FastQCFastQC Report
Mon 15 Oct 2018
304428_S1_L002_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename304428_S1_L002_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30285346
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCGACAACATCATGCGTGTCCTGTTAATCCTGGTCAGCCTGGCGGCTC1440330.47558644368798036No Hit
CTCGACAACATCATGCGTGTCCTGTTAATCCTGGTCAGCCTGGCGGCTCT1054540.348201404071791No Hit
CGACAACATCATGCGTGTCCTGTTAATCCTGGTCAGCCTGGCGGCTCTGG1016170.3355319103833253No Hit
ATCCTCGACAACATCATGCGTGTCCTGTTAATCCTGGTCAGCCTGGCGGC810740.26770042514950965No Hit
CATGGAGCGACGCACCCTGCTGGTTGTCCTGCTGGTGTGCAGCCTGGTGC736790.24328267539026963No Hit
GCATCCTCGACAACATCATGCGTGTCCTGTTAATCCTGGTCAGCCTGGCG611570.20193594618334557No Hit
CAACATCATGCGTGTCCTGTTAATCCTGGTCAGCCTGGCGGCTCTGGTGC605260.19985243028096822No Hit
GGCATCCTCGACAACATCATGCGTGTCCTGTTAATCCTGGTCAGCCTGGC531050.17534883042115484No Hit
TGGCATCCTCGACAACATCATGCGTGTCCTGTTAATCCTGGTCAGCCTGG472790.1561118040388246No Hit
CATGCGTGTCCTGTTAATCCTGGTCAGCCTGGCGGCTCTGGTGCACGCCA451420.14905558615708073No Hit
TGTTAATCCTGGTCAGCCTGGCGGCTCTGGTGCACGCCAACCAACATATC446420.14740462268451548No Hit
CAAGGACCTGCCATGCTACACGGACAGAATTCTGAGCAATGCCGAGAATG422800.13960547124011724No Hit
CATCATGCGTGTCCTGTTAATCCTGGTCAGCCTGGCGGCTCTGGTGCACG403780.13332520619047905No Hit
GACAACATCATGCGTGTCCTGTTAATCCTGGTCAGCCTGGCGGCTCTGGT373240.12324112130005054No Hit
CTTGGCATCCTCGACAACATCATGCGTGTCCTGTTAATCCTGGTCAGCCT361590.1193943764089735No Hit
CATGCGTGTCCTGTTAATCCTGGTCAGCCTGGCGGCTCTGGTGCACGCCG357380.11800426516507356No Hit
ATCATGCGTGTCCTGTTAATCCTGGTCAGCCTGGCGGCTCTGGTGCACGC331030.10930368766465472No Hit
TGGAGCGACGCACCCTGCTGGTTGTCCTGCTGGTGTGCAGCCTGGTGCTG320630.105869683641719No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)