PRIMER PICKING RESULTS FOR TRINITY_DN51983_c0_g1_i8.p1.cds No mispriming library specified Using 0-based sequence positions OLIGO start len tm gc% any_th 3'_th hairpin seq LEFT PRIMER 1347 18 59.89 55.56 9.11 0.13 42.06 TTACGCCACGGCAACTGT RIGHT PRIMER 1471 18 60.05 61.11 10.11 0.00 0.00 CGCAGTGCCAACAAGCTG SEQUENCE SIZE: 14484 INCLUDED REGION SIZE: 14484 PRODUCT SIZE: 125, PAIR ANY_TH COMPL: 10.66, PAIR 3'_TH COMPL: 0.00 0 ATGGAGCGATTGGTTTTAGCGCTAATCGTTTTGGCGGTCTGTGTCCACGCAGGTCCGATA 60 CAGAAAATCTCAGTTGATACCTGTGCAAGAACTTGTACAGGCAACAGCAAGTTTAATTAT 120 GTTGCTGGTAAGACTTACCAGTACAAGTATGATGCCGACATCAAGACTGGGGTCCAGGGA 180 GCATCAGAAGATAATGCGAAAATACACATGTCGGCAACAGTCGAGATGGAGGTCATATCA 240 AAGTGTGACCTTGTCATGCGGCTGAATGATGTCACATTGACAGAATATGACCCCATTGAC 300 TTGATAATGAAGGAAACGTCATCTGAATTCCGCAGTGGTCTTGAGAAGGCTCCCATCCGA 360 ATCTCTTTCCAAGATGGAAACATAGAGGAACTTTGTCTGAAACGAGAGGAGCCAAATTGG 420 GTGCTGAATGCCAAACGTGGAATCCTGTCAATGTTCCAGAACAACATGGACGATTTCTCA 480 ACCAATAAAACAGTGTCTGAGACTGATGTGTCTGGTGTGTGTGATACAGAGTACCACGTG 540 AGTAAATCTGGCTGGTACAAGACCACAGTCAGCAAGTCTAAGAATCTCCTAGGTTGTACT 600 GACAGGACCGGGTACAACACTGCCGTCCAGGGAGTGCCATATACCGCAGCTTCTGGCATC 660 CAGTCACTGCCAGTTGTGAAGAGCAACCATAACTGTGAACATGAGATTGACATTAAGTCG 720 CACATTCTCCATTCGGCAACTTGTAATGAACAGCACATGTTCCTGCCATTCTCACGCTCA 780 GACAGTGGAGCTGTAACATACAACACTCAGACTCTCAAGTTCGTGAAAGTAACAACTGGA 840 ATCAAGTCACCATTTGATACCAGTACTCGCAAATCAATGAAATTCGATCACATCAATGAT 900 GCAGGCAGAGAAGCAAAATCGAGGAAAGATATTACACGTAAGCTTATTGAAATCTGTGAG 960 AAGACGAAGTCTGGTGTTCGCCCTGAAACCCCACGTCTGTTCACAGACCTCGTGTTTATG 1020 ATGAAGACTGTTGATTCTGACACTCTAGCAGACACGTACAAACTGCTCCAAGAAGGTGCC 1080 ATCTGCACTGACAATAAAGAAAGAACCAGGAAGTTTTTCCTTGATGCCTTACCGATTGCT 1140 GGTTCCAGTTCCTCACTGCACTTGATGACCCAACTCTTGACCACAAAGGCTGTGACTGGA 1200 ATTGAGGCAGACATGTGGCTGTCGGCCATCTCTTTTCTCAAGAACCCAACTAAAGACATG 1260 CTGAAGGAAGTCAAGCCTTTGATCAACATGAAGGACACAACTGACAAGGCCCTGCTTTCT 1320 GTCGGTACCCTGATCCACAGTTATTGTTTACGCCACGGCAACTGTGAACGTGATGACATG >>>>>>>>>>>>>>>>>> 1380 GTGAAGGCTGTCATCTCCGCAATGCAGAACAAGATCGCTAAAGGATGCAAGATCAAGCCA 1440 GACAACTTCAAAACCAGCTTGTTGGCACTGCGAGGCATCGGCAATGCCGGATATGCCGTA <<<<<<<<<<<<<<<<<< 1500 TCAGCAATCCCCACCCTGGAGATTTGTGTGAAGATCACCTCGAACCCCATCGAAATTCGT 1560 CTGTCCGCTATTGAGGCTTTCAGGAGGATGCCATGTGATGCAAGCAGAAAAGCTTTGGTA 