FastQCFastQC Report
Tue 23 May 2023
flexbarOut_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameflexbarOut_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17551962
Sequences flagged as poor quality0
Sequence length18-50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT2991351.704282404440028Illumina RNA PCR Primer (100% over 50bp)
TTCCGCCTCAGAAGAAAGATTTTCA2752761.568348883161894No Hit
GGCGAGAATTCTACCACTGAACCACCAGTGC2576281.4678017192607868No Hit
ACTTGGTAGACAGGTTCCGCACATAAAAGA1629680.9284887923070936No Hit
ACTGCTTGTCAGTGGTAACTCAGGTCTTGGA1447000.8244092597739215No Hit
GCTTGTCAGTGGTAACTCAGGTCTTGGA1429700.8145528118167075No Hit
GGCGAGAATTCTACCACTGAACCACAGTGC1173990.6688653952190644No Hit
AGCGAGAATTCTACCACTGAACCACCAGTGC1119000.6375355644001508No Hit
TTCCGCCCCAGAAGAAAGATTTTCA1092300.622323589807225No Hit
TTCCGTCTCAGAAGAAAGATTTTCA870040.49569387171644974No Hit
AGAATTCTACCACTGAACCACCAGTGC864050.49228114782837384No Hit
AGGCGAGAATTCTACCACTGAACCACCAGTGC836780.47674442321604843No Hit
AGAATCATACCACTAGACCAAACAGCC738670.42084753829799765No Hit
AAGAGGTCAATGTGCGGGGAGGGGTG718880.4095724455191961No Hit
TGCGAGAATTCTACCACTGAACCACCAGTGC654740.37302952228360564No Hit
AGCGAGAATTCTACCACTGAACCACAGTGC536460.3056410445738203No Hit
TGGCTCCCCGGCGGGGAATCGAACCCCGGTCTCCCGC523710.2983768994030411No Hit
CTGCTTGTCAGTGGTAACTCAGGTCTTGGA492310.2804871614922594No Hit
TTTTTTTGGCCTTTAACCTACAGCATTAATA484310.27592926648314303No Hit
GAGAATTCTACCACTGAACCACCAGTGC478860.2728242005081825No Hit
AGGGACTCTCGGACAGCCTTGCTCGCCTCGCAAGAGACGAGCGCCATTTG454360.25886564704276366No Hit
ACTTGTCAGTGGTAACTCAGGTCTTGGA436940.24894083066041278No Hit
AGTGGATAACCCTCCCCAAGTCCGGACCTC432200.24624027786751135No Hit
GAGGGACTCTCGGACAGCCTTGCTCGCCTCGCAAGAGACGAGCGCCATTT427330.24346565928071176No Hit
AGAATTCTACCACTGAACCACAGTGC409480.23329585604162087No Hit
GCTCGTCAGTGGTAACTCAGGTCTTGGA408160.23254380336511668No Hit
AGTGAATCCATGTACGATTTTCGATGTGA393510.22419715812967236No Hit
AGGCGAGAATTCTACCACTGAACCACAGTGC391580.22309756595872304No Hit
ACAAGTTCGGATCTACGGGA381850.21755402615388522No Hit
ACTTTGTAAAGTTTACATTGGTAGTTCAGA378430.215605526037488No Hit
GGAGTCTCCAGATCAGGTCATTGTTACTA374750.21350889433329445No Hit
CCACAAGTTCGGATCTACGGGA359860.20502551224757665No Hit
ACTGCTCGTCAGTGGTAACTCAGGTCTTGGA353790.20156720940940961No Hit
TGCGAGAATTCTACCACTGAACCACAGTGC324220.18472008998196326No Hit
AATCAGAATCAAGTTTTCTTCAAGACTTTGTCATTCAGAGACTCTAGTCT308260.1756270894387761No Hit
GAGTGGATAACCCTCCCCAAGTCCGGACCTC281350.16029547010186096No Hit
GATAACCCTCCCCAAGTCCGGACCTC264500.15069540373890963No Hit
GCGAGAATTCTACCACTGAACCACCAGTGC252770.1440123901817928No Hit
TTTCATTTGTATCCTCACGGTTGACCCCT248420.14153403477058576No Hit
TGGCGAGAATTCTACCACTGAACCACCAGTGC247290.14089023210054807No Hit
GCTTGGTAGACAGGTTCCGCACATAAAAGA243370.13865686354608106No Hit
ACTGTTCTCGCTGTTATTATATTCAACCAA240220.1368621923862415No Hit
CCAGGTCAACAGTCCGGAAATCATGTCAA234100.13337540270426745No Hit
AAGCTTCAAGAGCACTTAGACCAAAACCT229510.13076031044278696No Hit
GAGAATTCTACCACTGAACCACAGTGC226180.1288630866452423No Hit
ACTCTTCATTTATCTACTCGTTAA225850.12867507347611623No Hit
ATGAGGTCAATGTTCGGGGAGGGGTG221440.12616253385234086No Hit
GCTGCTTGTCAGTGGTAACTCAGGTCTTGGA218040.12422542847346639No Hit
ACTGCTCGTCAGTAGTAACTCAGGCCTTGGA212170.12088107301052725No Hit
AGAGTGGATAACCCTCCCCAAGTCCGGACCTC201510.1148076779108797No Hit
ACTCGTCGTATGTCCCTTAAATTCCATAA188420.10734982220221306No Hit
AAGAGGTCAATGTGCGGGGAGGGGT185290.1055665457798963No Hit
ACTTGGTCGAGAGGTTCCACTCATAAAAGA179350.10218230873562739No Hit
GGGCGAGAATTCTACCACTGAACCACCAGTGC179230.10211394031049063No Hit
ACTTGGTTGACAGGATCCGCACATAAAAGA178260.1015612955406353No Hit
AAGAGTGGATAACCCTCCCCAAGTCCGGACCTC177850.10132770342141807No Hit
TGGATAACCCTCCCCAAGTCCGGACCTC177280.10100295340201852No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)