Bismark report for: /gscratch/scrubbed/samwhite/data/O_lurida/BSseq/whole_genome_BSseq_reads/20180830_trimgalore/8_ACTTGA_L001_R1_001_trimmed.fq.gz (version: v0.19.0) Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sam/data/O_lurida/oly_genome_assemblies/Olurida_v081/oly_v081_bismark_genome/ with the specified options: -q --score-min L,0,-0.6 -p 28 --reorder --ignore-quals Final Alignment report ====================== Sequences analysed in total: 14269815 Number of alignments with a unique best hit from the different alignments: 7189168 Mapping efficiency: 50.4% Sequences with no alignments under any condition: 2182397 Sequences did not map uniquely: 4898250 Sequences which were discarded because genomic sequence could not be extracted: 5024 Number of sequences with unique best (first) alignment came from the bowtie output: CT/CT: 3432533 ((converted) top strand) CT/GA: 3430237 ((converted) bottom strand) GA/CT: 160954 (complementary to (converted) top strand) GA/GA: 160420 (complementary to (converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 55645916 Total methylated C's in CpG context: 1989732 Total methylated C's in CHG context: 163325 Total methylated C's in CHH context: 1904554 Total methylated C's in Unknown context: 35445 Total unmethylated C's in CpG context: 6151369 Total unmethylated C's in CHG context: 10732784 Total unmethylated C's in CHH context: 34704152 Total unmethylated C's in Unknown context: 190719 C methylated in CpG context: 24.4% C methylated in CHG context: 1.5% C methylated in CHH context: 5.2% C methylated in Unknown context (CN or CHN): 15.7% Bismark completed in 0d 14h 56m 55s