diamond v2.1.1.155 (C) Max Planck Society for the Advancement of Science Documentation, support and updates available at http://www.diamondsearch.org Please cite: http://dx.doi.org/10.1038/s41592-021-01101-x Nature Methods (2021) #CPU threads: 28 Scoring parameters: (Matrix=BLOSUM62 Lambda=0.267 K=0.041 Penalties=11/1) Temporary directory: #Target sequences to report alignments for: 1 Opening the database... [0.434s] Database: /gscratch/srlab/blastdbs/20230727-uniprot-swissprot-reviewed/uniprot_sprot.dmnd (type: Diamond database, sequences: 569793, letters: 206004162) Block size = 15000000000 Opening the input file... [0.326s] Opening the output file... [0s] Loading query sequences... [1.765s] Masking queries... [0.727s] Algorithm: Double-indexed Building query histograms... [1.379s] Loading reference sequences... [0.551s] Masking reference... [0.541s] Initializing temporary storage... [0.019s] Building reference histograms... [1.612s] Allocating buffers... [0.003s] Processing query block 1, reference block 1/1, shape 1/16, index chunk 1/4. Building reference seed array... [0.164s] Building query seed array... [0.174s] Computing hash join... [0.098s] Masking low complexity seeds... [0.022s] Searching alignments... [0.468s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 1/16, index chunk 2/4. Building reference seed array... [0.199s] Building query seed array... [0.166s] Computing hash join... [0.114s] Masking low complexity seeds... [0.024s] Searching alignments... [0.421s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 1/16, index chunk 3/4. Building reference seed array... [0.213s] Building query seed array... [0.163s] Computing hash join... [0.101s] Masking low complexity seeds... [0.023s] Searching alignments... [0.409s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 1/16, index chunk 4/4. Building reference seed array... [0.164s] Building query seed array... [0.136s] Computing hash join... [0.108s] Masking low complexity seeds... [0.023s] Searching alignments... [0.412s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 2/16, index chunk 1/4. Building reference seed array... [0.161s] Building query seed array... [0.137s] Computing hash join... [0.104s] Masking low complexity seeds... [0.023s] Searching alignments... [0.381s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 2/16, index chunk 2/4. Building reference seed array... [0.212s] Building query seed array... [0.158s] Computing hash join... [0.1s] Masking low complexity seeds... [0.023s] Searching alignments... [0.384s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 2/16, index chunk 3/4. Building reference seed array... [0.209s] Building query seed array... [0.168s] Computing hash join... [0.106s] Masking low complexity seeds... [0.024s] Searching alignments... [0.386s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 2/16, index chunk 4/4. Building reference seed array... [0.165s] Building query seed array... [0.127s] Computing hash join... [0.106s] Masking low complexity seeds... [0.023s] Searching alignments... [0.374s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 3/16, index chunk 1/4. Building reference seed array... [0.164s] Building query seed array... [0.159s] Computing hash join... [0.11s] Masking low complexity seeds... [0.024s] Searching alignments... [0.428s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 3/16, index chunk 2/4. Building reference seed array... [0.201s] Building query seed array... [0.162s] Computing hash join... [0.109s] Masking low complexity seeds... [0.024s] Searching alignments... [0.428s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 3/16, index chunk 3/4. Building reference seed array... [0.227s] Building query seed array... [0.174s] Computing hash join... [0.108s] Masking low complexity seeds... [0.024s] Searching alignments... [0.433s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 3/16, index chunk 4/4. Building reference seed array... [0.169s] Building query seed array... [0.142s] Computing hash join... [0.116s] Masking low complexity seeds... [0.023s] Searching alignments... [0.411s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 4/16, index chunk 1/4. Building reference seed array... [0.162s] Building query seed array... [0.141s] Computing hash join... [0.104s] Masking low complexity seeds... [0.024s] Searching alignments... [0.431s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 4/16, index chunk 2/4. Building reference seed array... [0.228s] Building query seed array... [0.196s] Computing hash join... [0.108s] Masking low complexity seeds... [0.023s] Searching alignments... [0.416s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 4/16, index chunk 3/4. Building reference seed array... [0.214s] Building