1620 AAGACATTCTTACAGAAGGAGGAAGATTCCGAAGTGAGGATCGCAGCATACAGGGTCTTG 1680 ATGGAATGTCCATCACCTCAGTTCCTCAGCCTAGTCCGCTCCACGTTAGAGAGTGAGGAA 1740 ATCAATCAAGTTGGGTCTTACATCTGGTCGCATCTTACAAACCTGATGGAGACATCTGAC 1800 AGACATAAGCAGGACATCAGAGCAATCCTTGAGGATGAAACATTTAAGAAGGAATTTGAC 1860 CTTGACAAAAGGAAGTTCTCACGTAACTACGAAGGTTCCATGTTTATTGAGAAACTCAAT 1920 GCCGGCGCGAAAGTTGAAGGTGATCTTGTCTGGTCATCAAGATCTTTTGTTCCAAGATCG 1980 GCCATGATGAATTTCACTGTTGATCTTTTTGGACATTCTATAAATCTTCTGGAATTCGGT 2040 GGTCGAGCTGAAGGAATAGAATACTTCTTGGAATCATTCTTTGGACCCAATGGCTACTTC 2100 AGTGGACAGGACACAAAGGGTGGAAATGATATGTTGGCCAATGGAATCAAACAGGAGAAA 2160 ATGAAGAAGATTGATAACAGGTACGGCAGCAAGATGGACGAGCTCCGTGGCTCTATGTAC 2220 ATGAGGGTGTTTGGCAATGAATTGCGTTACAAGAGCTTCCAGGGAGTGGAGGATCTGATG 2280 TCTGGTCCTAACTTCAACCTGTTCGATATGCTGATACAGCTGGCCAAGGACAACGATTAT 2340 ACTTTCAGCCATTCTACTATGTTCCTGGACACCAGCATCATTATCCCAACTGGAGCAGGT 2400 TTCCCAATGAATCTAACAGTCAACGGAACTGCAACAATTGATCTGAGAATGAAGGGAAAG 2460 ATGGATCTTAGGTCCCCGCCAGCTGTCAACATAGCAGGACTTGTACAACCAAGTGCGGCT 2520 ATCGACATATCATCAATGATGAGCGTGGATGCATTTGTAACCAAGTCCGGAATTAAGATG 2580 GTGTCCACCCTACACTCCAGTACAGCTGTCCAGGGCAAGTTCCAGGTGAACGAGGCTGGT 2640 GTGATAAGCGCAGAATACGAGATGCCACAACCCAAGATGGAGATCATTGATGTCAAGTCA 2700 TCATTCTTCACTGTTCATCGAGACATGGAGAAGGAACAAAAAATGATTGCTGATAACATT 2760 CATACAAAGAAATTCTGCTCCCCAGAGAAACTTGTCACCATCACAGGACTTGAACTATGC 2820 GCAGAGGTTACATACCCGAATGCATCTCTGAAATCAGATGCCCCATACTTCCCCCTTACA 2880 GGCCCAGTCACTGCTGGTGTCTATCTCTACAACAGAGATACCCACAAGAAATACAAGATG 2940 GAGGCCAGATCCACACATAACAAGGCAAGCAACATCCTGCATTTTACCTTTGACACACCA 3000 GAATCTCGCATCGACAGACATATTGTCCTAGATCTCAACCTCAACAAACGAGCAACAACC 3060 ATTGATATGACTTTGAACTCTCCATGGAAGAAGGCAAATCTGCAAGGTGCTATTACAAAT 3120 GACAAAGGTATCATGGGTATTAACAGCAAGATGTCGGTGGATGGCAAATCTGGTTTCTCG 3180 GTCAACTCCAAGTTTGTGAAATCCCGTGTCGGTAACAGCATCAAGTATGTTCCTTCTCTG 3240 GAAATCAGTATACCGCAGATGAAGCCCGTGAGCATCCGAGGCATGTTGATGCATAATGGT 3300 TACCGGCAGCTGGATTTGGAATATGACATGTCCGGACTGACTGCCGCACCAGTCTCCGCT 3360 CAGTTGACCATTAACAACAAACCGGCCCTAAAAGGCGTACGAGGAAGTTTGTCTTTGCAA 3420 AAGGACAAGGCCTACTCATTTGACACAAGGGTCATGATTTCATCTGACAACAACAAGATT 3480 GGATATAAGCCATTTGTCTCCCTCCGTTCTCCAACAGGGGAGGTCCTTGCATTTGGCGGA 3540 TCTGCTAATATTATCTTCAACAAGAAGGTTGTTGTAGACCTTGTCCTTGATAAGGCTTTT 