Bismark report for: /gscratch/scrubbed/samwhite/data/P_generosa/BSeq/rrbs/pgen_bsseq_all_R1.fastq.gz and /gscratch/scrubbed/samwhite/data/P_generosa/BSeq/rrbs/pgen_bsseq_all_R2.fastq.gz (version: v0.19.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/scrubbed/samwhite/data/P_generosa/Pgenerosa_v073/ with the specified options: -q --score-min L,0,-0.6 -p 28 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--directional' specified (default mode): alignments to complementary strands (CTOT, CTOB) were ignored (i.e. not performed) Final Alignment report ====================== Sequence pairs analysed in total: 100000 Number of paired-end alignments with a unique best hit: 48184 Mapping efficiency: 48.2% Sequence pairs with no alignments under any condition: 40187 Sequence pairs did not map uniquely: 11629 Sequence pairs which were discarded because genomic sequence could not be extracted: 36 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 24377 ((converted) top strand) GA/CT/CT: 0 (complementary to (converted) top strand) GA/CT/GA: 0 (complementary to (converted) bottom strand) CT/GA/GA: 23771 ((converted) bottom strand) Number of alignments to (merely theoretical) complementary strands being rejected in total: 0 Final Cytosine Methylation Report ================================= Total number of C's analysed: 1536187 Total methylated C's in CpG context: 49738 Total methylated C's in CHG context: 6801 Total methylated C's in CHH context: 11943 Total methylated C's in Unknown context: 218 Total unmethylated C's in CpG context: 167533 Total unmethylated C's in CHG context: 314808 Total unmethylated C's in CHH context: 985364 Total unmethylated C's in Unknown context: 3050 C methylated in CpG context: 22.9% C methylated in CHG context: 2.1% C methylated in CHH context: 1.2% C methylated in unknown context (CN or CHN): 6.7% Bismark completed in 0d 0h 1m 34s