SUMMARISING RUN PARAMETERS ========================== Input filename: ctenidia_1.fastq.gz Trimming mode: paired-end Trim Galore version: 0.6.7 Cutadapt version: 3.4 Python version: could not detect Number of cores used for trimming: 4 Quality encoding type selected: ASCII+33 Using Illumina adapter for trimming (count: 2004). Second best hit was smallRNA (count: 15) Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; auto-detected) 2-colour high quality G-trimming enabled, with quality cutoff: --nextseq-trim=20 Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp Running FastQC on the data once trimming has completed Output file will be GZIP compressed This is cutadapt 3.4 with Python 3.9.6 Command line parameters: -j 4 -e 0.1 --nextseq-trim=20 -O 1 -a AGATCGGAAGAGC ctenidia_1.fastq.gz Processing reads on 4 cores in single-end mode ... Finished in 612.73 s (10 µs/read; 5.90 M reads/minute). === Summary === Total reads processed: 60,302,305 Reads with adapters: 17,514,938 (29.0%) Reads written (passing filters): 60,302,305 (100.0%) Total basepairs processed: 8,736,775,878 bp Quality-trimmed: 298,852,024 bp (3.4%) Total written (filtered): 8,151,698,965 bp (93.3%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 17514938 times No. of allowed errors: 1-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 40.0% C: 27.6% G: 11.8% T: 20.0% none/other: 0.6% Overview of removed sequences length count expect max.err error counts 1 13606444 15075576.2 0 13606444 3 709762 942223.5 0 709762 4 220866 235555.9 0 220866 5 45849 58889.0 0 45849 8 3785 920.1 0 3785 9 3852 230.0 0 3852 10 511 57.5 1 0 511 11 5023 14.4 1 2829 2194 12 2133 3.6 1 0 2133 13 3757 0.9 1 1487 2270 14 7035 0.9 1 1589 5446 15 4695 0.9 1 1544 3151 16 9180 0.9 1 3155 6025 17 8346 0.9 1 3342 5004 18 5533 0.9 1 2487 3046 19 3882 0.9 1 1704 2178 20 8490 0.9 1 3682 4808 21 5668 0.9 1 3302 2366 22 7070 0.9 1 3942 3128 23 2210 0.9 1 1428 782 24 6048 0.9 1 3351 2697 25 5100 0.9 1 2565 2535 26 6728 0.9 1 4114 2614 27 9777 0.9 1 5327 4450 28 6764 0.9 1 3487 3277 29 8909 0.9 1 6192 2717 30 2926 0.9 1 1844 1082 31 4977 0.9 1 2905 2072 32 4001 0.9 1 2743 1258 33 7433 0.9 1 5214 2219 34 4930 0.9 1 3015 1915 35 6431 0.9 1 4059 2372 36 7518 0.9 1 5016 2502 37 4215 0.9 1 3196 1019 38 7056 0.9 1 4749 2307 39 3070 0.9 1 1929 1141 40 5994 0.9 1 4036 1958 41 3335 0.9 1 2355 980 42 3889 0.9 1 2561 1328 43 2792 0.9 1 1960 832 44 4661 0.9 1 2718 1943 45 2360 0.9 1 1718 642 46 5568 0.9 1 3745 1823 47 1459 0.9 1 959 500 48 7793 0.9 1 5804 1989 49 1199 0.9 1 890 309 50 3480 0.9 1 2317 1163 51 4235 0.9 1 1659 2576 52 3133 0.9 1 2648 485 53 3443 0.9 1 2005 1438 54 2511 0.9 1 798 1713 55 2804 0.9 1 1580 1224 56 3243 0.9 1 1243 2000 57 2691 0.9 1 1258 1433 58 2317 0.9 1 1188 1129 59 1076 0.9 1 769 307 60 3258 0.9 1 1320 1938 61 1965 0.9 1 689 1276 62 3793 0.9 1 1146 2647 63 817 0.9 1 533 284 64 2434 0.9 1 1032 1402 65 3267 0.9 1 675 2592 66 3351 0.9 1 822 2529 67 3261 0.9 1 853 2408 68 1740 0.9 1 670 1070 69 1939 0.9 1 408 1531 70 2698 0.9 1 405 2293 71 4255 0.9 1 471 3784 72 8867 0.9 1 368 8499 73 47911 0.9 1 406 47505 74 178983 0.9 1 515 178468 75 246596 0.9 1 542 246054 76 196122 0.9 1 574 195548 77 206599 0.9 1 418 206181 78 180452 0.9 1 250 180202 79 143369 0.9 1 169 143200 80 114717 0.9 1 116 114601 81 89044 0.9 1 81 88963 82 67965 0.9 1 73 67892 83 69374 0.9 1 56 69318 84 59232 0.9 1 40 59192 85 47327 0.9 1 31 47296 86 40188 0.9 1 36 40152 87 34218 0.9 1 25 34193 88 26229 0.9 1 31 26198 89 22215 0.9 1 31 22184 90 18725 0.9 1 32 18693 91 15577 0.9 1 22 15555 92 12494 0.9 1 19 12475 93 10867 0.9 1 16 10851 94 9008 0.9 1 12 8996 95 7768 0.9 1 17 7751 96 7147 0.9 1 15 7132 97 6252 0.9 1 16 6236 98 5378 0.9 1 10 5368 99 4489 0.9 1 12 4477 100 4225 0.9 1 15 4210 101 4333 0.9 1 11 4322 102 4657 0.9 1 10 4647 103 5008 0.9 1 13 4995 104 5178 0.9 1 9 5169 105 5696 0.9 1 6 5690 106 5786 0.9 1 4 5782 107 6126 0.9 1 8 6118 108 6391 0.9 1 16 6375 109 6549 0.9 1 12 6537 110 6708 0.9 1 6 6702 111 6518 0.9 1 7 6511 112 6319 0.9 1 10 6309 113 5774 0.9 1 5 5769 114 5564 0.9 1 15 5549 115 5072 0.9 1 15 5057 116 4637 0.9 1 8 4629 117 4217 0.9 1 8 4209 118 3933 0.9 1 11 3922 119 3648 0.9 1 5 3643 120 3348 0.9 1 6 3342 121 3006 0.9 1 9 2997 122 3084 0.9 1 10 3074 123 2617 0.9 1 8 2609 124 2649 0.9 1 16 2633 125 2303 0.9 1 6 2297 126 2160 0.9 1 5 2155 127 2147 0.9 1 8 2139 128 2026 0.9 1 6 2020 129 1990 0.9 1 9 1981 130 1912 0.9 1 12 1900 131 1821 0.9 1 7 1814 132 1731 0.9 1 4 1727 133 1692 0.9 1 5 1687 134 1778 0.9 1 9 1769 135 1854 0.9 1 12 1842 136 1825 0.9 1 5 1820 137 1929 0.9 1 7 1922 138 2051 0.9 1 10 2041 139 2271 0.9 1 10 2261 140 2560 0.9 1 8 2552 141 2982 0.9 1 7 2975 142 3398 0.9 1 1 3397 143 4330 0.9 1 1 4329 144 5611 0.9 1 7 5604 145 7375 0.9 1 4 7371 146 10736 0.9 1 2 10734 147 19634 0.9 1 2 19632 148 43513 0.9 1 3 43510 149 72631 0.9 1 1 72630 150 316137 0.9 1 3 316134 151 137650 0.9 1 0 137650 RUN STATISTICS FOR INPUT FILE: ctenidia_1.fastq.gz ============================================= 60302305 sequences processed in total