3600 GCCAAAGCAGTAACACTCAAAGGTTTCGTAACCAAGACAACGAAGAACCGAGGTCGTGTT 3660 ATATACAACACTAAGATGACTTATACATCCAGTCCAGTGGATCTTCTGCTGAAAGCCAAG 3720 ATAGACAACAGAAACTCTCGTGCAGTTGCGACAGTTGTAGACTTCACCTACGTCATTCGC 3780 AAAATTGCCCGTAACAACATCAAACTTGTGTCAAAGGTCACGAACCTCAGCAGTAAATAC 3840 CTTACCAAGGCCAAAGCTTCAGCGAATGTACTAGTAAAAAGGAATCCTGAGTTGAACTTG 3900 AAATTTATGGGTAATCTAGAGCACAACAAGAAACACTCCGAGTTTGACGTCGATGTTCGC 3960 TATGGTCGTGACTTCAAAGATGAAAGCAAACATATCGACATGAGTTTTGAGATGAATAGA 4020 AAGTTCAAGGACATTACGGCAGCATCTGCCAATCTCCAGACAAGATTAACATTCCCTGGA 4080 CAGGGTCTTAATATAATCGTAAAGGGTAATCACAAACACAGCAGTAAGGAACTTGATTCC 4140 AACATCTTCCTGCGTCTTGGTAAAGGACAACCTATTGAGAGCAGTTTGCTCATACAGGAC 4200 AAAACAGAACAATTGGTGAAGGTCGTCAGTGAATACAAGCTTAGTTATCCAGGCAGGGAA 4260 GTCATCTTCAATCATACATTAGCACAGACAGATAAAACCACTTTTGTTAGTTCCTTCAAA 4320 TCTCAACTGGAGAAAAATGGAAAGAATACCATTATTACAACACTGAAAACTTACCCTGAC 4380 AAGGAAAGGGTTTCCATCTCATCAGATTTGAAACTAAGCAAACAAACACCAGTCCATTTG 4440 CAAGGAGACTTTGATTTCAATCCAGCAGACTTTATCACCAGTATGTCATATGAGAAAGAT 4500 TTAAAAAAATATTCTGGATCATTGACATCTCTGACCAAGGGAACTGAGTCATCAAGCTTA 4560 ACAATTGATTTGCAACACCCAGAAAGACGCATTGTCATGGACCTTAGTGGCAAGAGCATG 4620 ATGAAGAAATACCAGGTTGAGGCAGATTTAAAGTGGAATGCAGGCAGAAATGATTCTGAA 4680 AATGTGCATATATCATCTTGGATTGAGCCACCGACAGAAGAAAAATTGAATGGCTCCATA 4740 ACCATCACTTATCCGACAAGAACACTCACTTTGAACGCCCATCAATTACTATCAGACAAA 4800 TATAACATGCATGCTGATTTTACATGGGAAACTGACAAGAAAGTTACTATGGATACAATC 4860 GTCTTATACAAAAACAACATGATGATAAACACCTTAAAGTTGACCTCTCCATTTAGTAAG 4920 ATGCGTAGACTTGACCTAGCTGTAAACCATAAAGATGACCACGAAGAATATGCAACTAAC 4980 GTTGAGGTCAAGTGGAACAAAGGTGAATCTGTAACTTCTGAGCTCATACTTAAAAAGCCC 5040 GCCAGTCTTAAAACCGTTGTTGGTAGTGTCTTCATGAAAACAAGCTTCAAGGTTCTGAAG 5100 AAGATAAGATTAGACGTAAATCATAAATTAAGTGACTCCCTGGCTTCGTCTGTGAAATTT 5160 GCATGGAACAGGCAACTCATTAATGTAGATGTAAACCTGAAAAACACAACCAAAGGTAAG 5220 AAGATGGGATTTAATGGAAATGTTGATGTTAAGACCTCCTTTGCCTACTTGAAGAAGGGA 5280 CAACTTAAAATCTCCCATGATAACAATGGTAAGACATTCAACACTTTGACGACTTTGATG 5340 AAGAACAAGAAAACATACAAAATCGACAGTAAGATAACTCACATCCCCACAGCTAATAAA 5400 TATGAAAACACTGGAGCTATTTCTATTTCTGCACCTACTGGAAAGTCTGATATAGTATGG 5460 AGTCATATGCACACTTCAGAAAAAGTCACGTCCATGTTCGCTTCATCACAGGGAAAGAAG 5520 GGTGATAAGAATATAACAGTGAGACTAAATGGAGAATTTAAAGACCTATATGCTGCCAGT 5580 