Bismark report for: /gscratch/scrubbed/samwhite/data/O_lurida/BSseq/whole_genome_BSseq_reads/20180830_trimgalore/7_CAGATC_L001_R1_001_trimmed.fq.gz (version: v0.19.0) Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sam/data/O_lurida/oly_genome_assemblies/Olurida_v081/oly_v081_bismark_genome/ with the specified options: -q --score-min L,0,-0.6 -p 28 --reorder --ignore-quals Final Alignment report ====================== Sequences analysed in total: 10253191 Number of alignments with a unique best hit from the different alignments: 5192738 Mapping efficiency: 50.6% Sequences with no alignments under any condition: 1560465 Sequences did not map uniquely: 3499988 Sequences which were discarded because genomic sequence could not be extracted: 3775 Number of sequences with unique best (first) alignment came from the bowtie output: CT/CT: 2491190 ((converted) top strand) CT/GA: 2491304 ((converted) bottom strand) GA/CT: 102946 (complementary to (converted) top strand) GA/GA: 103523 (complementary to (converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 39971939 Total methylated C's in CpG context: 1549737 Total methylated C's in CHG context: 96817 Total methylated C's in CHH context: 1282992 Total methylated C's in Unknown context: 23981 Total unmethylated C's in CpG context: 4197648 Total unmethylated C's in CHG context: 7683059 Total unmethylated C's in CHH context: 25161686 Total unmethylated C's in Unknown context: 133210 C methylated in CpG context: 27.0% C methylated in CHG context: 1.2% C methylated in CHH context: 4.9% C methylated in Unknown context (CN or CHN): 15.3% Bismark completed in 0d 12h 40m 46s