query seed array... [0.172s] Computing hash join... [0.109s] Masking low complexity seeds... [0.024s] Searching alignments... [0.412s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 4/16, index chunk 4/4. Building reference seed array... [0.165s] Building query seed array... [0.136s] Computing hash join... [0.107s] Masking low complexity seeds... [0.023s] Searching alignments... [0.394s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 5/16, index chunk 1/4. Building reference seed array... [0.162s] Building query seed array... [0.137s] Computing hash join... [0.105s] Masking low complexity seeds... [0.023s] Searching alignments... [0.396s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 5/16, index chunk 2/4. Building reference seed array... [0.197s] Building query seed array... [0.164s] Computing hash join... [0.106s] Masking low complexity seeds... [0.022s] Searching alignments... [0.393s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 5/16, index chunk 3/4. Building reference seed array... [0.214s] Building query seed array... [0.17s] Computing hash join... [0.103s] Masking low complexity seeds... [0.023s] Searching alignments... [0.391s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 5/16, index chunk 4/4. Building reference seed array... [0.165s] Building query seed array... [0.14s] Computing hash join... [0.104s] Masking low complexity seeds... [0.023s] Searching alignments... [0.4s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 6/16, index chunk 1/4. Building reference seed array... [0.188s] Building query seed array... [0.141s] Computing hash join... [0.097s] Masking low complexity seeds... [0.024s] Searching alignments... [0.406s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 6/16, index chunk 2/4. Building reference seed array... [0.2s] Building query seed array... [0.16s] Computing hash join... [0.105s] Masking low complexity seeds... [0.023s] Searching alignments... [0.405s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 6/16, index chunk 3/4. Building reference seed array... [0.257s] Building query seed array... [0.177s] Computing hash join... [0.097s] Masking low complexity seeds... [0.029s] Searching alignments... [0.389s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 6/16, index chunk 4/4. Building reference seed array... [0.163s] Building query seed array... [0.165s] Computing hash join... [0.111s] Masking low complexity seeds... [0.023s] Searching alignments... [0.392s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 7/16, index chunk 1/4. Building reference seed array... [0.199s] Building query seed array... [0.17s] Computing hash join... [0.109s] Masking low complexity seeds... [0.023s] Searching alignments... [0.397s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 7/16, index chunk 2/4. Building reference seed array... [0.217s] Building query seed array... [0.161s] Computing hash join... [0.101s] Masking low complexity seeds... [0.024s] Searching alignments... [0.402s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 7/16, index chunk 3/4. Building reference seed array... [0.214s] Building query seed array... [0.168s] Computing hash join... [0.106s] Masking low complexity seeds... [0.024s] Searching alignments... [0.378s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 7/16, index chunk 4/4. Building reference seed array... [0.163s] Building query seed array... [0.136s] Computing hash join... [0.104s] Masking low complexity seeds... [0.024s] Searching alignments... [0.38s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 8/16, index chunk 1/4. Building reference seed array... [0.165s] Building query seed array... [0.136s] Computing hash join... [0.106s] Masking low complexity seeds... [0.025s] Searching alignments... [0.396s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 8/16, index chunk 2/4. Building reference seed array... [0.212s] Building query seed array... [0.158s] Computing hash join... [0.11s] Masking low complexity seeds... [0.024s] Searching alignments... [0.407s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 8/16, index chunk 3/4. Building reference seed array... [0.21s] Building query seed array... [0.17s] Computing hash join... [0.102s] Masking low complexity seeds... [0.023s] Searching alignments... [0.385s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 8/16, index chunk 4/4. Building reference seed array... [0.163s] Building query seed array... [0.138s] Computing hash join... [0.107s] Masking low complexity seeds... [0.023s] Searching alignments... [0.393s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 9/16, index chunk 1/4. Building reference seed array... [0.171s] Building query seed array... [0.141s] Computing hash