_LEFT_2_SELF_END_TH=0.00 PRIMER_RIGHT_2_SELF_END_TH=0.00 PRIMER_LEFT_2_HAIRPIN_TH=34.59 PRIMER_RIGHT_2_HAIRPIN_TH=0.00 PRIMER_LEFT_2_END_STABILITY=4.3500 PRIMER_RIGHT_2_END_STABILITY=3.6000 PRIMER_PAIR_2_COMPL_ANY_TH=0.00 PRIMER_PAIR_2_COMPL_END_TH=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=148 PRIMER_PAIR_3_PENALTY=2.632121 PRIMER_LEFT_3_PENALTY=2.507969 PRIMER_RIGHT_3_PENALTY=0.124151 PRIMER_LEFT_3_SEQUENCE=AAGCCTTCACCTTGCTGG PRIMER_RIGHT_3_SEQUENCE=GCCCCCAACAGATCGCAT PRIMER_LEFT_3=419,18 PRIMER_RIGHT_3=503,18 PRIMER_LEFT_3_TM=57.492 PRIMER_RIGHT_3_TM=60.124 PRIMER_LEFT_3_GC_PERCENT=55.556 PRIMER_RIGHT_3_GC_PERCENT=61.111 PRIMER_LEFT_3_SELF_ANY_TH=0.00 PRIMER_RIGHT_3_SELF_ANY_TH=0.00 PRIMER_LEFT_3_SELF_END_TH=0.00 PRIMER_RIGHT_3_SELF_END_TH=0.00 PRIMER_LEFT_3_HAIRPIN_TH=42.93 PRIMER_RIGHT_3_HAIRPIN_TH=0.00 PRIMER_LEFT_3_END_STABILITY=4.8500 PRIMER_RIGHT_3_END_STABILITY=4.7300 PRIMER_PAIR_3_COMPL_ANY_TH=0.00 PRIMER_PAIR_3_COMPL_END_TH=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=85 PRIMER_PAIR_4_PENALTY=2.705294 PRIMER_LEFT_4_PENALTY=0.197324 PRIMER_RIGHT_4_PENALTY=2.507969 PRIMER_LEFT_4_SEQUENCE=GTTGGGGCAGGGTGAACA PRIMER_RIGHT_4_SEQUENCE=CCAGCAAGGTGAAGGCTT PRIMER_LEFT_4=342,18 PRIMER_RIGHT_4=436,18 PRIMER_LEFT_4_TM=59.803 PRIMER_RIGHT_4_TM=57.492 PRIMER_LEFT_4_GC_PERCENT=61.111 PRIMER_RIGHT_4_GC_PERCENT=55.556 PRIMER_LEFT_4_SELF_ANY_TH=0.00 PRIMER_RIGHT_4_SELF_ANY_TH=0.00 PRIMER_LEFT_4_SELF_END_TH=0.00 PRIMER_RIGHT_4_SELF_END_TH=0.00 PRIMER_LEFT_4_HAIRPIN_TH=0.00 PRIMER_RIGHT_4_HAIRPIN_TH=35.27 PRIMER_LEFT_4_END_STABILITY=3.1800 PRIMER_RIGHT_4_END_STABILITY=4.3500 PRIMER_PAIR_4_COMPL_ANY_TH=0.00 PRIMER_PAIR_4_COMPL_END_TH=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=95 =