PRIMER PICKING RESULTS FOR AF177226.1_cds_AAF20053.1_12 No mispriming library specified Using 0-based sequence positions OLIGO start len tm gc% any_th 3'_th hairpin seq LEFT PRIMER 205 19 59.54 57.89 0.00 0.00 34.59 GGGGGTTTGGTAACTGGCT RIGHT PRIMER 352 18 59.88 61.11 0.00 0.00 0.00 CCTGCCCCAACTCCGTTT SEQUENCE SIZE: 1518 INCLUDED REGION SIZE: 1518 PRODUCT SIZE: 148, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00 0 ATGTCAACAAATCATTTAGACATTGGAAGGTTTTATATAGTATTTGGATTTTGAGCTGTT 60 CTTGCGGGAACTAGGTTTAGGTCTCTTATTCGTTGGAGACTTTATAACCCTGGAGCTAAG 120 TTTTTAGACCCCGTGACTTATAATGCAGTTGTAACTAGGCATGCGTTGGTTATGATTTTT 180 TTCTTTGTTATACCTGTAATAATTGGGGGGTTTGGTAACTGGCTTATCCCTTTGATGCTT >>>>>>>>>>>>>>>>>>> 240 CTAGTAGCAGACATGCAATTTCCTCGATTAAATGCATTTAGATTTTGAGTTTTGCCAGGG 300 TCTCTTTATCTTATGCTTATGTCTAACATTGTAGAAAACGGAGTTGGGGCAGGGTGAACA <<<<<<<<<<<<<<<<<< 360 ATTTACCCTCCTTTATCAACTTACTCTTATCATGGAGTTTGTATAGACCTTGCAATTCTA 420 AGCCTTCACCTTGCTGGTATTAGCTCTATTTTCAGGTCAATTAATTTCATAGTAACGATT 480 AGAAATATGCGATCTGTTGGGGGCCATTTACTAGCACTATTCCCTTGATCTATTAAGGTT 540 ACTTCATTCTTGCTTTTGACTACTCTCCCAGTGTTAGCTGGAGGTCTTACTATACTTTTG 600 ACTGATCGTCATTTTAATACCTCTTTTTTTGACCCTGTCGGAGGGGGGGACCCTGTCTTA 660 TTTCAGCATTTGTTTTGATTTTTTGGTCACCCTGAGGTGTATGTCCTTATTCTTCCAGGT 720 TTTGGAATAATTTCTCATGTCTTATGTTTTTGGTCAAGTAAAAAGACTGCATATGGAAAT 780 ATGGGAATGTTTTATGCAATACTTAATATTGGGTTCTTAGGGTTTATTGTCTGGGGGCAT 840 CACATGTTTGTGGCTGGAATGGATATTGATACGCGTGCTTATTTTAGTGCTGCCACCGTT 900 ATTATTGCAGTGCCAACTGGTATTAAGGTGTTTGCATGAATTAGCACAATGCTAGGCTCT 960 AAAGTTTCAACTCAAGCACCTATGTTGTGGTCTACTGGTTTTATTATTCTTTTTACAACA 1020 GGGGGTCTTACAGGACTTATTCTATCAAGAGCTTCAGTAGATGTTACGCTTCACGACACT 1080 TATTTTGTAACTGGTCATTTTCACTACGTCTTATCAATGGGTGCGGTGTTTACAATTTTA 1140 GCTGGGTTTACTCACTGATTTCCTCTTGTTGCTAAGGTTATAATGCATCGGCAAAAAATG 1200 AAAAGTCATTTTTTAGCAATGTTTTTAGGTGTTAATGCAGCATTTTTGCCACATCATTTT 1260 TTGGGTTTGGCTGGTATACCACGTCGAGTAGTTGATTATCCAGATCATTTTTGATTTTGA 1320 AATAAAGTATCCACATTTGGCTCTCATTTGAGTACTGGCTCATTGTTATTTTTTGTGTTT 1380 TTGTTATGAGAGTCATTTATTGCTCAACGGCCAGTTATTTCAGTGCGAAACACTTCTAGG 1440 TCCCCCGAATGGGCTGTTGTGTCTAGCCTCCCTAAGCATGCAGGGGATGAATTAGCAAAA 1500 ATGGCTAAGCTTTGTTAG KEYS (in order of precedence): >>>>>> left primer <<<<<< right primer ADDITIONAL OLIGOS start len tm gc% any_th 3'_th hairpin seq 1 LEFT PRIMER 205 18 57.89 61.11 0.00 0.00 34.59 GGGGGTTTGGTAACTGGC RIGHT PRIMER 352 18 59.88 61.11 0.00 0.00 0.00 CCTGCCCCAACTCCGTTT PRODUCT SIZE: 148, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00 2 LEFT PRIMER 205 20 60.18 55.00 0.00 0.00 34.59 GGGGGTTTGGTAACTGGCTT RIGHT PRIMER 352 18 59.88 61.11 0.00 0.00 0.00 CCTGCCCCAACTCCGTTT PRODUCT SIZE: 148, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00 3 LEFT PRIMER 419 18 57.49 55.56 0.00 0.00 42.93 AAGCCTTCACCTTGCTGG RIGHT PRIMER 503 18 60.12 61.11 0.00 0.00 0.00 GCCCCCAACAGATCGCAT PRODUCT SIZE: 85, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00 4 LEFT PRIMER 342 18 59.80 61.11 0.00 0.00 0.00 GTTGGGGCAGGGTGAACA RIGHT PRIMER 436 18 57.49 55.56 0.00 0.00 35.27 CCAGCAAGGTGAAGGCTT PRODUCT SIZE: 95, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00 Statistics con too in in not no tm tm high high high high sid many tar excl ok bad GC too too any_th 3'_th hair- poly end ered Ns get reg reg GC% clamp low high compl compl pin X stab ok libprimer3 release 2.4.0