CTTAGCATTCAAACACCATATAAGTCTGCTCGTGATGCCTTGCTGGAGGTTAGTATGAAG 5640 CATGATGGGTATGTTATGGTTGACAGCAAGGCTAACGTAAATGTAAATGGTGCGAAAGTT 5700 GCTGCTGCTACCATTAGCTACAACTTGCGGAGCCCTGCTACCATTACTACACTTAATATT 5760 CCTGCCCTTGATATCAACTGCAGGATTCAAGGTAATGCAACATCAGAGAAAAACTCAGAA 5820 ACTGTTATAATGGAGGTTGTTCTTACACCAGAAATTAGTATGGCCCTCTCAGCATCACAG 5880 AAATTTTTTGATGTCACTGGTAACCCTGATGCTTTTGCCCAGTCTCTCATTTCATGGAAA 5940 TCTTCCTTCCCAGGATATGAGCATGCCAAATTCGTTTACGAAATTAGTGATAACAAAGAC 6000 GAGTCCAGTGTTACAACACATACAAAACTTGAGTATAGCCAGGGTAAAGTCATAGAAGTT 6060 AAATCTGAATTAGATAAAAACAGTTACTATTCATCACTGGCTACACCTTATGAATCGTTG 6120 CCATTTATGGAAGGAAAAATCAGTTTCGTTGGCAAACCAGAATCTTTCAGAAGCTCTGCA 6180 TTTATCAAGATACTACCTGTATTGGAGAAAACGTCTGCATCTGTATCATGGAGCACCCTT 6240 GATGGATTGAAAACTGAACTGAGAGTGGATACACCATCTACCCAGTATCCTTATATTCAG 6300 GTTAACTTGGAGGCAAAGAAAACGGAAAACGGAAAAACAACCGGTGATCTTGTAGTTGAA 6360 TATCTTCCCAGAGAAACTGTCCGAGTGGAGGTGATGAGCGATATTAGTGATATTGTCAAC 6420 CTCTCTGCAAAGGTGACATCACCATTTGTAATGATTGATCTATACCACAGTGGAAACCTA 6480 CAATCATTTAAATCCAGTGCAGAGATTGAGGGGACACCTGGCAAGAAATATGGTCTCATT 6540 GTAGGCTATACAAATGGTACAACGATTGAAGGCTATGCAACTATATCTGTGCCAGGAAGA 6600 AGAGACATAAATGCAGTCTTCTCACACCAAGGATCTGCAATGAACTTTATCACTCATGCA 6660 GAAATAACACACAACAGAATGAACCAATTCACCTCTGACTTTAAATTTGCAGCAGGCAAC 6720 TCAATAACTGTGTCTGCATCCACTTCACTTAGGTCTTTGCTCCTCATATCAGAAGACAAA 6780 TATAGAGCAGCTTTTACTACGAAGGTCATCCCATTGAAAAAGATGTCTGCTCATGGAGAA 6840 TTTGTGACAACAACCATAGGAAAGTCTGAAGCAGATGTGTCTTTTGACCTGACTAAAGAT 6900 ATTGAGGGTAGTCTTACTGTTAAATCACCTTTGATTAAAAACATTGAAGCTTCTTTTAAT 6960 CATCATCAGAGTGAAAAATATATAAACAGTAGAGCAGAAATCGTCCATGACGGTAAAAAA 7020 AATATTGATGTGCAGGCCTCTCTAAATGTTGATGAAGATTCTGTTGTTGGTGAAATAGGT 7080 ATCAAATCCCCATTGTCTGATGATATCCAGGTCCTGTCTAGGTTTGATGGTGGTAAAGAC 7140 AACTTTGTTGTTCATTTTGAAGGCTCTGTCGGTGTTAACAAATCTGAAATTGATTTATCC 7200 CACAAATGGGTTGAGAGAAACAGCCAGCAGACGATAAGTGTGAGATCGACAGGGATGAAG 7260 GACGTGACAGCAGAGATAAGTAACACCGGTGATTACTATAACTTAAGATCATATGCTGAG 7320 TTAAATCATGGACTTGAAAAACATAGAATTGATTCTACATTTAAACATCTGATAGGCAAC 7380 ACTGAAGGAAGTATTTCCATATCTTCTCCCCTGGGTGATCCACTATCCACAAACTTCTTT 7440 GTAAAGGTCACTGAGGCAAATTTAGAGTCAAATGTTGAACTGAACATAGGCTCAGACAAG 7500 AGTGAAGTTCATGTTTCTGTTGGTTCAAAACCAAATCTGGAAGGAAGCATTCGTGTCAAT 7560 TCACCTTACGTCACTGATGTCTCAGCTGGATTTGATCACACTGGAGAGTTTCCAAATATT 