FastQCFastQC Report
Tue 23 May 2023
flexbarOut_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameflexbarOut_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17551962
Sequences flagged as poor quality0
Sequence length18-50
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATCTTTCTTCTGAGGCGGAA2764531.5750546861940562No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC2632721.4999576685501028No Hit
TCTTTTATGTGCGGAACCTGTCTACCAAGT1628740.9279532396435225No Hit
TCCAAGACCTGAGTTACCACTGACAAGC1455770.8294058521776653No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAGT1447050.8244377466177285No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCC1197700.682373856552333No Hit
GAGACTCTGCACGGTGGATCTCTCGGCTCGCGCATCGATGAAGAACGCAG1168130.6655267371248867No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCT1118120.637034195949148No Hit
TGAAAATCTTTCTTCTGGGGCGGAA1097740.6254229584134241No Hit
TGAAAATCTTTCTTCTGAGACGGAA873180.49748284550752786No Hit
GCACTGGTGGTTCAGTGGTAGAATTCT866640.4937567663375752No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCT840460.478841054920242No Hit
GGCTGTTTGGTCTAGTGGTATGATTCT737550.4202094329967214No Hit
CACCCCTCCCCGCACATTGACCTCTT729230.41546922218724036No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCA653490.37231735118843123No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCGTATGCCGTC621130.3538806658765556TruSeq Adapter, Index 23 (97% over 34bp)
GCACTGTGGTTCAGTGGTAGAATTCTCGCT535400.30503712348511236No Hit
GCGGGAGACCGGGGTTCGATTCCCCGCCGGGGAGCCA530380.30217704436689186No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAG495140.2820995168517343No Hit
TATTAATGCTGTAGGTTAAAGGCCAAAAAAA486120.27696049022895564No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTC485610.27666992442212446No Hit
TCCAAGACCTGAGTTACCACTGACAAGT435690.24822865956523835No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACT433900.2472088305569486No Hit
TCCAAGACCTGAGTTACCACTGACGAGC417160.23767143525037257No Hit
GCACTGTGGTTCAGTGGTAGAATTCT409700.23342119815437157No Hit
TCACATCGAAAATCGTACATGGATTCACT396950.2261570529835924No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCCT392900.2238496186352272No Hit
TAGTAACAATGACCTGATCTGGAGACTCC382440.21789017091080756No Hit
TCCCGTAGATCCGAACTTGT381800.2175255393100783No Hit
TCTGAACTACCAATGTAAACTTTACAAAGT380400.2167279076834829No Hit
TCCCGTAGATCCGAACTTGTGG361200.20578895966160363No Hit
TCCAAGACCTGAGTTACCACTGACGAGCAGT355270.20241041998609613No Hit
TTTTATGATGAATCTTTTAGACTAGAGTCTCTGAATGACAAAGTCTTGAA331860.18907287971566938No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCA323070.18406489257440278No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCGCGTATGCCGTC312580.17808835274369897TruSeq Adapter, Index 23 (97% over 34bp)
GAGGTCCGGACTTGGGGAGGGTTATCCACTC285350.16257441760641916No Hit
GAGGTCCGGACTTGGGGAGGGTTATC268380.15290598281833107No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGC259640.14792648252087146No Hit
AGGGGTCAACCGTGAGGATACAAATGAAA250520.1427304822104788No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCA249280.14202400848406577No Hit
TCTTTTATGTGCGGAACCTGTCTACCAAGC245210.1397051793981778No Hit
TTGGTTGAATATAATAACAGCGAGAACAGT240170.13683370554243451No Hit
TTGACATGATTTCCGGACTGTTGACCTGG236320.13464021856929728No Hit
AGGTTTTGGTCTAAGTGCTCTTGAAGCTT229500.13075461307402558No Hit
GCACTGTGGTTCAGTGGTAGAATTCTC229040.1304925341110014No Hit
CACCCCTCCCCGAACATTGACCTCAT226420.12899982349551575No Hit
TTAACGAGTAGATAAATGAAGAGT225730.1286067050509795No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAGC220550.12565546803257666No Hit
AGACTCTGCACGGTGGATCTCTCGGCTCGCGCATCGATGAAGAACGCAGC216480.1233366389466887No Hit
TCCAAGGCCTGAGTTACTACTGACGAGCAGT212940.12131977040515471No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTCT202240.11522358583046156No Hit
TTATGGAATTTAAGGGACATACGACGAGT188450.10736691430849725No Hit
ACCCCTCCCCGCACATTGACCTCTT188180.10721308535193957No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCC183220.10438719044628741No Hit
TCTTTTATGAGTGGAACCTCTCGACCAAGT179560.1023019534796167No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCA178170.10151001922178272No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTCTT178140.10149292711549854No Hit
TCTTTTATGTGCGGATCCTGTCAACCAAGT178060.10144734816540737No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)