WARNING An augustus species is mentioned in the config file, dataset default species (fly) will be ignored INFO ****************** Start a BUSCO 3.0.2 analysis, current time: 03/31/2020 09:40:25 ****************** INFO Configuration loaded from /gscratch/scrubbed/samwhite/outputs/20200207_hemat_busco_megan/config.ini INFO Init tools... INFO Check dependencies... INFO Check input file... INFO To reproduce this run: python /gscratch/srlab/programs/busco-v3/scripts/run_BUSCO.py -i /gscratch/srlab/sam/data/Hematodinium/transcriptomes/20200122.hemat.megan.Trinity.fasta -o 20200331.hemat.megan -l /gscratch/srlab/sam/data/databases/BUSCO/metazoa_odb9/ -m transcriptome -c 28 --long -z INFO Mode is: transcriptome INFO The lineage dataset is: metazoa_odb9 (eukaryota) INFO Temp directory is ./tmp/ INFO ****** Step 1/2, current time: 03/31/2020 09:40:25 ****** INFO Create blast database... INFO [makeblastdb] Building a new DB, current time: 03/31/2020 09:40:26 INFO [makeblastdb] New DB name: /gscratch/scrubbed/samwhite/outputs/20200207_hemat_busco_megan/tmp/20200331.hemat.megan_2126296182 INFO [makeblastdb] New DB title: /gscratch/srlab/sam/data/Hematodinium/transcriptomes/20200122.hemat.megan.Trinity.fasta INFO [makeblastdb] Sequence type: Nucleotide INFO [makeblastdb] Keep MBits: T INFO [makeblastdb] Maximum file size: 1000000000B INFO [makeblastdb] Adding sequences from FASTA; added 5183 sequences in 0.106651 seconds. INFO [makeblastdb] 1 of 1 task(s) completed at 03/31/2020 09:40:26 INFO Running tblastn, writing output to /gscratch/scrubbed/samwhite/outputs/20200207_hemat_busco_megan/run_20200331.hemat.megan/blast_output/tblastn_20200331.hemat.megan.tsv... INFO [tblastn] 1 of 1 task(s) completed at 03/31/2020 09:40:48 INFO ****** Step 2/2, current time: 03/31/2020 09:40:48 ****** INFO Maximum number of candidate transcript per BUSCO limited to: 3 INFO Getting coordinates for candidate transcripts... INFO Extracting candidate transcripts... INFO Translating candidate transcripts... INFO Running HMMER to confirm transcript orthology: INFO [hmmsearch] 74 of 737 task(s) completed at 03/31/2020 09:40:52 INFO [hmmsearch] 737 of 737 task(s) completed at 03/31/2020 09:40:54 INFO Results: INFO C:25.1%[S:19.2%,D:5.9%],F:9.5%,M:65.4%,n:978 INFO 246 Complete BUSCOs (C) INFO 188 Complete and single-copy BUSCOs (S) INFO 58 Complete and duplicated BUSCOs (D) INFO 93 Fragmented BUSCOs (F) INFO 639 Missing BUSCOs (M) INFO 978 Total BUSCO groups searched INFO BUSCO analysis done with WARNING(s). Total running time: 31.040421724319458 seconds INFO Results written in /gscratch/scrubbed/samwhite/outputs/20200207_hemat_busco_megan/run_20200331.hemat.megan/