7620 GTTTCTCACGCACAAGTGAACATAGCCAATAAAGACATTGTTGATGTTAAGTTGATGTCA 7680 CAATCCACAGAGAGGGGAATAGCTGGATCATTTGCACTGCAGACACCATTGAAAAACTAT 7740 TCAAGTATTGAGACATCTTTCTTCCACCAAGGCATGTCTAACAATTTCAGAAGTCATGGA 7800 GATTTTGCCATTAATTCTGATAAGTCTGAAATTAATCTTTTCTACAGTTCAATGTACAAG 7860 TATGAGGGTATGGTCTCCATCAAATCTCATCTCATTGATGACCTTAAGATTGGCTTTGAA 7920 CATGATGGAACAAAAACTAACTTTGACTCTCATGGGGAAATCAGTGTTGGATTTGAGAAA 7980 TATGAACTCGATGTTAATGCAAATGTAGATTCAGATGTCATATTGAGCATGATTGTAAAA 8040 TCTCCCAGAAATGAAGATATCATTGCTAGGTTTTCACACTCGGGTAATTTCTCAGATTTT 8100 AGATGCCATGCAGAACTAATGCATGATAAAGACAGCAAATGGGAGGGAGATGTCAGTTTT 8160 TCTGCTGGTGATGAAATAAGAGGAGAGATTGTTTTCAAACCATGTCATCATCTTCACAAA 8220 GTTTTCATTTTCGATTCTAATATCAAGTTTGAATTCAGTAAATACCATGCTGAAATAAAT 8280 GGAGATTTACCATTTTTAGGAGAAGTACTTCTGAATTTCAAACGCACAGGAGAACTAAAT 8340 GACTTTAACATACATGCAGATGCTAGTTTCAGAAATAAGAAATCAGATGCAGATGTCACT 8400 TTCCAGCTTGCAAAGAAATTACTCTCTTCATCTGTAAACCTTAGATTTCCACATATGGAA 8460 GATGTGTCTTTTAGTTTCAATCACAATGGAGGTTTGAAAAACTTCAACACAAAAACTGAA 8520 ATGACAATTGGACAGAATAGAACCCAAGGAGCTGTAAGGGTGAGAATCACTGGTTCATCC 8580 ATTTATTCAAACTTAGCAATTCAATCCCATTACATGGACAGGATAAATGTTAACTTCAAC 8640 CACAATGGACAATTTGGAGATTTCACCACCAGTGGAGATGTCACTATTGGCAACATGAAA 8700 AATGAAGCAGACATGGCATTCCACCTTTCTGGCAAAGTTATGTCATCATCAGCCAGTGTC 8760 AAATCGCCTTTAATGGATAGTGTCAGCATAGACTTTAACCACAATGGAGCACCAACAGAC 8820 TTTACTACCAAAGCTTCTGTGACCATAGGTGATAAAAAAAATGAAGGAGACATTACATTC 8880 CAGATTACAGAAAAGGCTGTCATCTCTTCACTTAATATCAAATCACCATACATGGACAAC 8940 ATCAATGCTAATTTCAATCACGAAGGATTTGCTACGAAATTCACTACCAAGGCTGATGTA 9000 ACAATTGGTGTTGAAAAAAGTGAAGGTGATGTTTCATTCCAGTGGTCCAACACTTCTACG 9060 ACAATCTCTGGGAACCTGAAATCGCCATCAATGGAAGATATTACTTTTAACCAGTACACC 9120 TTATCTATGCCAACAAAGTATACGTATCATCAGGATTACTCGGTTGGTGATGCAAAAACT 9180 GAAACTGATATGATGCTGGACTACACCAACCTGAAGGCCACCTTTGCTTTGACTTCGCCA 9240 AAAACTTCCCTAAATTTTGACCACTCTGGTGATATTAAGAATTTCATCTCCGCATTGGAC 9300 CTAACAACTGAAATGGGAAGATATTCTTCTGAAGCAAAATTCACACTGAATGATAATGTA 9360 GAAGCAAGCTTCAAACTCACTTCCCCTGTTGAAGGAATGGAGTCAATTTCAGCACTTTAT 9420 ACCCACAATGGCAAACCATCCAACTTCAGATGCCATGGGGAGTTATCTCTTCCAAATAAA 9480 GCTAATGTTGCTGATTTAATTATCAATGTTGAGCAACTAACAAATATGAAAGGAAGTCTT 9540 CAAATTAAATCTGAAATCATTCCAGACTTCACAGCGAAATTCTCCAACATACTCACAGAA 9600 