Version MEGAN Community Edition (version 6.24.20, built 5 Feb 2023) Author(s) Daniel H. Huson Copyright (C) 2023 Daniel H. Huson. This program comes with ABSOLUTELY NO WARRANTY. Functional classifications to use: EC, EGGNOG, GTDB, INTERPRO2GO, SEED Loading ncbi.map: 2,396,736 Loading ncbi.tre: 2,396,740 Loading ec.map: 8,200 Loading ec.tre: 8,204 Loading eggnog.map: 30,875 Loading eggnog.tre: 30,986 Loading gtdb.map: 240,103 Loading gtdb.tre: 240,107 Loading interpro2go.map: 14,242 Loading interpro2go.tre: 28,907 Loading seed.map: 961 Loading seed.tre: 962 In DAA file: CH01-06.trimmed.R1.blastx.meganized.daa Output file: CH01-06.trimmed.R1.blastx.meganized.daa.daa2rma.rma6 Classifications: Taxonomy, SEED, EGGNOG, GTDB, EC, INTERPRO2GO Generating RMA6 file Parsing matches Annotating RMA6 file using FAST mode (accession database and first accession per line) Parsing file CH01-06.trimmed.R1.blastx.meganized.daa Parsing file: CH01-06.trimmed.R1.blastx.meganized.daa 10% 20% 30% 40% 50% 60% 70% 80% 90% 100% (4,194.7s) Total reads: 4,216,422 Alignments: 75,492,385 100% (0.0s) Binning reads: Initializing... Initializing binning... Using 'Naive LCA' algorithm for binning: Taxonomy Using Best-Hit algorithm for binning: SEED Using Best-Hit algorithm for binning: EGGNOG Using 'Naive LCA' algorithm for binning: GTDB Using Best-Hit algorithm for binning: EC Using Best-Hit algorithm for binning: INTERPRO2GO Binning reads... Binning reads: Analyzing alignments Total reads: 4,216,422 With hits: 4,216,422 Alignments: 75,492,385 Assig. Taxonomy: 2,388,550 Assig. SEED: 75,010 Assig. EGGNOG: 16,700 Assig. GTDB: 98,077 Assig. EC: 276,727 Assig. INTERPRO2GO: 1,099,158 MinSupport set to: 421 Binning reads: Applying min-support & disabled filter to Taxonomy... Min-supp. changes: 4,762 Binning reads: Applying min-support & disabled filter to GTDB... Min-supp. changes: 3,267 Binning reads: Writing classification tables Numb. Tax. classes: 308 Numb. SEED classes: 466 Numb. EGG. classes: 1,576 Numb. GTDB classes: 106 Numb. EC classes: 2,286 Numb. INT. classes: 8,405 Binning reads: Syncing Class. Taxonomy: 308 Class. SEED: 466 Class. EGGNOG: 1,576 Class. GTDB: 106 Class. EC: 2,286 Class. INTERPRO2GO: 8,405 100% (1,784.4s) In DAA file: CH01-06.trimmed.R2.blastx.meganized.daa Output file: CH01-06.trimmed.R2.blastx.meganized.daa.daa2rma.rma6 Classifications: Taxonomy, SEED, EGGNOG, GTDB, EC, INTERPRO2GO Generating RMA6 file Parsing matches Annotating RMA6 file using FAST mode (accession database and first accession per line) Parsing file CH01-06.trimmed.R2.blastx.meganized.daa Parsing file: CH01-06.trimmed.R2.blastx.meganized.daa 10% 20% 30% 40% 50% 60% 70% 80% 90% 100% (2,526.5s) Total reads: 4,240,909 Alignments: 75,272,014 100% (0.0s) Binning reads: Initializing... Initializing binning... Using 'Naive LCA' algorithm for binning: Taxonomy Using Best-Hit algorithm for binning: SEED Using Best-Hit algorithm for binning: EGGNOG Using 'Naive LCA' algorithm for binning: GTDB Using Best-Hit algorithm for binning: EC Using Best-Hit algorithm for binning: INTERPRO2GO Binning reads... Binning reads: Analyzing alignments Total reads: 4,240,909 With hits: 4,240,909 Alignments: 75,272,014 Assig. Taxonomy: 2,388,461 Assig. SEED: 74,459 Assig. EGGNOG: 16,729 Assig. GTDB: 98,706 Assig. EC: 275,366 Assig. INTERPRO2GO: 1,099,468 MinSupport set to: 424 Binning reads: Applying min-support & disabled filter to Taxonomy... Min-supp. changes: 4,724 Binning reads: Applying min-support & disabled filter to GTDB... Min-supp. changes: 3,252 Binning reads: Writing classification tables Numb. Tax. classes: 301 Numb. SEED classes: 472 Numb. EGG. classes: 1,584 Numb. GTDB classes: 107 Numb. EC classes: 2,289 Numb. INT. classes: 8,396 Binning reads: Syncing Class. Taxonomy: 301 Class. SEED: 472 Class. EGGNOG: 1,584 Class. GTDB: 107 Class. EC: 2,289 Class. INTERPRO2GO: 8,396 100% (1,700.8s) Total time: 10,217.5s Peak memory: 96.9 of 488.3G Version MEGAN Community Edition (version 6.24.20, built 5 Feb 2023) Author(s) Daniel H. Huson Copyright (C) 2023 Daniel H. Huson. This program comes with ABSOLUTELY NO WARRANTY. Functional classifications to use: EC, EGGNOG, GTDB, INTERPRO2GO, SEED Loading ncbi.map: 2,396,736 Loading ncbi.tre: 2,396,740 Loading ec.map: 8,200 Loading ec.tre: 8,204 Loading eggnog.map: 30,875 Loading eggnog.tre: 30,986 Loading gtdb.map: 240,103 Loading gtdb.tre: 240,107 Loading interpro2go.map: 14,242 Loading interpro2go.tre: 28,907 Loading seed.map: 961 Loading seed.tre: 962 In DAA file: CH01-14.trimmed.R1.blastx.meganized.daa Output file: CH01-14.trimmed.R1.blastx.meganized.daa.daa2rma.rma6 Classifications: Taxonomy, SEED, EGGNOG, GTDB, EC, INTERPRO2GO Generating RMA6 file Parsing matches Annotating RMA6 file using FAST mode (accession database and first accession per line) Parsing file CH01-14.trimmed.R1.blastx.meganized.daa Parsing file: CH01-14.trimmed.R1.blastx.meganized.daa 10% 20% 30% 40% 50% 60% 70% 80% 90% 100% (4,994.7s) Total reads: 7,672,210 Alignments: 138,386,238 100% (0.0s) Binning reads: Initializing... Initializing binning... Using 'Naive LCA' algorithm for binning: Taxonomy Using Best-Hit algorithm for binning: SEED Using Best-Hit algorithm for binning: EGGNOG Using 'Naive LCA' algorithm for binning: GTDB Using Best-Hit algorithm for binning: EC Using Best-Hit algorithm for binning: INTERPRO2GO Binning reads... Binning reads: Analyzing alignments Total reads: 7,672,210 With hits: 7,672,210 Alignments: 138,386,238 Assig. Taxonomy: 4,618,479 Assig. SEED: 139,887 Assig. EGGNOG: 35,889 Assig. GTDB: 204,079 Assig. EC: 478,017 Assig. INTERPRO2GO: 2,012,027 MinSupport set to: 767 Binning reads: Applying min-support & disabled filter to Taxonomy... Min-supp. changes: 6,909 Binning reads: Applying min-support & disabled filter to GTDB... Min-supp. changes: 5,249 Binning reads: Writing classification tables Numb. Tax. classes: 331 Numb. SEED classes: 496 Numb. EGG. classes: 1,658 Numb. GTDB classes: 103 Numb. EC classes: 2,433 Numb. INT. classes: 8,943 Binning reads: Syncing Class. Taxonomy: 331 Class. SEED: 496 Class. EGGNOG: 1,658 Class. GTDB: 103 Class. EC: 2,433 Class. INTERPRO2GO: 8,943 100% (3,214.7s) In DAA file: CH01-14.trimmed.R2.blastx.meganized.daa Output file: CH01-14.trimmed.R2.blastx.meganized.daa.daa2rma.rma6 Classifications: Taxonomy, SEED, EGGNOG, GTDB, EC, INTERPRO2GO Generating RMA6 file Parsing matches Annotating RMA6 file using FAST mode (accession database and first accession per line) Parsing file CH01-14.trimmed.R2.blastx.meganized.daa Parsing file: CH01-14.trimmed.R2.blastx.meganized.daa 10% 20% 30% 40% 50% 60% 70% 80% 90% 100% (7,258.3s) Total reads: 7,709,948 Alignments: 137,982,651 100% (0.0s) Binning reads: Initializing... Initializing binning... Using 'Naive LCA' algorithm for binning: Taxonomy Using Best-Hit algorithm for binning: SEED Using Best-Hit algorithm for binning: EGGNOG Using 'Naive LCA' algorithm for binning: GTDB Using Best-Hit algorithm for binning: EC Using Best-Hit algorithm for binning: INTERPRO2GO Binning reads... Binning reads: Analyzing alignments Total reads: 7,709,948 With hits: 7,709,948 Alignments: 137,982,651 Assig. Taxonomy: 4,617,098 Assig. SEED: 139,408 Assig. EGGNOG: 35,685 Assig. GTDB: 204,719 Assig. EC: 476,762 Assig. INTERPRO2GO: 2,009,968 MinSupport set to: 770 Binning reads: Applying min-support & disabled filter to Taxonomy... Min-supp. changes: 6,852 Binning reads: Applying min-support & disabled filter to GTDB... Min-supp. changes: 5,172 Binning reads: Writing classification tables Numb. Tax. classes: 329 Numb. SEED classes: 490 Numb. EGG. classes: 1,633 Numb. GTDB classes: 103 Numb. EC classes: 2,431 Numb. INT. classes: 8,940 Binning reads: Syncing Class. Taxonomy: 329 Class. SEED: 490 Class. EGGNOG: 1,633 Class. GTDB: 103 Class. EC: 2,431 Class. INTERPRO2GO: 8,940 100% (3,135.0s) Total time: 18,614.7s Peak memory: 102 of 488.3G Version MEGAN Community Edition (version 6.24.20, built 5 Feb 2023) Author(s) Daniel H. Huson Copyright (C) 2023 Daniel H. Huson. This program comes with ABSOLUTELY NO WARRANTY. Functional classifications to use: EC, EGGNOG, GTDB, INTERPRO2GO, SEED Loading ncbi.map: 2,396,736 Loading ncbi.tre: 2,396,740 Loading ec.map: 8,200 Loading ec.tre: 8,204 Loading eggnog.map: 30,875 Loading eggnog.tre: 30,986 Loading gtdb.map: 240,103 Loading gtdb.tre: 240,107 Loading interpro2go.map: 14,242 Loading interpro2go.tre: 28,907 Loading seed.map: 961 Loading seed.tre: 962 In DAA file: CH01-22.trimmed.R1.blastx.meganized.daa Output file: CH01-22.trimmed.R1.blastx.meganized.daa.daa2rma.rma6 Classifications: Taxonomy, SEED, EGGNOG, GTDB, EC, INTERPRO2GO Generating RMA6 file Parsing matches Annotating RMA6 file using FAST mode (accession database and first accession per line) Parsing file CH01-22.trimmed.R1.blastx.meganized.daa Parsing file: CH01-22.trimmed.R1.blastx.meganized.daa 10% 20% 30% 40% 50% 60% 70% 80% 90% 100% (4,378.3s) Total reads: 5,022,039 Alignments: 77,337,743 100% (0.0s) Binning reads: Initializing... Initializing binning... Using 'Naive LCA' algorithm for binning: Taxonomy Using Best-Hit algorithm for binning: SEED Using Best-Hit algorithm for binning: EGGNOG Using 'Naive LCA' algorithm for binning: GTDB Using Best-Hit algorithm for binning: EC Using Best-Hit algorithm for binning: INTERPRO2GO Binning reads... Binning reads: Analyzing alignments Total reads: 5,022,039 With hits: 5,022,039 Alignments: 77,337,743 Assig. Taxonomy: 2,458,140 Assig. SEED: 108,919 Assig. EGGNOG: 45,126 Assig. GTDB: 241,525 Assig. EC: 334,193 Assig. INTERPRO2GO: 1,093,058 MinSupport set to: 502 Binning reads: Applying min-support & disabled filter to Taxonomy... Min-supp. changes: 6,967 Binning reads: Applying min-support & disabled filter to GTDB... Min-supp. changes: 5,529 Binning reads: Writing classification tables Numb. Tax. classes: 469 Numb. SEED classes: 532 Numb. EGG. classes: 1,846 Numb. GTDB classes: 183 Numb. EC classes: 2,572 Numb. INT. classes: 8,914 Binning reads: Syncing Class. Taxonomy: 469 Class. SEED: 532 Class. EGGNOG: 1,846 Class. GTDB: 183 Class. EC: 2,572 Class. INTERPRO2GO: 8,914 100% (1,923.3s) In DAA file: CH01-22.trimmed.R2.blastx.meganized.daa Output file: CH01-22.trimmed.R2.blastx.meganized.daa.daa2rma.rma6 Classifications: Taxonomy, SEED, EGGNOG, GTDB, EC, INTERPRO2GO Generating RMA6 file Parsing matches Annotating RMA6 file using FAST mode (accession database and first accession per line) Parsing file CH01-22.trimmed.R2.blastx.meganized.daa Parsing file: CH01-22.trimmed.R2.blastx.meganized.daa 10% 20% 30% 40% 50% 60% 70% 80% 90% 100% (2,379.2s) Total reads: 5,072,643 Alignments: 77,237,249 100% (0.0s) Binning reads: Initializing... Initializing binning... Using 'Naive LCA' algorithm for binning: Taxonomy Using Best-Hit algorithm for binning: SEED Using Best-Hit algorithm for binning: EGGNOG Using 'Naive LCA' algorithm for binning: GTDB Using Best-Hit algorithm for binning: EC Using Best-Hit algorithm for binning: INTERPRO2GO Binning reads... Binning reads: Analyzing alignments Total reads: 5,072,643 With hits: 5,072,643 Alignments: 77,237,249 Assig. Taxonomy: 2,461,937 Assig. SEED: 109,899 Assig. EGGNOG: 45,145 Assig. GTDB: 243,902 Assig. EC: 333,206 Assig. INTERPRO2GO: 1,093,631 MinSupport set to: 507 Binning reads: Applying min-support & disabled filter to Taxonomy... Min-supp. changes: 6,921 Binning reads: Applying min-support & disabled filter to GTDB... Min-supp. changes: 5,485 Binning reads: Writing classification tables Numb. Tax. classes: 460 Numb. SEED classes: 535 Numb. EGG. classes: 1,850 Numb. GTDB classes: 180 Numb. EC classes: 2,574 Numb. INT. classes: 8,887 Binning reads: Syncing Class. Taxonomy: 460 Class. SEED: 535 Class. EGGNOG: 1,850 Class. GTDB: 180 Class. EC: 2,574 Class. INTERPRO2GO: 8,887 100% (1,829.3s) Total time: 10,518.7s Peak memory: 80.8 of 488.3G Version