join... [0.107s] Masking low complexity seeds... [0.023s] Searching alignments... [0.418s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 9/16, index chunk 2/4. Building reference seed array... [0.2s] Building query seed array... [0.163s] Computing hash join... [0.107s] Masking low complexity seeds... [0.023s] Searching alignments... [0.406s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 9/16, index chunk 3/4. Building reference seed array... [0.211s] Building query seed array... [0.199s] Computing hash join... [0.11s] Masking low complexity seeds... [0.023s] Searching alignments... [0.406s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 9/16, index chunk 4/4. Building reference seed array... [0.166s] Building query seed array... [0.14s] Computing hash join... [0.106s] Masking low complexity seeds... [0.024s] Searching alignments... [0.404s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 10/16, index chunk 1/4. Building reference seed array... [0.163s] Building query seed array... [0.137s] Computing hash join... [0.099s] Masking low complexity seeds... [0.023s] Searching alignments... [0.38s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 10/16, index chunk 2/4. Building reference seed array... [0.213s] Building query seed array... [0.159s] Computing hash join... [0.103s] Masking low complexity seeds... [0.023s] Searching alignments... [0.372s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 10/16, index chunk 3/4. Building reference seed array... [0.209s] Building query seed array... [0.167s] Computing hash join... [0.105s] Masking low complexity seeds... [0.023s] Searching alignments... [0.384s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 10/16, index chunk 4/4. Building reference seed array... [0.164s] Building query seed array... [0.126s] Computing hash join... [0.1s] Masking low complexity seeds... [0.023s] Searching alignments... [0.369s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 11/16, index chunk 1/4. Building reference seed array... [0.164s] Building query seed array... [0.15s] Computing hash join... [0.111s] Masking low complexity seeds... [0.023s] Searching alignments... [0.403s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 11/16, index chunk 2/4. Building reference seed array... [0.2s] Building query seed array... [0.164s] Computing hash join... [0.101s] Masking low complexity seeds... [0.025s] Searching alignments... [0.417s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 11/16, index chunk 3/4. Building reference seed array... [0.214s] Building query seed array... [0.171s] Computing hash join... [0.106s] Masking low complexity seeds... [0.023s] Searching alignments... [0.406s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 11/16, index chunk 4/4. Building reference seed array... [0.165s] Building query seed array... [0.138s] Computing hash join... [0.107s] Masking low complexity seeds... [0.023s] Searching alignments... [0.764s] Deallocating memory... [0.456s] Processing query block 1, reference block 1/1, shape 12/16, index chunk 1/4. Building reference seed array... [0.217s] Building query seed array... [0.139s] Computing hash join... [0.096s] Masking low complexity seeds... [0.024s] Searching alignments... [0.39s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 12/16, index chunk 2/4. Building reference seed array... [0.261s] Building query seed array... [0.574s] Computing hash join... [0.116s] Masking low complexity seeds... [0.025s] Searching alignments... [0.398s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 12/16, index chunk 3/4. Building reference seed array... [0.213s] Building query seed array... [0.163s] Computing hash join... [0.114s] Masking low complexity seeds... [0.023s] Searching alignments... [0.386s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 12/16, index chunk 4/4. Building reference seed array... [0.163s] Building query seed array... [0.13s] Computing hash join... [0.105s] Masking low complexity seeds... [0.023s] Searching alignments... [0.382s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 13/16, index chunk 1/4. Building reference seed array... [0.163s] Building query seed array... [0.148s] Computing hash join... [0.104s] Masking low complexity seeds... [0.022s] Searching alignments... [0.376s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 13/16, index chunk 2/4. Building reference seed array... [0.197s] Building query seed array... [0.187s] Computing hash join... [0.108s] Masking low complexity seeds... [0.024s] Searching alignments... [0.404s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 13/16, index chunk 3/4. Building reference seed array... [0.212s] Building query seed array... [0.168s] Computing hash join... [0.109s] Masking