ACATCTCTGCAGTCAATGTCTGAGATCAGTGTTGATGATGAAGTACTCTATGGTATAGCT 9660 GTCTCTTTCACCAACAATTCATTCCTTAAAGCTGAACTGGAAGTGAAAACACCAGTAACA 9720 GAATATCGTGTTATTAGGCTGTCAGGAATTAATCAAATGGACAAAGACCAGGTAAAAGCA 9780 TCTGCAGAGCTTCTTATTGGAAGTGATAGCAGTGTAATGGAACTTTCATTTTCTTCTAAA 9840 CCAAATGTTGCGGGCGTTTTCGAACTCAGATCTGTATACCTACCTCATCTTAAGGCTACC 9900 CTAGATGCTACTGGAACACCAACTAATTTTAGAGGAAACTCAGAACTTATTTTTGATGGT 9960 GAAAAGAAAGGAAGTGTTGATGCTTCTTTCAATATGGAAGGTATATTAGATGCAAATCTC 10020 AAGATGGAACTGCCCCTGGAAAATTACACAAGGATAAATGGAGACATCTCAATTAGGAGC 10080 AGTAGTGACTTACTTGACATAAAATCTGCACTGAGTATTGAAGATAGAGAGTTAGTTGAC 10140 GGCAGTGTTCGAATTACAAACCAAAATCAGCTCTCAGGAGAAATATCTCTGAAATCTATT 10200 TACATTAACCCTTTGACTGTGAGCTTTGATTTATCTGGGGATCTTAAAGACTTCAGTACA 10260 ACAGCCGACATAAGCTACAATAATGAGAAACTGGCTGCAAGTAGTATGAAATTCAATTTC 10320 AACCCAACTCCTTTGAAAACATCTGGGTTTGCATCGTTCAACAAGATGTCTTTTGCATAC 10380 AGCTTAGATAATGCCCCAAAAGATTTTAAATCCCATGCAGAGGTAAAAGTAGAAGAAAAG 10440 ATGTGGTCTCTTGATGCAACCTGCACTAGAAAGCAAGACATTGATGCTTCATTTAAGGTC 10500 ATTATTCCAGAGATTGATCCAATTTCTGGAAAATTGTCTCATAGCCAGAGACAGTTCAGA 10560 TGTGCATCTCATGTCGAGCTTAAAACAGATTCCTCAACAAAATACCAGTATGACATGCTT 10620 CTCAAGTGGAGACGTGCCTTGGAAGGAACTTTCAGTGTCAAAACACCCATCAAAGGAATG 10680 GAAATTACAAAAGTTGTCCTCAATCATGATGGCACTTTCCCAAACATCAAATCTTCAGCT 10740 GAAGTCAATTTCAACAAGACAGACATTTCAGCCGCTGTCAGTCTGATGCATGGTGATTTA 10800 ACCAAAGGAGATGTTTCCATTAAAACTCCATTTGAAGGATTGGAAGATGTCAGTGTAACA 10860 TTCTATTCTGAAGGTGATATTGAAAAATTCCAAGCAGGTGGAAAGGTTTTGTATGCTCCA 10920 GGAAAAGATATTGAGGCAGGAGTGGAACATAGTTTGTCTGACAATAAGCTGCAGTCCCGC 10980 ATCCATTTTATGTCACCCTTCACCGATGAAATCGTTGTCAGTGCTAACCATACTGGGGTT 11040 CCTATGAATTTTGTGTCAAGTTATGAATTAGCCATGGGTTCAGATAATAGAATCAAATCT 11100 GAGACATCTTTGAAAACTGGATTCCAGTATGTTCAATTCAAATCTTCTGTCACTACAACA 11160 ATGAGTGGGGAAACATTTGTACAAAAGGTTGAGCTAAAGCATGATGGCGCTATAGAGAAG 11220 TTCAAAACAGAGGCAATGGTACAAGCAATGGAGAAATTAGTAAGAGTTGATACATCCTTC 11280 CAGCTTGAACCAGTCATTGAAGGATCAGCCTCAATCATTACACCATTCTTTGAAAAGTCA 11340 GAGGAAATAAAGGTAATCTTTTCACATAGTGGTAGTACTAGTGGATTCCTCTCAACTGGA 11400 GAGTTACAGTATTCTCCAAAGAAAAAGATAAGTGGAAAAATTGATTTCCTCAATAATGGT 11460 TGGAGGAGGAGAGTTGCAACTGTCGAAGTAAGAACGCCATTTAAGGGCATGGAGGTGAAC 11520 AAGGTTACCTACCAGCATACAGGCAACTCGGACAGCTTTGATTGTGATGCAGATATTTTT 11580 