FastQCFastQC Report
Mon 15 Oct 2018
304428_S1_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename304428_S1_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30285346
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTTCACAATTTACTCATGACTCAATAAAAACGTTTGAAAGCAGTGAA887930.2931879992389719No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGCGAAGATCTCGTAT671040.22157250572603662TruSeq Adapter, Index 13 (97% over 37bp)
GTGAAAGTTAGTATATAATCTTAGTACATTCTCGGCATTGCTCAGAATTC660230.21800312269835057No Hit
GTACATTCTCGGCATTGCTCAGAATTCTGTCCGTGTAGCATGGCAGGTCC552270.18235551939872174No Hit
GTCGTGTGGAGCCAATGCCGCCGCCAAGGTTAGGCTGGGAAATGAATGGC447800.14786028860294348No Hit
GTGATTTTTTTCTTTTCTTTAGAACTAACAACACAAACTCAAGAGAAAAT397490.13124829414199196No Hit
GCTTCACAATTTACTCATGACTCAATAAAAACGTTTGAAAGCAGTGAAAG393290.12986148482503718No Hit
CTTCACAATTTACTCATGACTCAATAAAAACGTTTGAAAGCAGTGAAAGT384310.12689635442831No Hit
GGGACGTTGGAAGGGTCGTGTGGAGCCAATGCCGCCGCCAAGGTTAGGCT378030.12482274430676804No Hit
CTCGGCATTGCTCAGAATTCTGTCCGTGTAGCATGGCAGGTCCTTGTTAG370850.12245196076016433No Hit
GGACGTTGGAAGGGTCGTGTGGAGCCAATGCCGCCGCCAAGGTTAGGCTG368840.12178827344419312No Hit
GGGACGTTGGATGGGTCGTGTGGAGCCAATGCCGCCGCCAAGGTTAGGCG364770.120444389177525No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGCGAAGATCGCGTAT331310.10939614161911836TruSeq Adapter, Index 13 (97% over 37bp)
CGGCATTGCTCAGAATTCTGTCCGTGTAGCATGGCAGGTCCTTGTTAGCC309050.1020460522392579No Hit
CTGGGAACCACCGGTGAATCCAGGCAGGGTCTGGACTCCTTGAAATCCAG307600.10156727283221396No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)