MEGAN Community Edition (version 6.24.20, built 5 Feb 2023) Author(s) Daniel H. Huson Copyright (C) 2023 Daniel H. Huson. This program comes with ABSOLUTELY NO WARRANTY. Functional classifications to use: EC, EGGNOG, GTDB, INTERPRO2GO, SEED Loading ncbi.map: 2,396,736 Loading ncbi.tre: 2,396,740 Loading ec.map: 8,200 Loading ec.tre: 8,204 Loading eggnog.map: 30,875 Loading eggnog.tre: 30,986 Loading gtdb.map: 240,103 Loading gtdb.tre: 240,107 Loading interpro2go.map: 14,242 Loading interpro2go.tre: 28,907 Loading seed.map: 961 Loading seed.tre: 962 In DAA file: CH01-38.trimmed.R1.blastx.meganized.daa Output file: CH01-38.trimmed.R1.blastx.meganized.daa.daa2rma.rma6 Classifications: Taxonomy, SEED, EGGNOG, GTDB, EC, INTERPRO2GO Generating RMA6 file Parsing matches Annotating RMA6 file using FAST mode (accession database and first accession per line) Parsing file CH01-38.trimmed.R1.blastx.meganized.daa Parsing file: CH01-38.trimmed.R1.blastx.meganized.daa 10% 20% 30% 40% 50% 60% 70% 80% 90% 100% (4,430.9s) Total reads: 8,073,779 Alignments: 135,332,504 100% (0.0s) Binning reads: Initializing... Initializing binning... Using 'Naive LCA' algorithm for binning: Taxonomy Using Best-Hit algorithm for binning: SEED Using Best-Hit algorithm for binning: EGGNOG Using 'Naive LCA' algorithm for binning: GTDB Using Best-Hit algorithm for binning: EC Using Best-Hit algorithm for binning: INTERPRO2GO Binning reads... Binning reads: Analyzing alignments Total reads: 8,073,779 With hits: 8,073,779 Alignments: 135,332,504 Assig. Taxonomy: 4,268,936 Assig. SEED: 131,459 Assig. EGGNOG: 46,934 Assig. GTDB: 228,732 Assig. EC: 485,570 Assig. INTERPRO2GO: 1,917,068 MinSupport set to: 807 Binning reads: Applying min-support & disabled filter to Taxonomy... Min-supp. changes: 6,325 Binning reads: Applying min-support & disabled filter to GTDB... Min-supp. changes: 4,475 Binning reads: Writing classification tables Numb. Tax. classes: 321 Numb. SEED classes: 497 Numb. EGG. classes: 1,675 Numb. GTDB classes: 104 Numb. EC classes: 2,383 Numb. INT. classes: 8,892 Binning reads: Syncing Class. Taxonomy: 321 Class. SEED: 497 Class. EGGNOG: 1,675 Class. GTDB: 104 Class. EC: 2,383 Class. INTERPRO2GO: 8,892 100% (3,097.1s) In DAA file: CH01-38.trimmed.R2.blastx.meganized.daa Output file: CH01-38.trimmed.R2.blastx.meganized.daa.daa2rma.rma6 Classifications: Taxonomy, SEED, EGGNOG, GTDB, EC, INTERPRO2GO Generating RMA6 file Parsing matches Annotating RMA6 file using FAST mode (accession database and first accession per line) Parsing file CH01-38.trimmed.R2.blastx.meganized.daa Parsing file: CH01-38.trimmed.R2.blastx.meganized.daa 10% 20% 30% 40% 50% 60% 70% 80% 90% 100% (5,044.9s) Total reads: 8,153,552 Alignments: 135,136,709 100% (0.0s) Binning reads: Initializing... Initializing binning... Using 'Naive LCA' algorithm for binning: Taxonomy Using Best-Hit algorithm for binning: SEED Using Best-Hit algorithm for binning: EGGNOG Using 'Naive LCA' algorithm for binning: GTDB Using Best-Hit algorithm for binning: EC Using Best-Hit algorithm for binning: INTERPRO2GO Binning reads... Binning reads: Analyzing alignments Total reads: 8,153,552 With hits: 8,153,552 Alignments: 135,136,709 Assig. Taxonomy: 4,270,029 Assig. SEED: 131,439 Assig. EGGNOG: 46,799 Assig. GTDB: 229,076 Assig. EC: 484,254 Assig. INTERPRO2GO: 1,916,064 MinSupport set to: 815 Binning reads: Applying min-support & disabled filter to Taxonomy... Min-supp. changes: 6,146 Binning reads: Applying min-support & disabled filter to GTDB... Min-supp. changes: 4,407 Binning reads: Writing classification tables Numb. Tax. classes: 319 Numb. SEED classes: 494 Numb. EGG. classes: 1,682 Numb. GTDB classes: 106 Numb. EC classes: 2,373 Numb. INT. classes: 8,857 Binning reads: Syncing Class. Taxonomy: 319 Class. SEED: 494 Class. EGGNOG: 1,682 Class. GTDB: 106 Class. EC: 2,373 Class. INTERPRO2GO: 8,857 100% (3,126.7s) Total time: 15,710.7s Peak memory: 92.9 of 488.3G Version MEGAN Community Edition (version 6.24.20, built 5 Feb 2023) Author(s) Daniel H. Huson Copyright (C) 2023 Daniel H. Huson. This program comes with ABSOLUTELY NO WARRANTY. Functional classifications to use: EC, EGGNOG, GTDB, INTERPRO2GO, SEED Loading ncbi.map: 2,396,736 Loading ncbi.tre: 2,396,740 Loading ec.map: 8,200 Loading ec.tre: 8,204 Loading eggnog.map: 30,875 Loading eggnog.tre: 30,986 Loading gtdb.map: 240,103 Loading gtdb.tre: 240,107 Loading interpro2go.map: 14,242 Loading interpro2go.tre: 28,907 Loading seed.map: 961 Loading seed.tre: 962 In DAA file: CH03-04.trimmed.R1.blastx.meganized.daa Output file: CH03-04.trimmed.R1.blastx.meganized.daa.daa2rma.rma6 Classifications: Taxonomy, SEED, EGGNOG, GTDB, EC, INTERPRO2GO Generating RMA6 file Parsing matches Annotating RMA6 file using FAST mode (accession database and first accession per line) Parsing file CH03-04.trimmed.R1.blastx.meganized.daa Parsing file: CH03-04.trimmed.R1.blastx.meganized.daa 10% 20% 30% 40% 50% 60% 70% 80% 90% 100% (3,243.2s) Total reads: 4,276,280 Alignments: 72,629,208 100% (0.0s) Binning reads: Initializing... Initializing binning... Using 'Naive LCA' algorithm for binning: Taxonomy Using Best-Hit algorithm for binning: SEED Using Best-Hit algorithm for binning: EGGNOG Using 'Naive LCA' algorithm for binning: GTDB Using Best-Hit algorithm for binning: EC Using Best-Hit algorithm for binning: INTERPRO2GO Binning reads... Binning reads: Analyzing alignments Total reads: 4,276,280 With hits: 4,276,280 Alignments: 72,629,208 Assig. Taxonomy: 2,303,073 Assig. SEED: 74,613 Assig. EGGNOG: 19,499 Assig. GTDB: 109,298 Assig. EC: 272,377 Assig. INTERPRO2GO: 1,015,870 MinSupport set to: 427 Binning reads: Applying min-support & disabled filter to Taxonomy... Min-supp. changes: 5,152 Binning reads: Applying min-support & disabled filter to GTDB... Min-supp. changes: 3,606 Binning reads: Writing classification tables Numb. Tax. classes: 345 Numb. SEED classes: 484 Numb. EGG. classes: 1,570 Numb. GTDB classes: 112 Numb. EC classes: 2,306 Numb. INT. classes: 8,604 Binning reads: Syncing Class. Taxonomy: 345 Class. SEED: 484 Class. EGGNOG: 1,570 Class. GTDB: 112 Class. EC: 2,306 Class. INTERPRO2GO: 8,604 100% (1,791.7s) In DAA file: CH03-04.trimmed.R2.blastx.meganized.daa Output file: CH03-04.trimmed.R2.blastx.meganized.daa.daa2rma.rma6 Classifications: Taxonomy, SEED, EGGNOG, GTDB, EC, INTERPRO2GO Generating RMA6 file Parsing matches Annotating RMA6 file using FAST mode (accession database and first accession per line) Parsing file CH03-04.trimmed.R2.blastx.meganized.daa Parsing file: CH03-04.trimmed.R2.blastx.meganized.daa 10% 20% 30% 40% 50% 60% 70% 80% 90% 100% (2,242.2s) Total reads: 4,301,510 Alignments: 72,632,563 100% (0.0s) Binning reads: Initializing... Initializing binning... Using 'Naive LCA' algorithm for binning: Taxonomy Using Best-Hit algorithm for binning: SEED Using Best-Hit algorithm for binning: EGGNOG Using 'Naive LCA' algorithm for binning: GTDB Using Best-Hit algorithm for binning: EC Using Best-Hit algorithm for binning: INTERPRO2GO Binning reads... Binning reads: Analyzing alignments Total reads: 4,301,510 With hits: 4,301,510 Alignments: 72,632,563 Assig. Taxonomy: 2,305,967 Assig. SEED: 74,893 Assig. EGGNOG: 19,917 Assig. GTDB: 110,014 Assig. EC: 271,626 Assig. INTERPRO2GO: 1,016,138 MinSupport set to: 430 Binning reads: Applying min-support & disabled filter to Taxonomy... Min-supp. changes: 5,062 Binning reads: Applying min-support & disabled filter to GTDB... Min-supp. changes: 3,642 Binning reads: Writing classification tables Numb. Tax. classes: 342 Numb. SEED classes: 479 Numb. EGG. classes: 1,595 Numb. GTDB classes: 111 Numb. EC classes: 2,323 Numb. INT. classes: 8,571 Binning reads: Syncing Class. Taxonomy: 342 Class. SEED: 479 Class. EGGNOG: 1,595 Class. GTDB: 111 Class. EC: 2,323 Class. INTERPRO2GO: 8,571 100% (1,668.2s) Total time: 8,953.6s Peak memory: 93.5 of 488.3G Version MEGAN Community Edition (version 6.24.20, built 5 Feb 2023) Author(s) Daniel H. Huson Copyright (C) 2023 Daniel H. Huson. This program comes with ABSOLUTELY NO WARRANTY. Functional classifications to use: EC, EGGNOG, GTDB, INTERPRO2GO, SEED Loading ncbi.map: 2,396,736 Loading ncbi.tre: 2,396,740 Loading ec.map: 8,200 Loading ec.tre: 8,204 Loading eggnog.map: 30,875 Loading eggnog.tre: 30,986 Loading gtdb.map: 240,103 Loading gtdb.tre: 240,107 Loading interpro2go.map: 14,242 Loading interpro2go.tre: 28,907 Loading seed.map: 961 Loading seed.tre: 962 In DAA file: CH03-15.trimmed.R1.blastx.meganized.daa Output file: CH03-15.trimmed.R1.blastx.meganized.daa.daa2rma.rma6 Classifications: Taxonomy, SEED, EGGNOG, GTDB, EC, INTERPRO2GO Generating RMA6 file Parsing matches Annotating RMA6 file using FAST mode (accession database and first accession per line) Parsing file CH03-15.trimmed.R1.blastx.meganized.daa Parsing file: CH03-15.trimmed.R1.blastx.meganized.daa 10% 20% 30% 40% 50% 60% 70% 80% 90% 100% (3,460.9s) Total reads: 5,364,441 Alignments: 72,642,848 100% (0.0s) Binning reads: Initializing... Initializing binning... Using 'Naive LCA' algorithm for binning: Taxonomy Using Best-Hit algorithm for binning: SEED Using Best-Hit algorithm for binning: EGGNOG Using 'Naive LCA' algorithm for binning: GTDB Using Best-Hit algorithm for binning: EC Using Best-Hit algorithm for binning: INTERPRO2GO Binning reads... Binning reads: Analyzing alignments Total reads: 5,364,441 With hits: 5,364,441 Alignments: 72,642,848 Assig. Taxonomy: 2,125,760 Assig. SEED: 83,993 Assig. EGGNOG: 23,446 Assig. GTDB: 170,737 Assig. EC: 296,632 Assig. INTERPRO2GO: 948,196 MinSupport set to: 536 Binning reads: Applying min-support & disabled filter to Taxonomy... Min-supp. changes: 5,974 Binning reads: Applying min-support & disabled filter to GTDB... Min-supp. changes: 4,561 Binning reads: Writing classification tables Numb. Tax. classes: 325 Numb. SEED classes: 504 Numb. EGG. classes: 1,629 Numb. GTDB classes: 115 Numb. EC classes: 2,441 Numb. INT. classes: 8,756 Binning reads: Syncing Class. Taxonomy: 325 Class. SEED: 504 Class. EGGNOG: 1,629 Class. GTDB: 115 Class. EC: 2,441 Class. INTERPRO2GO: 8,756 100% (1,773.3s) In DAA file: CH03-15.trimmed.R2.blastx.meganized.daa Output file: CH03-15.trimmed.R2.blastx.meganized.daa.daa2rma.rma6 Classifications: Taxonomy, SEED, EGGNOG, GTDB, EC, INTERPRO2GO Generating RMA6 file Parsing matches Annotating RMA6 file using FAST mode (accession database and first accession per line) Parsing file CH03-15.trimmed.R2.blastx.meganized.daa Parsing file: CH03-15.trimmed.R2.blastx.meganized.daa 10% 20% 30% 40% 50% 60% 70% 80% 90% 100% (2,628.0s) Total reads: 5,432,664 Alignments: 72,596,130 100% (0.0s) Binning reads: Initializing... Initializing binning... Using 'Naive LCA' algorithm for binning: Taxonomy Using Best-Hit algorithm for binning: SEED Using Best-Hit algorithm for binning: EGGNOG Using 'Naive LCA' algorithm for binning: GTDB Using Best-Hit algorithm for binning: EC Using Best-Hit algorithm for binning: INTERPRO2GO Binning reads... Binning reads: Analyzing alignments Total reads: 5,432,664 With hits: 5,432,664 Alignments: 72,596,130 Assig. Taxonomy: 2,127,935 Assig. SEED: 84,488 Assig. EGGNOG: 23,416 Assig. GTDB: 172,793 Assig. EC: 296,579 Assig. INTERPRO2GO: 947,892 MinSupport set to: 543 Binning reads: Applying min-support & disabled filter to Taxonomy... Min-supp. changes: 5,933 Binning reads: Applying min-support & disabled filter to GTDB... Min-supp. changes: 4,506 Binning reads: Writing classification tables Numb. Tax. classes: 326 Numb. SEED classes: 499 Numb. EGG. classes: 1,641 Numb. GTDB classes: 117 Numb. EC classes: 2,432 Numb. INT. classes: 8,729 Binning reads: Syncing Class. Taxonomy: 326 Class. SEED: 499 Class. EGGNOG: 1,641 Class. GTDB: 117 Class. EC: 2,432 Class. INTERPRO2GO: 8,729 100% (1,694.2s) Total time: 9,568.1s Peak memory: 61 of 488.3G Version MEGAN