GTTGCTTCCCAGCCACTCAGCGCTACTTTCAAAGGATCTAAAGCACCTTTAAGCATGGAT 11640 CTGAACATCAAAACACCATTTGAAGGATATGAGAGTTTTGGACTTAACGGGAACTTCCTA 11700 AACAACACAATGGGCAGATATAGCGGCAGAATGGAAGCCTCATGGCACCCAAATCAGGCA 11760 GTCATTTTTGAAAGTTCATTTGCTGCATTGAGAAATAAAATTGAAGGAAGTGCCTCACTA 11820 ACAACTCCATTTGTCATGCTCCAACGGTCCTCAATTGAATTCTCACACCAGGAGCTCTCT 11880 CAAAAATTCATTGAAAATCTAAAAGCAACTTTCAATGGAAGTAGAATTGTGGATGTTGAG 11940 GTTGATCACAGCCTGATGAATGAACGCAAACATGCCATTATTACAATGCGTGCTCCACGC 12000 TCCATGAAATTTGACATGAGTGGTGATTTCACTCTGGAAAGTTCAGCAGTTGATGCATCT 12060 GCCAACTGGAACACTGAAGATAGTAAAAGTAATCTGAAACTTGAGGGAGTTTATGACATA 12120 AGAACAGACAAGAGAATGAACTTCAAACTGACCCGACCTGGACAGGTGATTTCCTATTCC 12180 GGATTATTGGACAGAAGACACAGTAAATCTGACCTTGTCTGGGGTATAGGATCAACACAA 12240 AAAGCAGGCTATGAAGTCGCAATCAATGATTATGACTCCAGACTGAAACTTATACTTCCA 12300 ACACGAAGCCTAGAGCTAACCGGGTCCCATCGATCAAAGGTCACAGAAGGGTCATTTCTA 12360 TGGGATGCTGATGTGGATGAAACAAAGAAGGTTGGCTTCCGTTCTGTGATAGTGCCATCA 12420 GATGATGCTATTAAGGCCGATGTTACATTCCAGCTTCCAAGCTTAGGAAAGAGAGTACAA 12480 GTCGGTTCAGAGGTTGTGGTCAATAGAGGACGTGTAATTTTCGACGGCAAGACAGAGTTC 12540 TCTTACTCTCCTGATGAGAGAAAGAAGATTGTGTTTTCATCAAGGCTTGAGGATAAGTCC 12600 CTTGGAGCCACGAAAAACTACAGCTTCAGCATGGGTCTAAGTCATCCATACACGACTGTT 12660 GATATCAAACTGGCATCACATGTTGGCAAATCCAAGAACAGATATTCCGCTGGTATGGGG 12720 CTTGAGTACCTTAACACACACAGACAGACCAAGACCTTCCAAGTCAATGGACAGATTAAC 12780 AAACTCAGGAAAACCATCAGCTTCGAGCTGCAGAGTCCAGTCAAATCTATCAGTATTTCG 12840 GGCAGTGCTCAGACTGAAGACAAGTTCCGTGTGTCTATCCTCAACATCTATGACCAGAAG 12900 ATGCCACTGACGACTGTTTTCACCGTCGACCCTGCCACCAGATCACTTGACTTTTACATT 12960 AATTATGACCTTGAGAACCCAAGTAGTGAGTTCCACATAAATGCCAAGTATGTGAACAGT 13020 TCTGCATTGGCTGCCGAGGTGTTCCATATCGTCAACCGTGAACGAGTGACTGACATTCTG 13080 GTTACTGCCAGACTCAACAACTCTCACCTCCTTCACACAAGAGTTCACTGGAGACCGGAA 13140 ATGATCAAGGAATACGAGACTGTTCGAGAGAGGAAACTTGAGGACTACAAGAGACGCCTG 13200 GAAATTTCAGCAGTTAAAGTAGAGGCTGCTGTAATTGAGGAAACTGTTGAGAAGTTTAAG 13260 ATGATCTGGGAAGCTGTTGTAGAGGAGATTGGCCAAGACAATGCTCTAGCTATAGAGGCT 13320 GCTGTTATCCCCATGTTTGAAGATGGACTGAGGACATATGCACAAGTGCAGGGACATGTG 13380 CAGGCAAACTTCCTTCCATTTGCCTTGGATTTGGCCAACATAACACAGTACAAGTACAAC 13440 AGATTTGTACAAACATACAGAGAGTTTTCACAGGCAGTAAAGCTTCCAGAAACAATGAAG 13500 GATCTTGACTTCCTGTCGTCTGCCAGACAACCATTGGAGAAGCCCTTTAATTATGCACTG 