Bismark report for: /gscratch/scrubbed/samwhite/data/O_lurida/BSseq/whole_genome_BSseq_reads/20180830_trimgalore/1_ATCACG_L001_R1_001_trimmed.fq.gz (version: v0.19.0) Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sam/data/O_lurida/oly_genome_assemblies/Olurida_v081/oly_v081_bismark_genome/ with the specified options: -q --score-min L,0,-0.6 -p 28 --reorder --ignore-quals Final Alignment report ====================== Sequences analysed in total: 9365330 Number of alignments with a unique best hit from the different alignments: 4773958 Mapping efficiency: 51.0% Sequences with no alignments under any condition: 1416833 Sequences did not map uniquely: 3174539 Sequences which were discarded because genomic sequence could not be extracted: 3533 Number of sequences with unique best (first) alignment came from the bowtie output: CT/CT: 2287651 ((converted) top strand) CT/GA: 2288003 ((converted) bottom strand) GA/CT: 96462 (complementary to (converted) top strand) GA/GA: 98309 (complementary to (converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 36942385 Total methylated C's in CpG context: 1472670 Total methylated C's in CHG context: 107086 Total methylated C's in CHH context: 1242664 Total methylated C's in Unknown context: 22732 Total unmethylated C's in CpG context: 3911749 Total unmethylated C's in CHG context: 7089964 Total unmethylated C's in CHH context: 23118252 Total unmethylated C's in Unknown context: 127509 C methylated in CpG context: 27.4% C methylated in CHG context: 1.5% C methylated in CHH context: 5.1% C methylated in Unknown context (CN or CHN): 15.1% Bismark completed in 0d 1h 29m 14s