FastQCFastQC Report
Mon 15 Oct 2018
304428_S1_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename304428_S1_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31231444
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTTCACAATTTACTCATGACTCAATAAAAACGTTTGAAAGCAGTGAA918860.2942098994846348No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGCGAAGATCTCGTAT776070.24848995134518917TruSeq Adapter, Index 13 (97% over 37bp)
GTGAAAGTTAGTATATAATCTTAGTACATTCTCGGCATTGCTCAGAATTC695010.2225353397044338No Hit
GTACATTCTCGGCATTGCTCAGAATTCTGTCCGTGTAGCATGGCAGGTCC579410.18552136110005032No Hit
GTCGTGTGGAGCCAATGCCGCCGCCAAGGTTAGGCTGGGAAATGAATGGC464360.1486834870651514No Hit
GCTTCACAATTTACTCATGACTCAATAAAAACGTTTGAAAGCAGTGAAAG413400.13236659822709446No Hit
CTTCACAATTTACTCATGACTCAATAAAAACGTTTGAAAGCAGTGAAAGT405550.1298531057353608No Hit
GTGATTTTTTTCTTTTCTTTAGAACTAACAACACAAACTCAAGAGAAAAT402740.12895337148035807No Hit
GGGACGTTGGAAGGGTCGTGTGGAGCCAATGCCGCCGCCAAGGTTAGGCT389840.12482291885063015No Hit
GGACGTTGGAAGGGTCGTGTGGAGCCAATGCCGCCGCCAAGGTTAGGCTG383810.12289217238882712No Hit
CTCGGCATTGCTCAGAATTCTGTCCGTGTAGCATGGCAGGTCCTTGTTAG382210.1223798681866903No Hit
GGGACGTTGGATGGGTCGTGTGGAGCCAATGCCGCCGCCAAGGTTAGGCG371350.11890260341468682No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGCGAAGATCGCGTAT355780.11391724314764314TruSeq Adapter, Index 13 (97% over 37bp)
CTGGGAACCACCGGTGAATCCAGGCAGGGTCTGGACTCCTTGAAATCCAG326410.10451325913716958No Hit
CTCAATAAAAACGTTTGAAAGCAGTGAAAGTTAGTATATAATCTTAGTAC319370.10225912064776768No Hit
CGGCATTGCTCAGAATTCTGTCCGTGTAGCATGGCAGGTCCTTGTTAGCC316330.10128574266370777No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)