Community Edition (version 6.24.20, built 5 Feb 2023) Author(s) Daniel H. Huson Copyright (C) 2023 Daniel H. Huson. This program comes with ABSOLUTELY NO WARRANTY. Functional classifications to use: EC, EGGNOG, GTDB, INTERPRO2GO, SEED Loading ncbi.map: 2,396,736 Loading ncbi.tre: 2,396,740 Loading ec.map: 8,200 Loading ec.tre: 8,204 Loading eggnog.map: 30,875 Loading eggnog.tre: 30,986 Loading gtdb.map: 240,103 Loading gtdb.tre: 240,107 Loading interpro2go.map: 14,242 Loading interpro2go.tre: 28,907 Loading seed.map: 961 Loading seed.tre: 962 In DAA file: CH03-33.trimmed.R1.blastx.meganized.daa Output file: CH03-33.trimmed.R1.blastx.meganized.daa.daa2rma.rma6 Classifications: Taxonomy, SEED, EGGNOG, GTDB, EC, INTERPRO2GO Generating RMA6 file Parsing matches Annotating RMA6 file using FAST mode (accession database and first accession per line) Parsing file CH03-33.trimmed.R1.blastx.meganized.daa Parsing file: CH03-33.trimmed.R1.blastx.meganized.daa 10% 20% 30% 40% 50% 60% 70% 80% 90% 100% (4,093.7s) Total reads: 4,886,982 Alignments: 71,520,983 100% (0.0s) Binning reads: Initializing... Initializing binning... Using 'Naive LCA' algorithm for binning: Taxonomy Using Best-Hit algorithm for binning: SEED Using Best-Hit algorithm for binning: EGGNOG Using 'Naive LCA' algorithm for binning: GTDB Using Best-Hit algorithm for binning: EC Using Best-Hit algorithm for binning: INTERPRO2GO Binning reads... Binning reads: Analyzing alignments Total reads: 4,886,982 With hits: 4,886,982 Alignments: 71,520,983 Assig. Taxonomy: 2,293,449 Assig. SEED: 66,185 Assig. EGGNOG: 30,679 Assig. GTDB: 145,621 Assig. EC: 271,290 Assig. INTERPRO2GO: 1,041,229 MinSupport set to: 488 Binning reads: Applying min-support & disabled filter to Taxonomy... Min-supp. changes: 7,893 Binning reads: Applying min-support & disabled filter to GTDB... Min-supp. changes: 6,155 Binning reads: Writing classification tables Numb. Tax. classes: 362 Numb. SEED classes: 560 Numb. EGG. classes: 1,847 Numb. GTDB classes: 132 Numb. EC classes: 2,619 Numb. INT. classes: 8,895 Binning reads: Syncing Class. Taxonomy: 362 Class. SEED: 560 Class. EGGNOG: 1,847 Class. GTDB: 132 Class. EC: 2,619 Class. INTERPRO2GO: 8,895 100% (1,752.7s) In DAA file: CH03-33.trimmed.R2.blastx.meganized.daa Output file: CH03-33.trimmed.R2.blastx.meganized.daa.daa2rma.rma6 Classifications: Taxonomy, SEED, EGGNOG, GTDB, EC, INTERPRO2GO Generating RMA6 file Parsing matches Annotating RMA6 file using FAST mode (accession database and first accession per line) Parsing file CH03-33.trimmed.R2.blastx.meganized.daa Parsing file: CH03-33.trimmed.R2.blastx.meganized.daa 10% 20% 30% 40% 50% 60% 70% 80% 90% 100% (2,714.9s) Total reads: 4,962,456 Alignments: 71,540,834 100% (0.0s) Binning reads: Initializing... Initializing binning... Using 'Naive LCA' algorithm for binning: Taxonomy Using Best-Hit algorithm for binning: SEED Using Best-Hit algorithm for binning: EGGNOG Using 'Naive LCA' algorithm for binning: GTDB Using Best-Hit algorithm for binning: EC Using Best-Hit algorithm for binning: INTERPRO2GO Binning reads... Binning reads: Analyzing alignments Total reads: 4,962,456 With hits: 4,962,456 Alignments: 71,540,834 Assig. Taxonomy: 2,297,366 Assig. SEED: 66,430 Assig. EGGNOG: 30,964 Assig. GTDB: 147,410 Assig. EC: 271,227 Assig. INTERPRO2GO: 1,041,847 MinSupport set to: 496 Binning reads: Applying min-support & disabled filter to Taxonomy... Min-supp. changes: 7,791 Binning reads: Applying min-support & disabled filter to GTDB... Min-supp. changes: 6,111 Binning reads: Writing classification tables Numb. Tax. classes: 358 Numb. SEED classes: 556 Numb. EGG. classes: 1,864 Numb. GTDB classes: 133 Numb. EC classes: 2,603 Numb. INT. classes: 8,854 Binning reads: Syncing Class. Taxonomy: 358 Class. SEED: 556 Class. EGGNOG: 1,864 Class. GTDB: 133 Class. EC: 2,603 Class. INTERPRO2GO: 8,854 100% (1,648.2s) Total time: 10,221.0s Peak memory: 71.1 of 488.3G Version MEGAN Community Edition (version 6.24.20, built 5 Feb 2023) Author(s) Daniel H. Huson Copyright (C) 2023 Daniel H. Huson. This program comes with ABSOLUTELY NO WARRANTY. Functional classifications to use: EC, EGGNOG, GTDB, INTERPRO2GO, SEED Loading ncbi.map: 2,396,736 Loading ncbi.tre: 2,396,740 Loading ec.map: 8,200 Loading ec.tre: 8,204 Loading eggnog.map: 30,875 Loading eggnog.tre: 30,986 Loading gtdb.map: 240,103 Loading gtdb.tre: 240,107 Loading interpro2go.map: 14,242 Loading interpro2go.tre: 28,907 Loading seed.map: 961 Loading seed.tre: 962 In DAA file: CH05-01.trimmed.R1.blastx.meganized.daa Output file: CH05-01.trimmed.R1.blastx.meganized.daa.daa2rma.rma6 Classifications: Taxonomy, SEED, EGGNOG, GTDB, EC, INTERPRO2GO Generating RMA6 file Parsing matches Annotating RMA6 file using FAST mode (accession database and first accession per line) Parsing file CH05-01.trimmed.R1.blastx.meganized.daa Parsing file: CH05-01.trimmed.R1.blastx.meganized.daa 10% 20% 30% 40% 50% 60% 70% 80% 90% 100% (2,657.6s) Total reads: 4,982,296 Alignments: 74,550,832 100% (0.0s) Binning reads: Initializing... Initializing binning... Using 'Naive LCA' algorithm for binning: Taxonomy Using Best-Hit algorithm for binning: SEED Using Best-Hit algorithm for binning: EGGNOG Using 'Naive LCA' algorithm for binning: GTDB Using Best-Hit algorithm for binning: EC Using Best-Hit algorithm for binning: INTERPRO2GO Binning reads... Binning reads: Analyzing alignments Total reads: 4,982,296 With hits: 4,982,296 Alignments: 74,550,832 Assig. Taxonomy: 2,189,076 Assig. SEED: 49,083 Assig. EGGNOG: 15,464 Assig. GTDB: 91,118 Assig. EC: 216,059 Assig. INTERPRO2GO: 982,766 MinSupport set to: 498 Binning reads: Applying min-support & disabled filter to Taxonomy... Min-supp. changes: 4,725 Binning reads: Applying min-support & disabled filter to GTDB... Min-supp. changes: 3,007 Binning reads: Writing classification tables Numb. Tax. classes: 318 Numb. SEED classes: 472 Numb. EGG. classes: 1,552 Numb. GTDB classes: 94 Numb. EC classes: 2,241 Numb. INT. classes: 8,526 Binning reads: Syncing Class. Taxonomy: 318 Class. SEED: 472 Class. EGGNOG: 1,552 Class. GTDB: 94 Class. EC: 2,241 Class. INTERPRO2GO: 8,526 100% (1,779.2s) In DAA file: CH05-01.trimmed.R2.blastx.meganized.daa Output file: CH05-01.trimmed.R2.blastx.meganized.daa.daa2rma.rma6 Classifications: Taxonomy, SEED, EGGNOG, GTDB, EC, INTERPRO2GO Generating RMA6 file Parsing matches Annotating RMA6 file using FAST mode (accession database and first accession per line) Parsing file CH05-01.trimmed.R2.blastx.meganized.daa Parsing file: CH05-01.trimmed.R2.blastx.meganized.daa 10% 20% 30% 40% 50% 60% 70% 80% 90% 100% (2,272.1s) Total reads: 5,032,259 Alignments: 74,470,634 100% (0.0s) Binning reads: Initializing... Initializing binning... Using 'Naive LCA' algorithm for binning: Taxonomy Using Best-Hit algorithm for binning: SEED Using Best-Hit algorithm for binning: EGGNOG Using 'Naive LCA' algorithm for binning: GTDB Using Best-Hit algorithm for binning: EC Using Best-Hit algorithm for binning: INTERPRO2GO Binning reads... Binning reads: Analyzing alignments Total reads: 5,032,259 With hits: 5,032,259 Alignments: 74,470,634 Assig. Taxonomy: 2,186,972 Assig. SEED: 49,256 Assig. EGGNOG: 15,627 Assig. GTDB: 91,894 Assig. EC: 215,875 Assig. INTERPRO2GO: 982,334 MinSupport set to: 503 Binning reads: Applying min-support & disabled filter to Taxonomy... Min-supp. changes: 4,651 Binning reads: Applying min-support & disabled filter to GTDB... Min-supp. changes: 2,967 Binning reads: Writing classification tables Numb. Tax. classes: 321 Numb. SEED classes: 474 Numb. EGG. classes: 1,557 Numb. GTDB classes: 93 Numb. EC classes: 2,245 Numb. INT. classes: 8,509 Binning reads: Syncing Class. Taxonomy: 321 Class. SEED: 474 Class. EGGNOG: 1,557 Class. GTDB: 93 Class. EC: 2,245 Class. INTERPRO2GO: 8,509 100% (1,736.3s) Total time: 8,456.7s Peak memory: 64.9 of 488.3G Version MEGAN Community Edition (version 6.24.20, built 5 Feb 2023) Author(s) Daniel H. Huson Copyright (C) 2023 Daniel H. Huson. This program comes with ABSOLUTELY NO WARRANTY. Functional classifications to use: EC, EGGNOG, GTDB, INTERPRO2GO, SEED Loading ncbi.map: 2,396,736 Loading ncbi.tre: 2,396,740 Loading ec.map: 8,200 Loading ec.tre: 8,204 Loading eggnog.map: 30,875 Loading eggnog.tre: 30,986 Loading gtdb.map: 240,103 Loading gtdb.tre: 240,107 Loading interpro2go.map: 14,242 Loading interpro2go.tre: 28,907 Loading seed.map: 961 Loading seed.tre: 962 In DAA file: CH05-06.trimmed.R1.blastx.meganized.daa Output file: CH05-06.trimmed.R1.blastx.meganized.daa.daa2rma.rma6 Classifications: Taxonomy, SEED, EGGNOG, GTDB, EC, INTERPRO2GO Generating RMA6 file Parsing matches Annotating RMA6 file using FAST mode (accession database and first accession per line) Parsing file CH05-06.trimmed.R1.blastx.meganized.daa Parsing file: CH05-06.trimmed.R1.blastx.meganized.daa 10% 20% 30% 40% 50% 60% 70% 80% 90% 100% (3,203.1s) Total reads: 4,177,884 Alignments: 73,588,905 100% (0.0s) Binning reads: Initializing... Initializing binning... Using 'Naive LCA' algorithm for binning: Taxonomy Using Best-Hit algorithm for binning: SEED Using Best-Hit algorithm for binning: EGGNOG Using 'Naive LCA' algorithm for binning: GTDB Using Best-Hit algorithm for binning: EC Using Best-Hit algorithm for binning: INTERPRO2GO Binning reads... Binning reads: Analyzing alignments Total reads: 4,177,884 With hits: 4,177,884 Alignments: 73,588,905 Assig. Taxonomy: 2,155,587 Assig. SEED: 74,094 Assig. EGGNOG: 19,392 Assig. GTDB: 119,017 Assig. EC: 233,632 Assig. INTERPRO2GO: 1,011,753 MinSupport set to: 417 Binning reads: Applying min-support & disabled filter to Taxonomy... Min-supp. changes: 5,181 Binning reads: Applying min-support & disabled filter to GTDB... Min-supp. changes: 3,692 Binning reads: Writing classification tables Numb. Tax. classes: 313 Numb. SEED classes: 474 Numb. EGG. classes: 1,621 Numb. GTDB classes: 104 Numb. EC classes: 2,330 Numb. INT. classes: 8,426 Binning reads: Syncing Class. Taxonomy: 313 Class. SEED: 474 Class. EGGNOG: 1,621 Class. GTDB: 104 Class. EC: 2,330 Class. INTERPRO2GO: 8,426 100% (1,779.1s) In DAA file: CH05-06.trimmed.R2.blastx.meganized.daa Output file: CH05-06.trimmed.R2.blastx.meganized.daa.daa2rma.rma6 Classifications: Taxonomy, SEED, EGGNOG, GTDB, EC, INTERPRO2GO Generating RMA6 file Parsing matches Annotating RMA6 file using FAST mode (accession database and first accession per line) Parsing file CH05-06.trimmed.R2.blastx.meganized.daa Parsing file: CH05-06.trimmed.R2.blastx.meganized.daa 10% 20% 30% 40% 50% 60% 70% 80% 90% 100% (3,116.7s) Total reads: 4,203,725 Alignments: 73,423,085 100% (0.0s) Binning reads: Initializing... Initializing binning... Using 'Naive LCA' algorithm for binning: Taxonomy Using Best-Hit algorithm for binning: SEED Using Best-Hit algorithm for binning: EGGNOG Using 'Naive LCA' algorithm for binning: GTDB Using Best-Hit algorithm for binning: EC Using Best-Hit algorithm for binning: INTERPRO2GO Binning reads... Binning reads: Analyzing alignments Total reads: 4,203,725 With hits: 4,203,725 Alignments: 73,423,085 Assig. Taxonomy: 2,158,396 Assig. SEED: 74,227 Assig. EGGNOG: 19,412 Assig. GTDB: 120,229 Assig. EC: 233,402 Assig. INTERPRO2GO: 1,012,553 MinSupport set to: 420 Binning reads: Applying min-support & disabled filter to Taxonomy... Min-supp. changes: 5,111 Binning reads: Applying min-support & disabled filter to GTDB... Min-supp. changes: 3,680 Binning reads: Writing classification tables Numb. Tax. classes: 309 Numb. SEED classes: 474 Numb. EGG. classes: 1,622 Numb. GTDB classes: 107 Numb. EC classes: 2,326 Numb. INT. classes: 8,386 Binning reads: Syncing Class. Taxonomy: 309 Class. SEED: 474 Class. EGGNOG: 1,622 Class. GTDB: 107 Class. EC: 2,326 Class. INTERPRO2GO: 8,386 100% (1,692.6s) Total time: 9,803.2s Peak memory: 88 of 488.3G Version MEGAN Community Edition (version 