Bismark report for: /gscratch/scrubbed/samwhite/data/O_lurida/BSseq/whole_genome_BSseq_reads/20180830_trimgalore/2_CGATGT_L001_R1_001_trimmed.fq.gz (version: v0.19.0) Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sam/data/O_lurida/oly_genome_assemblies/Olurida_v081/oly_v081_bismark_genome/ with the specified options: -q --score-min L,0,-0.6 -p 28 --reorder --ignore-quals Final Alignment report ====================== Sequences analysed in total: 11879398 Number of alignments with a unique best hit from the different alignments: 6010969 Mapping efficiency: 50.6% Sequences with no alignments under any condition: 1790583 Sequences did not map uniquely: 4077846 Sequences which were discarded because genomic sequence could not be extracted: 4344 Number of sequences with unique best (first) alignment came from the bowtie output: CT/CT: 2853263 ((converted) top strand) CT/GA: 2858218 ((converted) bottom strand) GA/CT: 147382 (complementary to (converted) top strand) GA/GA: 147762 (complementary to (converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 46571499 Total methylated C's in CpG context: 1799874 Total methylated C's in CHG context: 196436 Total methylated C's in CHH context: 1854737 Total methylated C's in Unknown context: 29004 Total unmethylated C's in CpG context: 4959661 Total unmethylated C's in CHG context: 8836010 Total unmethylated C's in CHH context: 28924781 Total unmethylated C's in Unknown context: 156484 C methylated in CpG context: 26.6% C methylated in CHG context: 2.2% C methylated in CHH context: 6.0% C methylated in Unknown context (CN or CHN): 15.6% Bismark completed in 0d 3h 22m 13s