low complexity seeds... [0.025s] Searching alignments... [0.393s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 13/16, index chunk 4/4. Building reference seed array... [0.164s] Building query seed array... [0.135s] Computing hash join... [0.107s] Masking low complexity seeds... [0.024s] Searching alignments... [0.378s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 14/16, index chunk 1/4. Building reference seed array... [0.164s] Building query seed array... [0.139s] Computing hash join... [0.106s] Masking low complexity seeds... [0.024s] Searching alignments... [0.397s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 14/16, index chunk 2/4. Building reference seed array... [0.197s] Building query seed array... [0.161s] Computing hash join... [0.104s] Masking low complexity seeds... [0.023s] Searching alignments... [0.384s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 14/16, index chunk 3/4. Building reference seed array... [0.211s] Building query seed array... [0.165s] Computing hash join... [0.104s] Masking low complexity seeds... [0.024s] Searching alignments... [0.398s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 14/16, index chunk 4/4. Building reference seed array... [0.179s] Building query seed array... [0.138s] Computing hash join... [0.109s] Masking low complexity seeds... [0.023s] Searching alignments... [0.385s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 15/16, index chunk 1/4. Building reference seed array... [0.164s] Building query seed array... [0.137s] Computing hash join... [0.11s] Masking low complexity seeds... [0.024s] Searching alignments... [0.406s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 15/16, index chunk 2/4. Building reference seed array... [0.201s] Building query seed array... [0.162s] Computing hash join... [0.105s] Masking low complexity seeds... [0.023s] Searching alignments... [0.392s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 15/16, index chunk 3/4. Building reference seed array... [0.218s] Building query seed array... [0.165s] Computing hash join... [0.103s] Masking low complexity seeds... [0.023s] Searching alignments... [0.391s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 15/16, index chunk 4/4. Building reference seed array... [0.163s] Building query seed array... [0.144s] Computing hash join... [0.104s] Masking low complexity seeds... [0.023s] Searching alignments... [0.38s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 16/16, index chunk 1/4. Building reference seed array... [0.163s] Building query seed array... [0.134s] Computing hash join... [0.101s] Masking low complexity seeds... [0.023s] Searching alignments... [0.379s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 16/16, index chunk 2/4. Building reference seed array... [0.199s] Building query seed array... [0.157s] Computing hash join... [0.102s] Masking low complexity seeds... [0.023s] Searching alignments... [0.56s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 16/16, index chunk 3/4. Building reference seed array... [0.212s] Building query seed array... [0.166s] Computing hash join... [0.11s] Masking low complexity seeds... [0.023s] Searching alignments... [0.382s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 16/16, index chunk 4/4. Building reference seed array... [0.162s] Building query seed array... [0.137s] Computing hash join... [0.107s] Masking low complexity seeds... [0.024s] Searching alignments... [0.385s] Deallocating memory... [0s] Deallocating buffers... [0.003s] Clearing query masking... [0.09s] Computing alignments... Loading trace points... [1.828s] Sorting trace points... [0.216s] Computing alignments... [6.789s] Deallocating buffers... [0.003s] Loading trace points... [0s] [9.061s] Deallocating reference... [0.001s] Loading reference sequences... [0s] Deallocating buffers... [0s] Deallocating queries... [0.002s] Loading query sequences... [0s] Closing the input file... [0s] Closing the output file... [0.002s] Closing the database... [0s] Cleaning up... [0s] Total time = 73.995s Reported 39699 pairwise alignments, 39699 HSPs. 39699 queries aligned. Generating checksum for 20231109-mmag-transcriptome-diamond-blastx.sh. 8d5d09c57e0cb7915fafdc6edfdf65b9 20231109-mmag-transcriptome-diamond-blastx.sh Generating checksum for slurm-4979676.out. f0a7f065d13e2840584e277fca14462f slurm-4979676.out Generating checksum for trinity_denovo.Trinity.blastx.outfmt6. f4d66e229b23a766b35c2d0601375741 trinity_denovo.Trinity.blastx.outfmt6 Generating checksum for /gscratch/srlab/sam/data/M_magister/transcriptomes/trinity_denovo.Trinity.fasta. 081e52972c316d4e45e643e669acb749 /gscratch/srlab/sam/data/M_magister/transcriptomes/trinity_denovo.Trinity.fasta Logging program options...