6.24.20, built 5 Feb 2023) Author(s) Daniel H. Huson Copyright (C) 2023 Daniel H. Huson. This program comes with ABSOLUTELY NO WARRANTY. Functional classifications to use: EC, EGGNOG, GTDB, INTERPRO2GO, SEED Loading ncbi.map: 2,396,736 Loading ncbi.tre: 2,396,740 Loading ec.map: 8,200 Loading ec.tre: 8,204 Loading eggnog.map: 30,875 Loading eggnog.tre: 30,986 Loading gtdb.map: 240,103 Loading gtdb.tre: 240,107 Loading interpro2go.map: 14,242 Loading interpro2go.tre: 28,907 Loading seed.map: 961 Loading seed.tre: 962 In DAA file: CH05-07.trimmed.R1.blastx.meganized.daa Output file: CH05-07.trimmed.R1.blastx.meganized.daa.daa2rma.rma6 Classifications: Taxonomy, SEED, EGGNOG, GTDB, EC, INTERPRO2GO Generating RMA6 file Parsing matches Annotating RMA6 file using FAST mode (accession database and first accession per line) Parsing file CH05-07.trimmed.R1.blastx.meganized.daa Parsing file: CH05-07.trimmed.R1.blastx.meganized.daa 10% 20% 30% 40% 50% 60% 70% 80% 90% 100% (2,411.0s) Total reads: 4,887,125 Alignments: 67,733,261 100% (0.0s) Binning reads: Initializing... Initializing binning... Using 'Naive LCA' algorithm for binning: Taxonomy Using Best-Hit algorithm for binning: SEED Using Best-Hit algorithm for binning: EGGNOG Using 'Naive LCA' algorithm for binning: GTDB Using Best-Hit algorithm for binning: EC Using Best-Hit algorithm for binning: INTERPRO2GO Binning reads... Binning reads: Analyzing alignments Total reads: 4,887,125 With hits: 4,887,125 Alignments: 67,733,261 Assig. Taxonomy: 2,202,997 Assig. SEED: 49,923 Assig. EGGNOG: 17,764 Assig. GTDB: 78,683 Assig. EC: 233,693 Assig. INTERPRO2GO: 904,144 MinSupport set to: 488 Binning reads: Applying min-support & disabled filter to Taxonomy... Min-supp. changes: 4,479 Binning reads: Applying min-support & disabled filter to GTDB... Min-supp. changes: 2,893 Binning reads: Writing classification tables Numb. Tax. classes: 342 Numb. SEED classes: 480 Numb. EGG. classes: 1,544 Numb. GTDB classes: 95 Numb. EC classes: 2,197 Numb. INT. classes: 8,572 Binning reads: Syncing Class. Taxonomy: 342 Class. SEED: 480 Class. EGGNOG: 1,544 Class. GTDB: 95 Class. EC: 2,197 Class. INTERPRO2GO: 8,572 100% (1,705.1s) In DAA file: CH05-07.trimmed.R2.blastx.meganized.daa Output file: CH05-07.trimmed.R2.blastx.meganized.daa.daa2rma.rma6 Classifications: Taxonomy, SEED, EGGNOG, GTDB, EC, INTERPRO2GO Generating RMA6 file Parsing matches Annotating RMA6 file using FAST mode (accession database and first accession per line) Parsing file CH05-07.trimmed.R2.blastx.meganized.daa Parsing file: CH05-07.trimmed.R2.blastx.meganized.daa 10% 20% 30% 40% 50% 60% 70% 80% 90% 100% (4,496.8s) Total reads: 4,963,230 Alignments: 67,798,796 100% (0.0s) Binning reads: Initializing... Initializing binning... Using 'Naive LCA' algorithm for binning: Taxonomy Using Best-Hit algorithm for binning: SEED Using Best-Hit algorithm for binning: EGGNOG Using 'Naive LCA' algorithm for binning: GTDB Using Best-Hit algorithm for binning: EC Using Best-Hit algorithm for binning: INTERPRO2GO Binning reads... Binning reads: Analyzing alignments Total reads: 4,963,230 With hits: 4,963,230 Alignments: 67,798,796 Assig. Taxonomy: 2,205,233 Assig. SEED: 49,862 Assig. EGGNOG: 17,725 Assig. GTDB: 78,489 Assig. EC: 233,537 Assig. INTERPRO2GO: 905,708 MinSupport set to: 496 Binning reads: Applying min-support & disabled filter to Taxonomy... Min-supp. changes: 4,384 Binning reads: Applying min-support & disabled filter to GTDB... Min-supp. changes: 2,834 Binning reads: Writing classification tables Numb. Tax. classes: 337 Numb. SEED classes: 479 Numb. EGG. classes: 1,535 Numb. GTDB classes: 92 Numb. EC classes: 2,198 Numb. INT. classes: 8,553 Binning reads: Syncing Class. Taxonomy: 337 Class. SEED: 479 Class. EGGNOG: 1,535 Class. GTDB: 92 Class. EC: 2,198 Class. INTERPRO2GO: 8,553 100% (1,647.2s) Total time: 10,268.1s Peak memory: 51.7 of 488.3G Version MEGAN Community Edition (version 6.24.20, built 5 Feb 2023) Author(s) Daniel H. Huson Copyright (C) 2023 Daniel H. Huson. This program comes with ABSOLUTELY NO WARRANTY. Functional classifications to use: EC, EGGNOG, GTDB, INTERPRO2GO, SEED Loading ncbi.map: 2,396,736 Loading ncbi.tre: 2,396,740 Loading ec.map: 8,200 Loading ec.tre: 8,204 Loading eggnog.map: 30,875 Loading eggnog.tre: 30,986 Loading gtdb.map: 240,103 Loading gtdb.tre: 240,107 Loading interpro2go.map: 14,242 Loading interpro2go.tre: 28,907 Loading seed.map: 961 Loading seed.tre: 962 In DAA file: CH05-09.trimmed.R1.blastx.meganized.daa Output file: CH05-09.trimmed.R1.blastx.meganized.daa.daa2rma.rma6 Classifications: Taxonomy, SEED, EGGNOG, GTDB, EC, INTERPRO2GO Generating RMA6 file Parsing matches Annotating RMA6 file using FAST mode (accession database and first accession per line) Parsing file CH05-09.trimmed.R1.blastx.meganized.daa Parsing file: CH05-09.trimmed.R1.blastx.meganized.daa 10% 20% 30% 40% 50% 60% 70% 80% 90% 100% (3,849.0s) Total reads: 6,539,025 Alignments: 116,036,703 100% (0.0s) Binning reads: Initializing... Initializing binning... Using 'Naive LCA' algorithm for binning: Taxonomy Using Best-Hit algorithm for binning: SEED Using Best-Hit algorithm for binning: EGGNOG Using 'Naive LCA' algorithm for binning: GTDB Using Best-Hit algorithm for binning: EC Using Best-Hit algorithm for binning: INTERPRO2GO Binning reads... Binning reads: Analyzing alignments Total reads: 6,539,025 With hits: 6,539,025 Alignments: 116,036,703 Assig. Taxonomy: 3,291,577 Assig. SEED: 58,550 Assig. EGGNOG: 18,144 Assig. GTDB: 119,561 Assig. EC: 324,587 Assig. INTERPRO2GO: 1,581,531 MinSupport set to: 653 Binning reads: Applying min-support & disabled filter to Taxonomy... Min-supp. changes: 5,251 Binning reads: Applying min-support & disabled filter to GTDB... Min-supp. changes: 3,202 Binning reads: Writing classification tables Numb. Tax. classes: 310 Numb. SEED classes: 471 Numb. EGG. classes: 1,461 Numb. GTDB classes: 90 Numb. EC classes: 2,244 Numb. INT. classes: 8,679 Binning reads: Syncing Class. Taxonomy: 310 Class. SEED: 471 Class. EGGNOG: 1,461 Class. GTDB: 90 Class. EC: 2,244 Class. INTERPRO2GO: 8,679 100% (2,659.0s) In DAA file: CH05-09.trimmed.R2.blastx.meganized.daa Output file: CH05-09.trimmed.R2.blastx.meganized.daa.daa2rma.rma6 Classifications: Taxonomy, SEED, EGGNOG, GTDB, EC, INTERPRO2GO Generating RMA6 file Parsing matches Annotating RMA6 file using FAST mode (accession database and first accession per line) Parsing file CH05-09.trimmed.R2.blastx.meganized.daa Parsing file: CH05-09.trimmed.R2.blastx.meganized.daa 10% 20% 30% 40% 50% 60% 70% 80% 90% 100% (5,827.4s) Total reads: 6,624,320 Alignments: 116,279,269 100% (0.0s) Binning reads: Initializing... Initializing binning... Using 'Naive LCA' algorithm for binning: Taxonomy Using Best-Hit algorithm for binning: SEED Using Best-Hit algorithm for binning: EGGNOG Using 'Naive LCA' algorithm for binning: GTDB Using Best-Hit algorithm for binning: EC Using Best-Hit algorithm for binning: INTERPRO2GO Binning reads... Binning reads: Analyzing alignments Total reads: 6,624,320 With hits: 6,624,320 Alignments: 116,279,269 Assig. Taxonomy: 3,300,006 Assig. SEED: 58,147 Assig. EGGNOG: 17,967 Assig. GTDB: 120,614 Assig. EC: 324,529 Assig. INTERPRO2GO: 1,584,023 MinSupport set to: 662 Binning reads: Applying min-support & disabled filter to Taxonomy... Min-supp. changes: 4,989 Binning reads: Applying min-support & disabled filter to GTDB... Min-supp. changes: 3,112 Binning reads: Writing classification tables Numb. Tax. classes: 313 Numb. SEED classes: 474 Numb. EGG. classes: 1,461 Numb. GTDB classes: 90 Numb. EC classes: 2,265 Numb. INT. classes: 8,630 Binning reads: Syncing Class. Taxonomy: 313 Class. SEED: 474 Class. EGGNOG: 1,461 Class. GTDB: 90 Class. EC: 2,265 Class. INTERPRO2GO: 8,630 100% (2,592.4s) Total time: 14,939.7s Peak memory: 103.9 of 488.3G Version MEGAN Community Edition (version 6.24.20, built 5 Feb 2023) Author(s) Daniel H. Huson Copyright (C) 2023 Daniel H. Huson. This program comes with ABSOLUTELY NO WARRANTY. Functional classifications to use: EC, EGGNOG, GTDB, INTERPRO2GO, SEED Loading ncbi.map: 2,396,736 Loading ncbi.tre: 2,396,740 Loading ec.map: 8,200 Loading ec.tre: 8,204 Loading eggnog.map: 30,875 Loading eggnog.tre: 30,986 Loading gtdb.map: 240,103 Loading gtdb.tre: 240,107 Loading interpro2go.map: 14,242 Loading interpro2go.tre: 28,907 Loading seed.map: 961 Loading seed.tre: 962 In DAA file: CH05-14.trimmed.R1.blastx.meganized.daa Output file: CH05-14.trimmed.R1.blastx.meganized.daa.daa2rma.rma6 Classifications: Taxonomy, SEED, EGGNOG, GTDB, EC, INTERPRO2GO Generating RMA6 file Parsing matches Annotating RMA6 file using FAST mode (accession database and first accession per line) Parsing file CH05-14.trimmed.R1.blastx.meganized.daa Parsing file: CH05-14.trimmed.R1.blastx.meganized.daa 10% 20% 30% 40% 50% 60% 70% 80% 90% 100% (4,339.5s) Total reads: 6,231,795 Alignments: 133,642,791 100% (0.0s) Binning reads: Initializing... Initializing binning... Using 'Naive LCA' algorithm for binning: Taxonomy Using Best-Hit algorithm for binning: SEED Using Best-Hit algorithm for binning: EGGNOG Using 'Naive LCA' algorithm for binning: GTDB Using Best-Hit algorithm for binning: EC Using Best-Hit algorithm for binning: INTERPRO2GO Binning reads... Binning reads: Analyzing alignments Total reads: 6,231,795 With hits: 6,231,795 Alignments: 133,642,791 Assig. Taxonomy: 4,005,803 Assig. SEED: 62,374 Assig. EGGNOG: 11,973 Assig. GTDB: 98,997 Assig. EC: 425,422 Assig. INTERPRO2GO: 1,880,427 MinSupport set to: 623 Binning reads: Applying min-support & disabled filter to Taxonomy... Min-supp. changes: 5,117 Binning reads: Applying min-support & disabled filter to GTDB... Min-supp. changes: 3,145 Binning reads: Writing classification tables Numb. Tax. classes: 321 Numb. SEED classes: 471 Numb. EGG. classes: 1,440 Numb. GTDB classes: 91 Numb. EC classes: 2,255 Numb. INT. classes: 8,639 Binning reads: Syncing Class. Taxonomy: 321 Class. SEED: 471 Class. EGGNOG: 1,440 Class. GTDB: 91 Class. EC: 2,255 Class. INTERPRO2GO: 8,639 100% (2,951.7s) In DAA file: CH05-14.trimmed.R2.blastx.meganized.daa Output file: CH05-14.trimmed.R2.blastx.meganized.daa.daa2rma.rma6 Classifications: Taxonomy, SEED, EGGNOG, GTDB, EC, INTERPRO2GO Generating RMA6 file Parsing matches Annotating RMA6 file using FAST mode (accession database and first accession per line) Parsing file CH05-14.trimmed.R2.blastx.meganized.daa Parsing file: CH05-14.trimmed.R2.blastx.meganized.daa 10% 20% 30% 40% 50% 60% 70% 80% 90% 100% (5,506.5s) Total reads: 6,269,570 Alignments: 133,789,724 100% (0.0s) Binning reads: Initializing... Initializing binning... Using 'Naive LCA' algorithm for binning: Taxonomy Using Best-Hit algorithm for binning: SEED Using Best-Hit algorithm for binning: EGGNOG Using 'Naive LCA' algorithm for binning: GTDB Using Best-Hit algorithm for binning: EC Using Best-Hit algorithm for binning: INTERPRO2GO Binning reads... Binning reads: Analyzing alignments Total reads: 6,269,570 With hits: 6,269,570 Alignments: 133,789,724 Assig. Taxonomy: 4,013,691 Assig. SEED: 62,388 Assig. EGGNOG: 12,229 Assig. GTDB: 99,956 Assig. EC: 426,114 Assig. INTERPRO2GO: 1,882,483 MinSupport set to: 626 Binning reads: Applying min-support & disabled filter to Taxonomy... Min-supp. changes: 4,912 Binning reads: Applying min-support & disabled filter to GTDB... Min-supp. changes: 3,030 Binning reads: Writing classification tables Numb. Tax. classes: 323 Numb. SEED classes: 464 Numb. EGG. classes: 1,460 Numb. GTDB classes: 91 Numb. EC classes: 2,249 Numb. INT. classes: 8,572 Binning reads: Syncing Class. Taxonomy: 323 Class. SEED: 464 Class. EGGNOG: 1,460 Class. GTDB: 91 Class. EC: 2,249 Class. INTERPRO2GO: 8,572 100% (2,910.8s) Total time: 15,720.3s Peak memory: 95 of 488.3G Version MEGAN Community Edition (version 6.24.20, built 5 Feb 