13560 GATCTGATCAAGATCCCTGCGAAGCACCTTCCAACCCAGGTGACCGGAGCACTGAAATAC 13620 CCGGCCAAATATATGTTTTCTAATATGGATACTGCTATGTTGGAGCAGCTTAACAAGAAC 13680 TGGGAACTTGAACACCATCTAGAATCTGTCATTGAAACCATTCTTCTTGCAATTGAGGAG 13740 GAAGCTGAGGAAGGCATACTTAAATTGAAGAGTATCATTCTCAACTCGGCACAATCCCCA 13800 ATCACTGTGTACGACCCAACCCACGGGGAGATCCAGGCAGAATTTCACTCACCCATACCA 13860 CTGAAGTCTCTGGATGAGCTACCTGTTATCAATATTGAAAGATATATTAACATGGTTATG 13920 AAAACTCGCAGTAGAAAGACAGAAAATGTTCCCAGCAAGACATCAGAATGGTTGCCACCA 13980 TTTACTGGTGTTGCCAGTATTACCAGCGGTCATAAAATCACAACTTTCGACGGGTATATC 14040 TATGACCTTGATGCTGACTGCACGTTTGTGCTTGTCCGTGATTTCAGGAATGGCAACTTC 14100 AGTATTATTCTGAACAACAATGATGGGACAACTCTGTCAATCCTTTCACATGGAAAACCA 14160 ATAGAAATCAAGCAGAGCGGAGAGATCTCTGTTGCCATGGAGCCTGTCACTCTCCCATAC 14220 AAAGAAAACAACATCACAATTACATCATCAGATGACGGAATCATCAGCATTGATGGGGCT 14280 AACCACTTCAAGGTAGATTATGACCTCTCAGTCGATCACATCGATGTAGAGATCAATGGA 14340 TGGTATTACGGCAAAACTGCAGGACTTCTTGGTACCTTTGACAACGAGCCCTCAAATGAC 14400 CTTATGACCTCCTTTGGAAAAATCATCAATAATCCTAAACGTTTCGCCAGAACTTGGGAT 14460 GTTGGAAACACAAATTGCCGTTAA KEYS (in order of precedence): >>>>>> left primer <<<<<< right primer ADDITIONAL OLIGOS start len tm gc% any_th 3'_th hairpin seq 1 LEFT PRIMER 3266 18 59.89 61.11 7.91 0.00 31.82 CGTGAGCATCCGAGGCAT RIGHT PRIMER 3362 18 60.13 66.67 0.00 0.00 0.00 CTGAGCGGAGACTGGTGC PRODUCT SIZE: 97, PAIR ANY_TH COMPL: 0.78, PAIR 3'_TH COMPL: 0.78 2 LEFT PRIMER 3253 18 60.13 61.11 0.00 0.00 0.00 CGCAGATGAAGCCCGTGA RIGHT PRIMER 3362 18 60.13 66.67 0.00 0.00 0.00 CTGAGCGGAGACTGGTGC PRODUCT SIZE: 110, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00 3 LEFT PRIMER 1922 18 59.83 61.11 12.10 0.00 0.00 CGGCGCGAAAGTTGAAGG RIGHT PRIMER 2053 18 60.13 66.67 0.00 0.00 40.25 CCTTCAGCTCGACCACCG PRODUCT SIZE: 132, PAIR ANY_TH COMPL: 11.23, PAIR 3'_TH COMPL: 14.04 4 LEFT PRIMER 1921 18 59.83 61.11 12.10 0.00 0.00 CCGGCGCGAAAGTTGAAG RIGHT PRIMER 2053 18 60.13 66.67 0.00 0.00 40.25 CCTTCAGCTCGACCACCG PRODUCT SIZE: 133, PAIR ANY_TH COMPL: 5.41, PAIR 3'_TH COMPL: 9.34 Statistics con too in in not no tm tm high high high high sid many tar excl ok bad GC too too any_th 3'_th hair- poly end ered Ns get reg reg GC% clamp low high compl compl pin X stab ok libprimer3 release 2.4.0