2023) Author(s) Daniel H. Huson Copyright (C) 2023 Daniel H. Huson. This program comes with ABSOLUTELY NO WARRANTY. Functional classifications to use: EC, EGGNOG, GTDB, INTERPRO2GO, SEED Loading ncbi.map: 2,396,736 Loading ncbi.tre: 2,396,740 Loading ec.map: 8,200 Loading ec.tre: 8,204 Loading eggnog.map: 30,875 Loading eggnog.tre: 30,986 Loading gtdb.map: 240,103 Loading gtdb.tre: 240,107 Loading interpro2go.map: 14,242 Loading interpro2go.tre: 28,907 Loading seed.map: 961 Loading seed.tre: 962 In DAA file: CH05-21.trimmed.R1.blastx.meganized.daa Output file: CH05-21.trimmed.R1.blastx.meganized.daa.daa2rma.rma6 Classifications: Taxonomy, SEED, EGGNOG, GTDB, EC, INTERPRO2GO Generating RMA6 file Parsing matches Annotating RMA6 file using FAST mode (accession database and first accession per line) Parsing file CH05-21.trimmed.R1.blastx.meganized.daa Parsing file: CH05-21.trimmed.R1.blastx.meganized.daa 10% 20% 30% 40% 50% 60% 70% 80% 90% 100% (4,353.4s) Total reads: 6,028,642 Alignments: 113,080,064 100% (0.0s) Binning reads: Initializing... Initializing binning... Using 'Naive LCA' algorithm for binning: Taxonomy Using Best-Hit algorithm for binning: SEED Using Best-Hit algorithm for binning: EGGNOG Using 'Naive LCA' algorithm for binning: GTDB Using Best-Hit algorithm for binning: EC Using Best-Hit algorithm for binning: INTERPRO2GO Binning reads... Binning reads: Analyzing alignments Total reads: 6,028,642 With hits: 6,028,642 Alignments: 113,080,064 Assig. Taxonomy: 3,107,401 Assig. SEED: 62,726 Assig. EGGNOG: 9,213 Assig. GTDB: 76,873 Assig. EC: 340,931 Assig. INTERPRO2GO: 1,526,151 MinSupport set to: 602 Binning reads: Applying min-support & disabled filter to Taxonomy... Min-supp. changes: 4,686 Binning reads: Applying min-support & disabled filter to GTDB... Min-supp. changes: 2,811 Binning reads: Writing classification tables Numb. Tax. classes: 278 Numb. SEED classes: 457 Numb. EGG. classes: 1,359 Numb. GTDB classes: 75 Numb. EC classes: 2,207 Numb. INT. classes: 8,434 Binning reads: Syncing Class. Taxonomy: 278 Class. SEED: 457 Class. EGGNOG: 1,359 Class. GTDB: 75 Class. EC: 2,207 Class. INTERPRO2GO: 8,434 100% (2,584.2s) In DAA file: CH05-21.trimmed.R2.blastx.meganized.daa Output file: CH05-21.trimmed.R2.blastx.meganized.daa.daa2rma.rma6 Classifications: Taxonomy, SEED, EGGNOG, GTDB, EC, INTERPRO2GO Generating RMA6 file Parsing matches Annotating RMA6 file using FAST mode (accession database and first accession per line) Parsing file CH05-21.trimmed.R2.blastx.meganized.daa Parsing file: CH05-21.trimmed.R2.blastx.meganized.daa 10% 20% 30% 40% 50% 60% 70% 80% 90% 100% (5,150.6s) Total reads: 6,066,063 Alignments: 113,112,653 100% (0.0s) Binning reads: Initializing... Initializing binning... Using 'Naive LCA' algorithm for binning: Taxonomy Using Best-Hit algorithm for binning: SEED Using Best-Hit algorithm for binning: EGGNOG Using 'Naive LCA' algorithm for binning: GTDB Using Best-Hit algorithm for binning: EC Using Best-Hit algorithm for binning: INTERPRO2GO Binning reads... Binning reads: Analyzing alignments Total reads: 6,066,063 With hits: 6,066,063 Alignments: 113,112,653 Assig. Taxonomy: 3,112,164 Assig. SEED: 62,405 Assig. EGGNOG: 9,211 Assig. GTDB: 77,294 Assig. EC: 340,716 Assig. INTERPRO2GO: 1,526,104 MinSupport set to: 606 Binning reads: Applying min-support & disabled filter to Taxonomy... Min-supp. changes: 4,579 Binning reads: Applying min-support & disabled filter to GTDB... Min-supp. changes: 2,791 Binning reads: Writing classification tables Numb. Tax. classes: 269 Numb. SEED classes: 454 Numb. EGG. classes: 1,368 Numb. GTDB classes: 74 Numb. EC classes: 2,196 Numb. INT. classes: 8,364 Binning reads: Syncing Class. Taxonomy: 269 Class. SEED: 454 Class. EGGNOG: 1,368 Class. GTDB: 74 Class. EC: 2,196 Class. INTERPRO2GO: 8,364 100% (2,528.8s) Total time: 14,628.9s Peak memory: 107.3 of 488.3G Version MEGAN Community Edition (version 6.24.20, built 5 Feb 2023) Author(s) Daniel H. Huson Copyright (C) 2023 Daniel H. Huson. This program comes with ABSOLUTELY NO WARRANTY. Functional classifications to use: EC, EGGNOG, GTDB, INTERPRO2GO, SEED Loading ncbi.map: 2,396,736 Loading ncbi.tre: 2,396,740 Loading ec.map: 8,200 Loading ec.tre: 8,204 Loading eggnog.map: 30,875 Loading eggnog.tre: 30,986 Loading gtdb.map: 240,103 Loading gtdb.tre: 240,107 Loading interpro2go.map: 14,242 Loading interpro2go.tre: 28,907 Loading seed.map: 961 Loading seed.tre: 962 In DAA file: CH05-29.trimmed.R1.blastx.meganized.daa Output file: CH05-29.trimmed.R1.blastx.meganized.daa.daa2rma.rma6 Classifications: Taxonomy, SEED, EGGNOG, GTDB, EC, INTERPRO2GO Generating RMA6 file Parsing matches Annotating RMA6 file using FAST mode (accession database and first accession per line) Parsing file CH05-29.trimmed.R1.blastx.meganized.daa Parsing file: CH05-29.trimmed.R1.blastx.meganized.daa 10% 20% 30% 40% 50% 60% 70% 80% 90% 100% (4,616.7s) Total reads: 9,995,972 Alignments: 130,650,140 100% (0.0s) Binning reads: Initializing... Initializing binning... Using 'Naive LCA' algorithm for binning: Taxonomy Using Best-Hit algorithm for binning: SEED Using Best-Hit algorithm for binning: EGGNOG Using 'Naive LCA' algorithm for binning: GTDB Using Best-Hit algorithm for binning: EC Using Best-Hit algorithm for binning: INTERPRO2GO Binning reads... Binning reads: Analyzing alignments Total reads: 9,995,972 With hits: 9,995,972 Alignments: 130,650,140 Assig. Taxonomy: 7,400,126 Assig. SEED: 68,681 Assig. EGGNOG: 27,290 Assig. GTDB: 450,974 Assig. EC: 239,781 Assig. INTERPRO2GO: 1,827,597 MinSupport set to: 999 Binning reads: Applying min-support & disabled filter to Taxonomy... Min-supp. changes: 5,420 Binning reads: Applying min-support & disabled filter to GTDB... Min-supp. changes: 3,772 Binning reads: Writing classification tables Numb. Tax. classes: 396 Numb. SEED classes: 471 Numb. EGG. classes: 1,614 Numb. GTDB classes: 102 Numb. EC classes: 2,363 Numb. INT. classes: 8,678 Binning reads: Syncing Class. Taxonomy: 396 Class. SEED: 471 Class. EGGNOG: 1,614 Class. GTDB: 102 Class. EC: 2,363 Class. INTERPRO2GO: 8,678 100% (3,186.2s) In DAA file: CH05-29.trimmed.R2.blastx.meganized.daa Output file: CH05-29.trimmed.R2.blastx.meganized.daa.daa2rma.rma6 Classifications: Taxonomy, SEED, EGGNOG, GTDB, EC, INTERPRO2GO Generating RMA6 file Parsing matches Annotating RMA6 file using FAST mode (accession database and first accession per line) Parsing file CH05-29.trimmed.R2.blastx.meganized.daa Parsing file: CH05-29.trimmed.R2.blastx.meganized.daa 10% 20% 30% 40% 50% 60% 70% 80% 90% 100% (5,652.3s) Total reads: 10,022,751 Alignments: 130,403,193 100% (0.0s) Binning reads: Initializing... Initializing binning... Using 'Naive LCA' algorithm for binning: Taxonomy Using Best-Hit algorithm for binning: SEED Using Best-Hit algorithm for binning: EGGNOG Using 'Naive LCA' algorithm for binning: GTDB Using Best-Hit algorithm for binning: EC Using Best-Hit algorithm for binning: INTERPRO2GO Binning reads... Binning reads: Analyzing alignments Total reads: 10,022,751 With hits: 10,022,751 Alignments: 130,403,193 Assig. Taxonomy: 7,443,469 Assig. SEED: 68,714 Assig. EGGNOG: 26,993 Assig. GTDB: 443,653 Assig. EC: 239,201 Assig. INTERPRO2GO: 1,818,117 MinSupport set to: 1002 Binning reads: Applying min-support & disabled filter to Taxonomy... Min-supp. changes: 5,496 Binning reads: Applying min-support & disabled filter to GTDB... Min-supp. changes: 3,673 Binning reads: Writing classification tables Numb. Tax. classes: 402 Numb. SEED classes: 476 Numb. EGG. classes: 1,608 Numb. GTDB classes: 102 Numb. EC classes: 2,374 Numb. INT. classes: 8,682 Binning reads: Syncing Class. Taxonomy: 402 Class. SEED: 476 Class. EGGNOG: 1,608 Class. GTDB: 102 Class. EC: 2,374 Class. INTERPRO2GO: 8,682 100% (3,139.8s) Total time: 16,606.4s Peak memory: 108.5 of 488.3G Version MEGAN Community Edition (version 6.24.20, built 5 Feb 2023) Author(s) Daniel H. Huson Copyright (C) 2023 Daniel H. Huson. This program comes with ABSOLUTELY NO WARRANTY. Functional classifications to use: EC, EGGNOG, GTDB, INTERPRO2GO, SEED Loading ncbi.map: 2,396,736 Loading ncbi.tre: 2,396,740 Loading ec.map: 8,200 Loading ec.tre: 8,204 Loading eggnog.map: 30,875 Loading eggnog.tre: 30,986 Loading gtdb.map: 240,103 Loading gtdb.tre: 240,107 Loading interpro2go.map: 14,242 Loading interpro2go.tre: 28,907 Loading seed.map: 961 Loading seed.tre: 962 In DAA file: CH07-04.trimmed.R1.blastx.meganized.daa Output file: CH07-04.trimmed.R1.blastx.meganized.daa.daa2rma.rma6 Classifications: Taxonomy, SEED, EGGNOG, GTDB, EC, INTERPRO2GO Generating RMA6 file Parsing matches Annotating RMA6 file using FAST mode (accession database and first accession per line) Parsing file CH07-04.trimmed.R1.blastx.meganized.daa Parsing file: CH07-04.trimmed.R1.blastx.meganized.daa 10% 20% 30% 40% 50% 60% 70% 80% 90% 100% (4,051.1s) Total reads: 3,547,598 Alignments: 71,562,624 100% (0.0s) Binning reads: Initializing... Initializing binning... Using 'Naive LCA' algorithm for binning: Taxonomy Using Best-Hit algorithm for binning: SEED Using Best-Hit algorithm for binning: EGGNOG Using 'Naive LCA' algorithm for binning: GTDB Using Best-Hit algorithm for binning: EC Using Best-Hit algorithm for binning: INTERPRO2GO Binning reads... Binning reads: Analyzing alignments Total reads: 3,547,598 With hits: 3,547,598 Alignments: 71,562,624 Assig. Taxonomy: 2,338,216 Assig. SEED: 78,230 Assig. EGGNOG: 29,799 Assig. GTDB: 167,511 Assig. EC: 250,613 Assig. INTERPRO2GO: 952,919 MinSupport set to: 354 Binning reads: Applying min-support & disabled filter to Taxonomy... Min-supp. changes: 6,334 Binning reads: Applying min-support & disabled filter to GTDB... Min-supp. changes: 4,875 Binning reads: Writing classification tables Numb. Tax. classes: 411 Numb. SEED classes: 503 Numb. EGG. classes: 1,797 Numb. GTDB classes: 132 Numb. EC classes: 2,560 Numb. INT. classes: 8,877 Binning reads: Syncing Class. Taxonomy: 411 Class. SEED: 503 Class. EGGNOG: 1,797 Class. GTDB: 132 Class. EC: 2,560 Class. INTERPRO2GO: 8,877 100% (1,658.5s) In DAA file: CH07-04.trimmed.R2.blastx.meganized.daa Output file: CH07-04.trimmed.R2.blastx.meganized.daa.daa2rma.rma6 Classifications: Taxonomy, SEED, EGGNOG, GTDB, EC, INTERPRO2GO Generating RMA6 file Parsing matches Annotating RMA6 file using FAST mode (accession database and first accession per line) Parsing file CH07-04.trimmed.R2.blastx.meganized.daa Parsing file: CH07-04.trimmed.R2.blastx.meganized.daa 10% 20% 30% 40% 50% 60% 70% 80% 90% 100% (2,547.5s) Total reads: 3,572,438 Alignments: 71,506,485 100% (0.0s) Binning reads: Initializing... Initializing binning... Using 'Naive LCA' algorithm for binning: Taxonomy Using Best-Hit algorithm for binning: SEED Using Best-Hit algorithm for binning: EGGNOG Using 'Naive LCA' algorithm for binning: GTDB Using Best-Hit algorithm for binning: EC Using Best-Hit algorithm for binning: INTERPRO2GO Binning reads... Binning reads: Analyzing alignments Total reads: 3,572,438 With hits: 3,572,438 Alignments: 71,506,485 Assig. Taxonomy: 2,338,513 Assig. SEED: 78,894 Assig. EGGNOG: 30,575 Assig. GTDB: 170,513 Assig. EC: 251,120 Assig. INTERPRO2GO: 952,091 MinSupport set to: 357 Binning reads: Applying min-support & disabled filter to Taxonomy... Min-supp. changes: 6,156 Binning reads: Applying min-support & disabled filter to GTDB... Min-supp. changes: 4,857 Binning reads: Writing classification tables Numb. Tax. classes: 407 Numb. SEED classes: 506 Numb. EGG. classes: 1,805 Numb. GTDB classes: 134 Numb. EC classes: 2,557 Numb. INT. classes: 8,887 Binning reads: Syncing Class. Taxonomy: 407 Class. SEED: 506 Class. EGGNOG: 1,805 Class. GTDB: 134 Class. EC: 2,557 Class. INTERPRO2GO: 8,887 100% (1,591.9s) Total time: 9,860.6s Peak memory: 94.7 of 488.3G Version MEGAN Community Edition (version 6.24.20, built 5 Feb 2023) Author(s) Daniel H. Huson Copyright (C) 2023 Daniel H. Huson. This program comes with ABSOLUTELY NO WARRANTY. Functional classifications to use: EC, EGGNOG, GTDB, INTERPRO2GO, SEED Loading ncbi.map: 2,396,736 Loading ncbi.tre: 2,396,740 Loading ec.map: 8,200 Loading ec.tre: 8,204 Loading eggnog.map: 30,875 Loading eggnog.tre: 30,986 Loading gtdb.map: 240,103 Loading gtdb.tre: 240,107 Loading interpro2go.map: 14,242 Loading interpro2go.tre: 28,907 Loading seed.map: 961 Loading seed.tre: 962 In DAA file: CH07-06.trimmed.R1.blastx.meganized.daa Output file: CH07-06.trimmed.R1.blastx.meganized.daa.daa2rma.rma6 Classifications: Taxonomy, SEED, EGGNOG, GTDB, EC, INTERPRO2GO Generating RMA6 file Parsing matches Annotating RMA6 file using FAST mode (accession database and first accession per line) Parsing file CH07-06.trimmed.R1.blastx.meganized.daa Parsing file: CH07-06.trimmed.R1.blastx.meganized.daa 10% 20% 30% 40% 50% 60% 70% 80% 90% 100% (3,753.6s) Total reads: 5,278,696 Alignments: 99,913,751 100% (0.0s) Binning reads: Initializing... Initializing binning... Using 'Naive LCA' algorithm for binning: Taxonomy Using Best-Hit algorithm for binning: SEED Using Best-Hit algorithm for binning: EGGNOG Using 'Naive LCA' algorithm for binning: GTDB Using Best-Hit algorithm for binning: EC Using Best-Hit algorithm for binning: INTERPRO2GO Binning reads... Binning reads: Analyzing alignments Total reads: 5,278,696 With hits: 5,278,696 Alignments: 99,913,751 Assig. Taxonomy: 3,070,836 Assig. SEED: 110,389 Assig. EGGNOG: 61,047 Assig. GTDB: 266,985 Assig. EC: 306,604 Assig. INTERPRO2GO: 1,391,130 MinSupport set to: 527 Binning reads: Applying min-support & disabled filter to Taxonomy... Min-supp. changes: 5,760 Binning reads: Applying min-support & disabled filter to GTDB... Min-supp. changes: 3,796 Binning reads: Writing classification tables Numb. Tax. classes: 342 Numb. SEED classes: 490 Numb. EGG. classes: 1,708 Numb. GTDB classes: 115 Numb. EC classes: 2,354 Numb. INT. classes: 8,727 Binning reads: Syncing Class. Taxonomy: 342 Class. SEED: 490 Class. EGGNOG: 1,708 Class. GTDB: 115 Class. EC: 2,354 Class. INTERPRO2GO: 8,727 100% (2,279.2s) In DAA file: CH07-06.trimmed.R2.blastx.meganized.daa Output file: CH07-06.trimmed.R2.blastx.meganized.daa.daa2rma.rma6 Classifications: Taxonomy, SEED, EGGNOG, GTDB, EC, INTERPRO2GO Generating RMA6 file Parsing matches Annotating RMA6 file using FAST mode (accession database and first accession per line) Parsing file CH07-06.trimmed.R2.blastx.meganized.daa Parsing file: CH07-06.trimmed.R2.blastx.meganized.daa 10% 20% 30% 40% 50% 60% 70% 80% 90% 100% (4,732.4s) Total reads: 5,326,628 Alignments: 99,762,339 100% (0.0s) Binning reads: Initializing... Initializing binning... Using 'Naive LCA' algorithm for binning: Taxonomy Using Best-Hit algorithm for binning: SEED Using Best-Hit algorithm for binning: EGGNOG Using 'Naive LCA' algorithm for binning: GTDB Using Best-Hit algorithm for binning: EC Using Best-Hit algorithm for binning: INTERPRO2GO Binning reads... Binning reads: Analyzing alignments Total reads: 5,326,628 With hits: 5,326,628 Alignments: 99,762,339 Assig. Taxonomy: 3,077,592 Assig. SEED: 111,189 Assig. EGGNOG: 61,428 Assig. GTDB: 269,887 Assig. EC: 308,002 Assig. INTERPRO2GO: 1,391,583 MinSupport set to: 532 Binning reads: Applying min-support & disabled filter to Taxonomy... Min-supp. changes: 5,614 Binning reads: Applying min-support & disabled filter to GTDB... Min-supp. changes: 3,751 Binning reads: Writing classification tables Numb. Tax. classes: 339 Numb. SEED classes: 495 Numb. EGG. classes: 1,710 Numb. GTDB classes: 115 Numb. EC classes: 2,347 Numb. INT. classes: 8,716 Binning reads: Syncing Class. Taxonomy: 339 Class. SEED: 495 Class. EGGNOG: 1,710 Class. GTDB: 115 Class. EC: 2,347 Class. INTERPRO2GO: 8,716 100% (2,230.4s) Total time: 13,007.3s Peak memory: 96.5 of 488.3G Version MEGAN Community Edition (version 6.24.20, built 5 Feb 2023) Author(s) Daniel H. Huson Copyright (C) 2023 Daniel H. Huson. This program comes with ABSOLUTELY NO WARRANTY. Functional classifications to use: EC, EGGNOG, GTDB, INTERPRO2GO, SEED Loading ncbi.map: 2,396,736 Loading ncbi.tre: 2,396,740 Loading ec.map: 8,200 Loading ec.tre: 8,204 Loading eggnog.map: 30,875 Loading eggnog.tre: 30,986 Loading gtdb.map: 240,103 Loading gtdb.tre: 240,107 Loading interpro2go.map: 14,242 Loading interpro2go.tre: 28,907 Loading seed.map: 961 Loading seed.tre: 962 In DAA file: CH07-08.trimmed.R1.blastx.meganized.daa Output file: CH07-08.trimmed.R1.blastx.meganized.daa.daa2rma.rma6 Classifications: Taxonomy, SEED, EGGNOG, GTDB, EC, INTERPRO2GO Generating RMA6 file Parsing matches Annotating RMA6 file using FAST mode (accession database and first accession per line) Parsing file CH07-08.trimmed.R1.blastx.meganized.daa Parsing file: CH07-08.trimmed.R1.blastx.meganized.daa 10% 20% 30% 40% 50% 60% 70% 80% 90% 100% (7,171.1s) Total reads: 9,400,852 Alignments: 205,228,198 100% (0.0s) Binning reads: Initializing... Initializing binning... Using 'Naive LCA' algorithm for binning: Taxonomy Using Best-Hit algorithm for binning: SEED Using Best-Hit algorithm for binning: EGGNOG Using 'Naive LCA' algorithm for binning: GTDB Using Best-Hit algorithm for binning: EC Using Best-Hit algorithm for binning: INTERPRO2GO Binning reads... Binning reads: Analyzing alignments Total reads: 9,400,852 With hits: 9,400,852 Alignments: 205,228,198 Assig. Taxonomy: 5,818,238 Assig. SEED: 78,207 Assig. EGGNOG: 16,388 Assig. GTDB: 135,895 Assig. EC: 601,783 Assig. INTERPRO2GO: 2,897,177 MinSupport set to: 940 Binning reads: Applying min-support & disabled filter to Taxonomy... Min-supp. changes: 5,741 Binning reads: Applying min-support & disabled filter to GTDB... Min-supp. changes: 3,254 Binning reads: Writing classification tables Numb. Tax. classes: 294 Numb. SEED classes: 487 Numb. EGG. classes: 1,551 Numb. GTDB classes: 96 Numb. EC classes: 2,299 Numb. INT. classes: 8,730 Binning reads: Syncing Class. Taxonomy: 294 Class. SEED: 487 Class. EGGNOG: 1,551 Class. GTDB: 96 Class. EC: 2,299 Class. INTERPRO2GO: 8,730 100% (4,498.2s) In DAA file: CH07-08.trimmed.R2.blastx.meganized.daa Output file: CH07-08.trimmed.R2.blastx.meganized.daa.daa2rma.rma6 Classifications: Taxonomy, SEED, EGGNOG, GTDB, EC, INTERPRO2GO Generating RMA6 file Parsing matches Annotating RMA6 file using FAST mode (accession database and first accession per line) Parsing file CH07-08.trimmed.R2.blastx.meganized.daa Parsing file: CH07-08.trimmed.R2.blastx.meganized.daa 10% 20% 30% 40% 50% 60% 70% 80% 90% 100% (15,116.7s) Total reads: 9,461,735 Alignments: 205,083,201 100% (0.0s) Binning reads: Initializing... Initializing binning... Using 'Naive LCA' algorithm for binning: Taxonomy Using Best-Hit algorithm for binning: SEED Using Best-Hit algorithm for binning: EGGNOG Using 'Naive LCA' algorithm for binning: GTDB Using Best-Hit algorithm for binning: EC Using Best-Hit algorithm for binning: INTERPRO2GO Binning reads... Binning reads: Analyzing alignments Total reads: 9,461,735 With hits: 9,461,735 Alignments: 205,083,201 Assig. Taxonomy: 5,827,159 Assig. SEED: 78,644 Assig. EGGNOG: 16,590 Assig. GTDB: 138,460 Assig. EC: 601,626 Assig. INTERPRO2GO: 2,898,127 MinSupport set to: 946 Binning reads: Applying min-support & disabled filter to Taxonomy... Min-supp. changes: 5,611 Binning reads: Applying min-support & disabled filter to GTDB... Min-supp. changes: 3,261 Binning reads: Writing classification tables Numb. Tax. classes: 283 Numb. SEED classes: 487 Numb. EGG. classes: 1,559 Numb. GTDB classes: 94 Numb. EC classes: 2,296 Numb. INT. classes: 8,710 Binning reads: Syncing Class. Taxonomy: 283 Class. SEED: 487 Class. EGGNOG: 1,559 Class. GTDB: 94 Class. EC: 2,296 Class. INTERPRO2GO: 8,710 100% (4,429.7s) Total time: 31,227.4s Peak memory: 96.7 of 488.3G Version MEGAN Community Edition (version 6.24.20, built 5 Feb 2023) Author(s) Daniel H. Huson Copyright (C) 2023 Daniel H. Huson. This program comes with ABSOLUTELY NO WARRANTY. Functional classifications to use: EC, EGGNOG, GTDB, INTERPRO2GO, SEED Loading ncbi.map: 2,396,736 Loading ncbi.tre: 2,396,740 Loading ec.map: 8,200 Loading ec.tre: 8,204 Loading eggnog.map: 30,875 Loading eggnog.tre: 30,986 Loading gtdb.map: 240,103 Loading gtdb.tre: 240,107 Loading interpro2go.map: 14,242 Loading interpro2go.tre: 28,907 Loading seed.map: 961 Loading seed.tre: 962 In DAA file: CH07-11.trimmed.R1.blastx.meganized.daa Output file: CH07-11.trimmed.R1.blastx.meganized.daa.daa2rma.rma6 Classifications: Taxonomy, SEED, EGGNOG, GTDB, EC, INTERPRO2GO Generating RMA6 file Parsing matches Annotating RMA6 file using FAST mode (accession database and first accession per line) Parsing file CH07-11.trimmed.R1.blastx.meganized.daa Parsing file: CH07-11.trimmed.R1.blastx.meganized.daa 10% 20% 30% 40% 50% 60% 70% 80% 90% 100% (5,159.5s) Total reads: 7,075,583 Alignments: 106,368,293 100% (0.0s) Binning reads: Initializing... Initializing binning... Using 'Naive LCA' algorithm for binning: Taxonomy Using Best-Hit algorithm for binning: SEED Using Best-Hit algorithm for binning: EGGNOG Using 'Naive LCA' algorithm for binning: GTDB Using Best-Hit algorithm for binning: EC Using Best-Hit algorithm for binning: INTERPRO2GO Binning reads... Binning reads: Analyzing alignments Total reads: 7,075,583 With hits: 7,075,583 Alignments: 106,368,293 Assig. Taxonomy: 3,198,674 Assig. SEED: 76,172 Assig. EGGNOG: 33,852 Assig. GTDB: 164,487 Assig. EC: 276,677 Assig. INTERPRO2GO: 1,498,152 MinSupport set to: 707 Binning reads: Applying min-support & disabled filter to Taxonomy... Min-supp. changes: 6,212 Binning reads: Applying min-support & disabled filter to GTDB... Min-supp. changes: 4,310 Binning reads: Writing classification tables Numb. Tax. classes: 345 Numb. SEED classes: 500 Numb. EGG. classes: 1,670 Numb. GTDB classes: 116 Numb. EC classes: 2,396 Numb. INT. classes: 8,850 Binning reads: Syncing Class. Taxonomy: 345 Class. SEED: 500 Class. EGGNOG: 1,670 Class. GTDB: 116 Class. EC: 2,396 Class. INTERPRO2GO: 8,850 100% (2,554.2s) In DAA file: CH07-11.trimmed.R2.blastx.meganized.daa Output file: CH07-11.trimmed.R2.blastx.meganized.daa.daa2rma.rma6 Classifications: Taxonomy, SEED, EGGNOG, GTDB, EC, INTERPRO2GO Generating RMA6 file Parsing matches Annotating RMA6 file using FAST mode (accession database and first accession per line) Parsing file CH07-11.trimmed.R2.blastx.meganized.daa Parsing file: CH07-11.trimmed.R2.blastx.meganized.daa 10% 20% 30% 40% 50% 60% 70% 80% 90% 100% (6,198.3s) Total reads: 7,159,883 Alignments: 106,503,414 100% (0.0s) Binning reads: Initializing... Initializing binning... Using 'Naive LCA' algorithm for binning: Taxonomy Using Best-Hit algorithm for binning: SEED Using Best-Hit algorithm for binning: EGGNOG Using 'Naive LCA' algorithm for binning: GTDB Using Best-Hit algorithm for binning: EC Using Best-Hit algorithm for binning: INTERPRO2GO Binning reads... Binning reads: Analyzing alignments Total reads: 7,159,883 With hits: 7,159,883 Alignments: 106,503,414 Assig. Taxonomy: 3,207,250 Assig. SEED: 76,713 Assig. EGGNOG: 34,335 Assig. GTDB: 165,869 Assig. EC: 277,679 Assig. INTERPRO2GO: 1,501,692 MinSupport set to: 715 Binning reads: Applying min-support & disabled filter to Taxonomy... Min-supp. changes: 6,018 Binning reads: Applying min-support & disabled filter to GTDB... Min-supp. changes: 4,245 Binning reads: Writing classification tables Numb. Tax. classes: 349 Numb. SEED classes: 509 Numb. EGG. classes: 1,699 Numb. GTDB classes: 116 Numb. EC classes: 2,402 Numb. INT. classes: 8,806 Binning reads: Syncing Class. Taxonomy: 349 Class. SEED: 509 Class. EGGNOG: 1,699 Class. GTDB: 116 Class. EC: 2,402 Class. INTERPRO2GO: 8,806 100% (2,471.6s) Total time: 16,394.9s Peak memory: 77.5 of 488.3G Version MEGAN Community Edition (version 6.24.20, built 5 Feb 2023) Author(s) Daniel H. Huson Copyright (C) 2023 Daniel H. Huson. This program comes with ABSOLUTELY NO WARRANTY. Functional classifications to use: EC, EGGNOG, GTDB, INTERPRO2GO, SEED Loading ncbi.map: 2,396,736 Loading ncbi.tre: 2,396,740 Loading ec.map: 8,200 Loading ec.tre: 8,204 Loading eggnog.map: 30,875 Loading eggnog.tre: 30,986 Loading gtdb.map: 240,103 Loading gtdb.tre: 240,107 Loading interpro2go.map: 14,242 Loading interpro2go.tre: 28,907 Loading seed.map: 961 Loading seed.tre: 962 In DAA file: CH07-24.trimmed.R1.blastx.meganized.daa Output file: CH07-24.trimmed.R1.blastx.meganized.daa.daa2rma.rma6 Classifications: Taxonomy, SEED, EGGNOG, GTDB, EC, INTERPRO2GO Generating RMA6 file Parsing matches Annotating RMA6 file using FAST mode (accession database and first accession per line) Parsing file CH07-24.trimmed.R1.blastx.meganized.daa Parsing file: CH07-24.trimmed.R1.blastx.meganized.daa 10% 20% 30% 40% 50% 60% 70% 80% 90% 100% (8,194.7s) Total reads: 7,083,899 Alignments: 183,225,860 100% (0.0s) Binning reads: Initializing... Initializing binning... Using 'Naive LCA' algorithm for binning: Taxonomy Using Best-Hit algorithm for binning: SEED Using Best-Hit algorithm for binning: EGGNOG Using 'Naive LCA' algorithm for binning: GTDB Using Best-Hit algorithm for binning: EC Using Best-Hit algorithm for binning: INTERPRO2GO Binning reads... Binning reads: Analyzing alignments Total reads: 7,083,899 With hits: 7,083,899 Alignments: 183,225,860 Assig. Taxonomy: 4,729,257 Assig. SEED: 77,289 Assig. EGGNOG: 20,165 Assig. GTDB: 138,895 Assig. EC: 433,914 Assig. INTERPRO2GO: 2,481,813 MinSupport set to: 708 Binning reads: Applying min-support & disabled filter to Taxonomy... Min-supp. changes: 6,823 Binning reads: Applying min-support & disabled filter to GTDB... Min-supp. changes: 4,515 Binning reads: Writing classification tables Numb. Tax. classes: 321 Numb. SEED classes: 485 Numb. EGG. classes: 1,580 Numb. GTDB classes: 93 Numb. EC classes: 2,364 Numb. INT. classes: 8,768 Binning reads: Syncing Class. Taxonomy: 321 Class. SEED: 485 Class. EGGNOG: 1,580 Class. GTDB: 93 Class. EC: 2,364 Class. INTERPRO2GO: 8,768 100% (4,052.1s) In DAA file: CH07-24.trimmed.R2.blastx.meganized.daa Output file: CH07-24.trimmed.R2.blastx.meganized.daa.daa2rma.rma6 Classifications: Taxonomy, SEED, EGGNOG, GTDB, EC, INTERPRO2GO Generating RMA6 file Parsing matches Annotating RMA6 file using FAST mode (accession database and first accession per line) Parsing file CH07-24.trimmed.R2.blastx.meganized.daa Parsing file: CH07-24.trimmed.R2.blastx.meganized.daa 10% 20% 30% 40% 50% 60% 70% 80% 90% 100% (13,609.9s) Total reads: 7,114,414 Alignments: 183,162,187 100% (0.0s) Binning reads: Initializing... Initializing binning... Using 'Naive LCA' algorithm for binning: Taxonomy Using Best-Hit algorithm for binning: SEED Using Best-Hit algorithm for binning: EGGNOG Using 'Naive LCA' algorithm for binning: GTDB Using Best-Hit algorithm for binning: EC Using Best-Hit algorithm for binning: INTERPRO2GO Binning reads... Binning reads: Analyzing alignments Total reads: 7,114,414 With hits: 7,114,414 Alignments: 183,162,187 Assig. Taxonomy: 4,737,361 Assig. SEED: 76,602 Assig. EGGNOG: 20,018 Assig. GTDB: 141,361 Assig. EC: 433,884 Assig. INTERPRO2GO: 2,483,290 MinSupport set to: 711 Binning reads: Applying min-support & disabled filter to Taxonomy... Min-supp. changes: 6,576 Binning reads: Applying min-support & disabled filter to GTDB... Min-supp. changes: 4,429 Binning reads: Writing classification tables Numb. Tax. classes: 310 Numb. SEED classes: 478 Numb. EGG. classes: 1,610 Numb. GTDB classes: 95 Numb. EC classes: 2,380 Numb. INT. classes: 8,742 Binning reads: Syncing Class. Taxonomy: 310 Class. SEED: 478 Class. EGGNOG: 1,610 Class. GTDB: 95 Class. EC: 2,380 Class. INTERPRO2GO: 8,742 100% (3,889.4s) Total time: 29,757.6s Peak memory: 98.1 of 488.3G Version MEGAN Community Edition (version 6.24.20, built 5 Feb 2023) Author(s) Daniel H. Huson Copyright (C) 2023 Daniel H. Huson. This program comes with ABSOLUTELY NO WARRANTY. Functional classifications to use: EC, EGGNOG, GTDB, INTERPRO2GO, SEED Loading ncbi.map: 2,396,736 Loading ncbi.tre: 2,396,740 Loading ec.map: 8,200 Loading ec.tre: 8,204 Loading eggnog.map: 30,875 Loading eggnog.tre: 30,986 Loading gtdb.map: 240,103 Loading gtdb.tre: 240,107 Loading interpro2go.map: 14,242 Loading interpro2go.tre: 28,907 Loading seed.map: 961 Loading seed.tre: 962 In DAA file: CH09-02.trimmed.R1.blastx.meganized.daa Output file: CH09-02.trimmed.R1.blastx.meganized.daa.daa2rma.rma6 Classifications: Taxonomy, SEED, EGGNOG, GTDB, EC, INTERPRO2GO Generating RMA6 file Parsing matches Annotating RMA6 file using FAST mode (accession database and first accession per line) Parsing file CH09-02.trimmed.R1.blastx.meganized.daa Parsing file: CH09-02.trimmed.R1.blastx.meganized.daa 10% 20% 30% 40% 50% 60% 70% 80% 90% 100% (4,049.3s) Total reads: 4,240,072 Alignments: 62,342,700 100% (0.0s) Binning reads: Initializing... Initializing binning... Using 'Naive LCA' algorithm for binning: Taxonomy Using Best-Hit algorithm for binning: SEED Using Best-Hit algorithm for binning: EGGNOG Using 'Naive LCA' algorithm for binning: GTDB Using Best-Hit algorithm for binning: EC Using Best-Hit algorithm for binning: INTERPRO2GO Binning reads... Binning reads: Analyzing alignments Total reads: 4,240,072 With hits: 4,240,072 Alignments: 62,342,700 Assig. Taxonomy: 1,805,722 Assig. SEED: 51,184 Assig. EGGNOG: 15,791 Assig. GTDB: 95,653 Assig. EC: 184,022 Assig. INTERPRO2GO: 784,457 MinSupport set to: 424 Binning reads: Applying min-support & disabled filter to Taxonomy... Min-supp. changes: 5,273 Binning reads: Applying min-support & disabled filter to GTDB... Min-supp. changes: 3,831 Binning reads: Writing classification tables Numb. Tax. classes: 351 Numb. SEED classes: 498 Numb. EGG. classes: 1,571 Numb. GTDB classes: 119 Numb. EC classes: 2,409 Numb. INT. classes: 8,725 Binning reads: Syncing Class. Taxonomy: 351 Class. SEED: 498 Class. EGGNOG: 1,571 Class. GTDB: 119 Class. EC: 2,409 Class. INTERPRO2GO: 8,725 100% (1,530.9s) In DAA file: CH09-02.trimmed.R2.blastx.meganized.daa Output file: CH09-02.trimmed.R2.blastx.meganized.daa.daa2rma.rma6 Classifications: Taxonomy, SEED, EGGNOG, GTDB, EC, INTERPRO2GO Generating RMA6 file Parsing matches Annotating RMA6 file using FAST mode (accession database and first accession per line) Parsing file CH09-02.trimmed.R2.blastx.meganized.daa Parsing file: CH09-02.trimmed.R2.blastx.meganized.daa 10% 20% 30% 40% 50% 60% 70% 80% 90% 100% (2,076.6s) Total reads: 4,270,268 Alignments: 62,323,391 100% (0.0s) Binning reads: Initializing... Initializing binning... Using 'Naive LCA' algorithm for binning: Taxonomy Using Best-Hit algorithm for binning: SEED Using Best-Hit algorithm for binning: EGGNOG Using 'Naive LCA' algorithm for binning: GTDB Using Best-Hit algorithm for binning: EC Using Best-Hit algorithm for binning: INTERPRO2GO Binning reads... Binning reads: Analyzing alignments Total reads: 4,270,268 With hits: 4,270,268 Alignments: 62,323,391 Assig. Taxonomy: 1,807,225 Assig. SEED: 51,590 Assig. EGGNOG: 16,119 Assig. GTDB: 96,306 Assig. EC: 184,208 Assig. INTERPRO2GO: 784,207 MinSupport set to: 427 Binning reads: Applying min-support & disabled filter to Taxonomy... Min-supp. changes: 5,146 Binning reads: Applying min-support & disabled filter to GTDB... Min-supp. changes: 3,826 Binning reads: Writing classification tables Numb. Tax. classes: 364 Numb. SEED classes: 495 Numb. EGG. classes: 1,585 Numb. GTDB classes: 118 Numb. EC classes: 2,405 Numb. INT. classes: 8,673 Binning reads: Syncing Class. Taxonomy: 364 Class. SEED: 495 Class. EGGNOG: 1,585 Class. GTDB: 118 Class. EC: 2,405 Class. INTERPRO2GO: 8,673 100% (1,440.4s) Total time: 9,105.4s Peak memory: 48.6 of 488.3G Version MEGAN Community Edition (version 6.24.20, built 5 Feb 2023) Author(s) Daniel H. Huson Copyright (C) 2023 Daniel H. Huson. This program comes with ABSOLUTELY NO WARRANTY. Functional classifications to use: EC, EGGNOG, GTDB, INTERPRO2GO, SEED Loading ncbi.map: 2,396,736 Loading ncbi.tre: 2,396,740 Loading ec.map: 8,200 Loading ec.tre: 8,204 Loading eggnog.map: 30,875 Loading eggnog.tre: 30,986 Loading gtdb.map: 240,103 Loading gtdb.tre: 240,107 Loading interpro2go.map: 14,242 Loading interpro2go.tre: 28,907 Loading seed.map: 961 Loading seed.tre: 962 In DAA file: CH09-13.trimmed.R1.blastx.meganized.daa Output file: CH09-13.trimmed.R1.blastx.meganized.daa.daa2rma.rma6 Classifications: Taxonomy, SEED, EGGNOG, GTDB, EC, INTERPRO2GO Generating RMA6 file Parsing matches Annotating RMA6 file using FAST mode (accession database and first accession per line) Parsing file CH09-13.trimmed.R1.blastx.meganized.daa Parsing file: CH09-13.trimmed.R1.blastx.meganized.daa 10% 20% 30% 40% 50% 60% 70% 80% 90% 100% (4,731.1s) Total reads: 6,048,342 Alignments: 77,203,379 100% (0.0s) Binning reads: Initializing... Initializing binning... Using 'Naive LCA' algorithm for binning: Taxonomy Using Best-Hit algorithm for binning: SEED Using Best-Hit algorithm for binning: EGGNOG Using 'Naive LCA' algorithm for binning: GTDB Using Best-Hit algorithm for binning: EC Using Best-Hit algorithm for binning: INTERPRO2GO Binning reads... Binning reads: Analyzing alignments Total reads: 6,048,342 With hits: 6,048,342 Alignments: 77,203,379 Assig. Taxonomy: 2,766,700 Assig. SEED: 759,102 Assig. EGGNOG: 315,269 Assig. GTDB: 1,601,016 Assig. EC: 696,779 Assig. INTERPRO2GO: 889,121 MinSupport set to: 604 Binning reads: Applying min-support & disabled filter to Taxonomy... Min-supp. changes: 13,392 Binning reads: Applying min-support & disabled filter to GTDB... Min-supp. changes: 13,429 Binning reads: Writing classification tables Numb. Tax. classes: 519 Numb. SEED classes: 575 Numb. EGG. classes: 2,124 Numb. GTDB classes: 296 Numb. EC classes: 2,931 Numb. INT. classes: 9,679 Binning reads: Syncing Class. Taxonomy: 519 Class. SEED: 575 Class. EGGNOG: 2,124 Class. GTDB: 296 Class. EC: 2,931 Class. INTERPRO2GO: 9,679 100% (2,014.9s) In DAA file: CH09-13.trimmed.R2.blastx.meganized.daa Output file: CH09-13.trimmed.R2.blastx.meganized.daa.daa2rma.rma6 Classifications: Taxonomy, SEED, EGGNOG, GTDB, EC, INTERPRO2GO Generating RMA6 file Parsing matches Annotating RMA6 file using FAST mode (accession database and first accession per line) Parsing file CH09-13.trimmed.R2.blastx.meganized.daa Parsing file: CH09-13.trimmed.R2.blastx.meganized.daa 10% 20% 30% 40% 50% 60% 70% 80% 90% 100% (7,992.7s) Total reads: 6,141,769 Alignments: 77,950,246 100% (0.0s) Binning reads: Initializing... Initializing binning... Using 'Naive LCA' algorithm for binning: Taxonomy Using Best-Hit algorithm for binning: SEED Using Best-Hit algorithm for binning: EGGNOG Using 'Naive LCA' algorithm for binning: GTDB Using Best-Hit algorithm for binning: EC Using Best-Hit algorithm for binning: INTERPRO2GO Binning reads... Binning reads: Analyzing alignments Total reads: 6,141,769 With hits: 6,141,769 Alignments: 77,950,246 Assig. Taxonomy: 2,810,657 Assig. SEED: 772,477 Assig. EGGNOG: 320,302 Assig. GTDB: 1,636,464 Assig. EC: 708,782 Assig. INTERPRO2GO: 899,301 MinSupport set to: 614 Binning reads: Applying min-support & disabled filter to Taxonomy... Min-supp. changes: 13,309 Binning reads: Applying min-support & disabled filter to GTDB... Min-supp. changes: 13,455 Binning reads: Writing classification tables Numb. Tax. classes: 527 Numb. SEED classes: 574 Numb. EGG. classes: 2,124 Numb. GTDB classes: 293 Numb. EC classes: 2,932 Numb. INT. classes: 9,643 Binning reads: Syncing Class. Taxonomy: 527 Class. SEED: 574 Class. EGGNOG: 2,124 Class. GTDB: 293 Class. EC: 2,932 Class. INTERPRO2GO: 9,643 100% (1,909.6s) Total time: 16,656.6s Peak memory: 62.3 of 488.3G Version MEGAN Community Edition (version 6.24.20, built 5 Feb 2023) Author(s) Daniel H. Huson Copyright (C) 2023 Daniel H. Huson. This program comes with ABSOLUTELY NO WARRANTY. Functional classifications to use: EC, EGGNOG, GTDB, INTERPRO2GO, SEED Loading ncbi.map: 2,396,736 Loading ncbi.tre: 2,396,740 Loading ec.map: 8,200 Loading ec.tre: 8,204 Loading eggnog.map: 30,875 Loading eggnog.tre: 30,986 Loading gtdb.map: 240,103 Loading gtdb.tre: 240,107 Loading interpro2go.map: 14,242 Loading interpro2go.tre: 28,907 Loading seed.map: 961 Loading seed.tre: 962 In DAA file: CH09-28.trimmed.R1.blastx.meganized.daa Output file: CH09-28.trimmed.R1.blastx.meganized.daa.daa2rma.rma6 Classifications: Taxonomy, SEED, EGGNOG, GTDB, EC, INTERPRO2GO Generating RMA6 file Parsing matches Annotating RMA6 file using FAST mode (accession database and first accession per line) Parsing file CH09-28.trimmed.R1.blastx.meganized.daa Parsing file: CH09-28.trimmed.R1.blastx.meganized.daa 10% 20% 30% 40% 50% 60% 70% 80% 90% 100% (1,763.5s) Total reads: 4,269,168 Alignments: 54,614,525 100% (0.0s) Binning reads: Initializing... Initializing binning... Using 'Naive LCA' algorithm for binning: Taxonomy Using Best-Hit algorithm for binning: SEED Using Best-Hit algorithm for binning: EGGNOG Using 'Naive LCA' algorithm for binning: GTDB Using Best-Hit algorithm for binning: EC Using Best-Hit algorithm for binning: INTERPRO2GO Binning reads... Binning reads: Analyzing alignments Total reads: 4,269,168 With hits: 4,269,168 Alignments: 54,614,525 Assig. Taxonomy: 1,838,527 Assig. SEED: 107,430 Assig. EGGNOG: 52,416 Assig. GTDB: 240,994 Assig. EC: 216,500 Assig. INTERPRO2GO: 794,436 MinSupport set to: 426 Binning reads: Applying min-support & disabled filter to Taxonomy... Min-supp. changes: 6,945 Binning reads: Applying min-support & disabled filter to GTDB... Min-supp. changes: 5,923 Binning reads: Writing classification tables Numb. Tax. classes: 379 Numb. SEED classes: 526 Numb. EGG. classes: 1,861 Numb. GTDB classes: 155 Numb. EC classes: 2,567 Numb. INT. classes: 8,874 Binning reads: Syncing Class. Taxonomy: 379 Class. SEED: 526 Class. EGGNOG: 1,861 Class. GTDB: 155 Class. EC: 2,567 Class. INTERPRO2GO: 8,874 100% (1,431.5s) In DAA file: CH09-28.trimmed.R2.blastx.meganized.daa Output file: CH09-28.trimmed.R2.blastx.meganized.daa.daa2rma.rma6 Classifications: Taxonomy, SEED, EGGNOG, GTDB, EC, INTERPRO2GO Generating RMA6 file Parsing matches Annotating RMA6 file using FAST mode (accession database and first accession per line) Parsing file CH09-28.trimmed.R2.blastx.meganized.daa Parsing file: CH09-28.trimmed.R2.blastx.meganized.daa 10% 20% 30% 40% 50% 60% 70% 80% 90% 100% (5,717.4s) Total reads: 4,375,089 Alignments: 54,654,483 100% (0.0s) Binning reads: Initializing... Initializing binning... Using 'Naive LCA' algorithm for binning: Taxonomy Using Best-Hit algorithm for binning: SEED Using Best-Hit algorithm for binning: EGGNOG Using 'Naive LCA' algorithm for binning: GTDB Using Best-Hit algorithm for binning: EC Using Best-Hit algorithm for binning: INTERPRO2GO Binning reads... Binning reads: Analyzing alignments Total reads: 4,375,089 With hits: 4,375,089 Alignments: 54,654,483 Assig. Taxonomy: 1,843,193 Assig. SEED: 109,279 Assig. EGGNOG: 53,004 Assig. GTDB: 245,516 Assig. EC: 218,297 Assig. INTERPRO2GO: 796,793 MinSupport set to: 437 Binning reads: Applying min-support & disabled filter to Taxonomy... Min-supp. changes: 6,863 Binning reads: Applying min-support & disabled filter to GTDB... Min-supp. changes: 5,784 Binning reads: Writing classification tables Numb. Tax. classes: 381 Numb. SEED classes: 525 Numb. EGG. classes: 1,862 Numb. GTDB classes: 156 Numb. EC classes: 2,552 Numb. INT. classes: 8,832 Binning reads: Syncing Class. Taxonomy: 381 Class. SEED: 525 Class. EGGNOG: 1,862 Class. GTDB: 156 Class. EC: 2,552 Class. INTERPRO2GO: 8,832 100% (1,348.5s) Total time: 10,268.8s Peak memory: 53.3 of 488.3G Version MEGAN Community Edition (version 6.24.20, built 5 Feb 2023) Author(s) Daniel H. Huson Copyright (C) 2023 Daniel H. Huson. This program comes with ABSOLUTELY NO WARRANTY. Functional classifications to use: EC, EGGNOG, GTDB, INTERPRO2GO, SEED Loading ncbi.map: 2,396,736 Loading ncbi.tre: 2,396,740 Loading ec.map: 8,200 Loading ec.tre: 8,204 Loading eggnog.map: 30,875 Loading eggnog.tre: 30,986 Loading gtdb.map: 240,103 Loading gtdb.tre: 240,107 Loading interpro2go.map: 14,242 Loading interpro2go.tre: 28,907 Loading seed.map: 961 Loading seed.tre: 962 In DAA file: CH10-08.trimmed.R1.blastx.meganized.daa Output file: CH10-08.trimmed.R1.blastx.meganized.daa.daa2rma.rma6 Classifications: Taxonomy, SEED, EGGNOG, GTDB, EC, INTERPRO2GO Generating RMA6 file Parsing matches Annotating RMA6 file using FAST mode (accession database and first accession per line) Parsing file CH10-08.trimmed.R1.blastx.meganized.daa Parsing file: CH10-08.trimmed.R1.blastx.meganized.daa 10% 20% 30% 40% 50% 60% 70% 80% 90% 100% (3,966.1s) Total reads: 5,649,168 Alignments: 105,729,809 100% (0.0s) Binning reads: Initializing... Initializing binning... Using 'Naive LCA' algorithm for binning: Taxonomy Using Best-Hit algorithm for binning: SEED Using Best-Hit algorithm for binning: EGGNOG Using 'Naive LCA' algorithm for binning: GTDB Using Best-Hit algorithm for binning: EC Using Best-Hit algorithm for binning: INTERPRO2GO Binning reads... Binning reads: Analyzing alignments Total reads: 5,649,168 With hits: 5,649,168 Alignments: 105,729,809 Assig. Taxonomy: 3,324,108 Assig. SEED: 120,661 Assig. EGGNOG: 27,349 Assig. GTDB: 188,322 Assig. EC: 407,913 Assig. INTERPRO2GO: 1,518,951 MinSupport set to: 564 Binning reads: Applying min-support & disabled filter to Taxonomy... Min-supp. changes: 6,536 Binning reads: Applying min-support & disabled filter to GTDB... Min-supp. changes: 5,012 Binning reads: Writing classification tables Numb. Tax. classes: 335 Numb. SEED classes: 484 Numb. EGG. classes: 1,698 Numb. GTDB classes: 113 Numb. EC classes: 2,418 Numb. INT. classes: 8,777 Binning reads: Syncing Class. Taxonomy: 335 Class. SEED: 484 Class. EGGNOG: 1,698 Class. GTDB: 113 Class. EC: 2,418 Class. INTERPRO2GO: 8,777 100% (2,593.2s) In DAA file: CH10-08.trimmed.R2.blastx.meganized.daa Output file: CH10-08.trimmed.R2.blastx.meganized.daa.daa2rma.rma6 Classifications: Taxonomy, SEED, EGGNOG, GTDB, EC, INTERPRO2GO Generating RMA6 file Parsing matches Annotating RMA6 file using FAST mode (accession database and first accession per line) Parsing file CH10-08.trimmed.R2.blastx.meganized.daa Parsing file: CH10-08.trimmed.R2.blastx.meganized.daa 10% 20% 30% 40% 50% 60% 70% 80% 90% 100% (5,272.4s) Total reads: 5,681,258 Alignments: 105,657,803 100% (0.0s) Binning reads: Initializing... Initializing binning... Using 'Naive LCA' algorithm for binning: Taxonomy Using Best-Hit algorithm for binning: SEED Using Best-Hit algorithm for binning: EGGNOG Using 'Naive LCA' algorithm for binning: GTDB Using Best-Hit algorithm for binning: EC Using Best-Hit algorithm for binning: INTERPRO2GO Binning reads... Binning reads: Analyzing alignments Total reads: 5,681,258 With hits: 5,681,258 Alignments: 105,657,803 Assig. Taxonomy: 3,328,125 Assig. SEED: 120,734 Assig. EGGNOG: 27,439 Assig. GTDB: 190,878 Assig. EC: 407,916 Assig. INTERPRO2GO: 1,520,508 MinSupport set to: 568 Binning reads: Applying min-support & disabled filter to Taxonomy... Min-supp. changes: 6,434 Binning reads: Applying min-support & disabled filter to GTDB... Min-supp. changes: 5,008 Binning reads: Writing classification tables Numb. Tax. classes: 333 Numb. SEED classes: 487 Numb. EGG. classes: 1,682 Numb. GTDB classes: 113 Numb. EC classes: 2,414 Numb. INT. classes: 8,695 Binning reads: Syncing Class. Taxonomy: 333 Class. SEED: 487 Class. EGGNOG: 1,682 Class. GTDB: 113 Class. EC: 2,414 Class. INTERPRO2GO: 8,695 100% (2,507.3s) Total time: 14,350.8s Peak memory: 96.4 of 488.3G Version MEGAN Community Edition (version 6.24.20, built 5 Feb 2023) Author(s) Daniel H. Huson Copyright (C) 2023 Daniel H. Huson. This program comes with ABSOLUTELY NO WARRANTY. Functional classifications to use: EC, EGGNOG, GTDB, INTERPRO2GO, SEED Loading ncbi.map: 2,396,736 Loading ncbi.tre: 2,396,740 Loading ec.map: 8,200 Loading ec.tre: 8,204 Loading eggnog.map: 30,875 Loading eggnog.tre: 30,986 Loading gtdb.map: 240,103 Loading gtdb.tre: 240,107 Loading interpro2go.map: 14,242 Loading interpro2go.tre: 28,907 Loading seed.map: 961 Loading seed.tre: 962 In DAA file: CH10-11.trimmed.R1.blastx.meganized.daa Output file: CH10-11.trimmed.R1.blastx.meganized.daa.daa2rma.rma6 Classifications: Taxonomy, SEED, EGGNOG, GTDB, EC, INTERPRO2GO Generating RMA6 file Parsing matches Annotating RMA6 file using FAST mode (accession database and first accession per line) Parsing file CH10-11.trimmed.R1.blastx.meganized.daa Parsing file: CH10-11.trimmed.R1.blastx.meganized.daa 10% 20% 30% 40% 50% 60% 70% 80% 90% 100% (3,424.3s) Total reads: 5,343,597 Alignments: 74,978,658 100% (0.0s) Binning reads: Initializing... Initializing binning... Using 'Naive LCA' algorithm for binning: Taxonomy Using Best-Hit algorithm for binning: SEED Using Best-Hit algorithm for binning: EGGNOG Using 'Naive LCA' algorithm for binning: GTDB Using Best-Hit algorithm for binning: EC Using Best-Hit algorithm for binning: INTERPRO2GO Binning reads... Binning reads: Analyzing alignments Total reads: 5,343,597 With hits: 5,343,597 Alignments: 74,978,658 Assig. Taxonomy: 2,288,460 Assig. SEED: 75,177 Assig. EGGNOG: 20,850 Assig. GTDB: 128,768 Assig. EC: 287,694 Assig. INTERPRO2GO: 999,782 MinSupport set to: 534 Binning reads: Applying min-support & disabled filter to Taxonomy... Min-supp. changes: 5,281 Binning reads: Applying min-support & disabled filter to GTDB... Min-supp. changes: 3,899 Binning reads: Writing classification tables Numb. Tax. classes: 328 Numb. SEED classes: 474 Numb. EGG. classes: 1,602 Numb. GTDB classes: 105 Numb. EC classes: 2,362 Numb. INT. classes: 8,684 Binning reads: Syncing Class. Taxonomy: 328 Class. SEED: 474 Class. EGGNOG: 1,602 Class. GTDB: 105 Class. EC: 2,362 Class. INTERPRO2GO: 8,684 100% (1,935.2s) In DAA file: CH10-11.trimmed.R2.blastx.meganized.daa Output file: CH10-11.trimmed.R2.blastx.meganized.daa.daa2rma.rma6 Classifications: Taxonomy, SEED, EGGNOG, GTDB, EC, INTERPRO2GO Generating RMA6 file Parsing matches Annotating RMA6 file using FAST mode (accession database and first accession per line) Parsing file CH10-11.trimmed.R2.blastx.meganized.daa Parsing file: CH10-11.trimmed.R2.blastx.meganized.daa 10% 20% 30% 40% 50% 60% 70% 80% 90% 100% (3,971.4s) Total reads: 5,430,503 Alignments: 74,980,364 100% (0.0s) Binning reads: Initializing... Initializing binning... Using 'Naive LCA' algorithm for binning: Taxonomy Using Best-Hit algorithm for binning: SEED Using Best-Hit algorithm for binning: EGGNOG Using 'Naive LCA' algorithm for binning: GTDB Using Best-Hit algorithm for binning: EC Using Best-Hit algorithm for binning: INTERPRO2GO Binning reads... Binning reads: Analyzing alignments Total reads: 5,430,503 With hits: 5,430,503 Alignments: 74,980,364 Assig. Taxonomy: 2,294,908 Assig. SEED: 75,883 Assig. EGGNOG: 21,087 Assig. GTDB: 130,337 Assig. EC: 288,368 Assig. INTERPRO2GO: 1,001,990 MinSupport set to: 543 Binning reads: Applying min-support & disabled filter to Taxonomy... Min-supp. changes: 5,206 Binning reads: Applying min-support & disabled filter to GTDB... Min-supp. changes: 3,925 Binning reads: Writing classification tables Numb. Tax. classes: 324 Numb. SEED classes: 477 Numb. EGG. classes: 1,615 Numb. GTDB classes: 104 Numb. EC classes: 2,389 Numb. INT. classes: 8,704 Binning reads: Syncing Class. Taxonomy: 324 Class. SEED: 477 Class. EGGNOG: 1,615 Class. GTDB: 104 Class. EC: 2,389 Class. INTERPRO2GO: 8,704 100% (1,823.6s) Total time: 11,162.6s Peak memory: 84.3 of 488.3G ------------------------------------------ Total runtime was: 08days 198hrs 39mins 58secs